Communication
J. Nutr. Sci. Vitaminol., 24, 589-591, 1978
EFFECTS
OF ON
PANTOTHENIC
ACID
HYALURONIC
ACID
MALE
Yusuke
VITAMIN
CONTENT
MOUSE
KANKE, Kazuharu
AND
B6
IN
SKIN
SUZUKI, and
Shiro
GOTOl
Department of Nutrition, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156, Japan (Received July 24, 1978)
The effect of vitamin C on the biosynthesis of GAG has been studied by many investigators (1-5), but no information is available as to the effect of water-soluble vitamins other than vitamin C on GAG metabolism. The present paper will describe evidence suggesting that PA and B6 cause a significant increase in HA content in skin connective tissue. D-Pantothenic acid calcium salt (PA) was obtained from Tokyo Kasei Kogyo Co., Ltd. (Tokyo, Japan). Pyridoxine hydrochloride (B6) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Male mice of the dd/Y strain (18-21g) were fed with standard laboratory pellets and tap water ad libitum throughout the experiment. The animals were divided into three groups and received vitamin subcutaneously in 0.1ml of 0.9% NaCI once daily for 7 days. Group 1 was the control for vitamin treatment and was given the vehicle (0.9% NaCl) only. Groups 2 and 3 received 1mg of PA and 2mg of B6, respectively. The
back
skin
subcutaneous solution were
fat and
treated
dialyzed
crude
with
fractions various
have
for
by
and
described
initial 3,
body the
GAG,
body
and
and
obtained
The
detailed
in
our
weight
the
addition
of
all
used
were
GP,
pantothenic acid; B6, vitamin B6. 1 菅 家 祐 輔,鈴 木 和 春,五 島 孜 郎 589
the
of
the
samples
methods
about
19.5g.
into
The
used
of
and
dialyzed
separation
chloride. further
papain
protein,
for
cetylpyridium
papers
gains
glycosaminoglycans;
remainder
and
with
undigested Aliquots
were
clipped
mixed
digestion,
the water.
here
animals
closely
were
procedures
previous of
been
papain
precipitate distilled
GP)
weight
had samples
Following to
fraction
hair dried
24hr.
water
"total"
the
fat-free
acid,
fractions
GAG
been
2 and
Abbreviations:
60•Ž
components.
mean 1,
The
tap
for GP
("total,"
The
at
after
out.
trichloroacetic
saved and
chemical
analysis
removed
running
were GAG
groups
incubated
against
samples
was
dissected
for and
three
assay
of
chemical
(6-8). was
2.1g,
3.2g
glycopeptides;
and
HA,
1.4g,
In
animals
of
respectively,
hyaluronic
acid;
PA,
590
Y. KANKE,
Table
1.
Effects
K. SUZUKI,
of vitamins
on the contents
components
a Values acid. c
are Vitamin
expressed B6.
substances (GAG and * Significantly different
as ƒÊg/100mg d Papain GP). e from
in male
dry digest
and
tissue
before
Glycosaminoglycans. control (group 1)
mouse
and separation
S. GOTO
of connective
tissue
skin.a
represent into
the the
mean•}SE two
.
b Pantothenic
hexosamine-containing
f Glycopeptides. , p
J. Nutr. Sci. Vitaminol., 24, 589-591, 1978
EFFECTS
OF ON
PANTOTHENIC
ACID
HYALURONIC
ACID
MALE
Yusuke
VITAMIN
CONTENT
MOUSE
KANKE, Kazuharu
AND
B6
IN
SKIN
SUZUKI, and
Shiro
GOTOl
Department of Nutrition, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156, Japan (Received July 24, 1978)
The effect of vitamin C on the biosynthesis of GAG has been studied by many investigators (1-5), but no information is available as to the effect of water-soluble vitamins other than vitamin C on GAG metabolism. The present paper will describe evidence suggesting that PA and B6 cause a significant increase in HA content in skin connective tissue. D-Pantothenic acid calcium salt (PA) was obtained from Tokyo Kasei Kogyo Co., Ltd. (Tokyo, Japan). Pyridoxine hydrochloride (B6) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Male mice of the dd/Y strain (18-21g) were fed with standard laboratory pellets and tap water ad libitum throughout the experiment. The animals were divided into three groups and received vitamin subcutaneously in 0.1ml of 0.9% NaCI once daily for 7 days. Group 1 was the control for vitamin treatment and was given the vehicle (0.9% NaCl) only. Groups 2 and 3 received 1mg of PA and 2mg of B6, respectively. The
back
skin
subcutaneous solution were
fat and
treated
dialyzed
crude
with
fractions various
have
for
by
and
described
initial 3,
body the
GAG,
body
and
and
obtained
The
detailed
in
our
weight
the
addition
of
all
used
were
GP,
pantothenic acid; B6, vitamin B6. 1 菅 家 祐 輔,鈴 木 和 春,五 島 孜 郎 589
the
of
the
samples
methods
about
19.5g.
into
The
used
of
and
dialyzed
separation
chloride. further
papain
protein,
for
cetylpyridium
papers
gains
glycosaminoglycans;
remainder
and
with
undigested Aliquots
were
clipped
mixed
digestion,
the water.
here
animals
closely
were
procedures
previous of
been
papain
precipitate distilled
GP)
weight
had samples
Following to
fraction
hair dried
24hr.
water
"total"
the
fat-free
acid,
fractions
GAG
been
2 and
Abbreviations:
60•Ž
components.
mean 1,
The
tap
for GP
("total,"
The
at
after
out.
trichloroacetic
saved and
chemical
analysis
removed
running
were GAG
groups
incubated
against
samples
was
dissected
for and
three
assay
of
chemical
(6-8). was
2.1g,
3.2g
glycopeptides;
and
HA,
1.4g,
In
animals
of
respectively,
hyaluronic
acid;
PA,
590
Y. KANKE,
Table
1.
Effects
K. SUZUKI,
of vitamins
on the contents
components
a Values acid. c
are Vitamin
expressed B6.
substances (GAG and * Significantly different
as ƒÊg/100mg d Papain GP). e from
in male
dry digest
and
tissue
before
Glycosaminoglycans. control (group 1)
mouse
and separation
S. GOTO
of connective
tissue
skin.a
represent into
the the
mean•}SE two
.
b Pantothenic
hexosamine-containing
f Glycopeptides. , p
