J. Nutr.

Effects

of

Sesamin

and

Elongation

of in

Yoko

Curcumin

Fatty

Cultured

FUJIYAMA-FUJIWARA,

Institute

Rat

Rumi

of Environmental

Science

2-1-1,

Ohtsuka,

Effects

chain rat

elongation primary

for

(n-6),

amount

of

sesamin

An

addition

concentrations

tion

of

C20

and

to

results

C22

n-6

fatty

more

Key

acid,

and Words

The

but

oil

of

‡™5-desaturase - desaturase

fungi

(5),

dihomo-ƒÁ-linolenic

fatty

and

the but

chain

the

elonga

corresponding of

n-3

and

curcumin.

n-6 These

inhibited ‡™5-desaturation

in

essential

acids

fatty

rat

hepatocytes;

elongation

2)

was

also

curcumin

inhibited

by

acid-rich

oil (3,

was

found that n-6

in

rat as

to

PUFA. liver

substrates

is

influenced

(1,

4).

2).

The

increase

sesamin,

chain

addition

the ƒÁ-linolenic

Shimizu microsome

353

by

of

et al.

sesame reported

preparation In

whole

many

Mortierella

a lignan

(6).

elongation,

rat

metabolism

acids

conditions

for

sesamin

acid

biological

suggesting

activity by

sesamin

chain

the

C20

curcumin,‡™5-desaturation,

and

this

acid

3)

increased

into

from

in

elongation

respectively,

carbons)

curcumin

fatty

polyunsaturated

accumulate ƒÁ-linolenic

produced

n-3

sesamin;

chain

(n-3)

the

sesamin

curcumin.

metabolism

of

with and

and with

than

inhibited

18

with

increased

18:4

elongation

inhibited

not

than

constituents

medium

the

incubated

with

in

culture

incubation

accumulate,

(n-3),

curcumin with

sesamin

sesamin,

hepatocytes,

dietary

also

that: 1)

effective

sesamin

or

acids

Similarly,

was

suggested

fatty

of

not

effective

or 20:4

to

when

(n-6)

(n-6) or

24h

did

interfered

(n-6)

studied

added

after

more

and

were

was

whereas

was

18:3

sesamin

(the

acids.

sesamin

hepatocytes

curcumin

of

University,

Japan

(PUFA)

(n-6)20:4

20:3

of

acids

C18

and

Ochanomizu

on ‡™5-desaturation

acid

(n-3),

20:3

IGARASHI

1992)

curcumin

Curcumin

of

addition C18

families

was

of

Both

simultaneous

of

ratio added.

PUFAs.

cellular

20:4

Chain

Osamu

112,

by ‡™5-desaturation

sesamin

respect.

17,

When

from

metabolite

the

Life,

fatty

rat

1992

Metabolism

and

Tokyo

and

(n-3),

(n-3) the

consequently,

of

20:4

20:5

20:3

sesamin

hepatocytes.

containing

produced

Human

April

and

Acid

UMEDA,

polyunsaturated

cultured

medium

this

of

of

38, 353-363,

Hepatocytes

Bunkyo-ku,

(Received

Summary

Vitaminol.,

on ‡™5-Desaturation

Polyunsaturated

Primary

Sci.

animal

factors

alpina of

such

sesamin acid seed the

in

oil,

in

inhibits

inhibition

the

to

culture

content

the the

of ‡™5

using ƒÁ-linolenic studies,

as

is known

and addition

of

354

Y. FUJIYAMA-FUJIWARA

sesamin the

into

fatty

diets

acid

- desaturase

decreased

activity

Also curry

in

it has

been

reported

inhibited

in

effects

of

and

sesamin

primary

to

of

system

in

on

the

rat

PUFAs

fatty

acids

99%

purchased

of

these

by

Co.,

(Grand

Island,

Chemical

the

Ltd.

Co.

rat

Co.,

Ltd.,

of

(200g,

for

collagenase

in

a

95%

incubation.

We

Experimental

air-5% used

respectively

0.5mM.

Sesamin

CO2 neither

the

and and

added

curcumin

concentration

on

into Ltd.,

free)

nor

All

of

to

the

were of

are chain

and

to

All

sesamin

from

DSF2980) Japan).

Nissui

(FBS)

trypsin

and

donated

(Osaka,

was

(n

Pharma

from

from

Gibco

Wako

(Osaka,

Pure Japan).

inhibitor

(soybean,

(USA). were chow et

60mmƒÓ

al.

Japan)

at

acids

for

and

3•~106

the

a

Clea detailed

isolated

cells

by

licenced

per

dish

with

maintained

experiments

after

at a

17-h

medium.

dissolved

and and

the

Co.,

at the final

ethanol

from

Nippon

(Corning

in were

medium

12.5ƒÊg/ml

(14)

(10mg/liter),

used

in

prepared

(CE-2,

Hepatocytes

dish

gentamicin

culture

20:5

Lot.

Co.

(12).

fatty

also

from

dexamethasone

the

was

serum

previously

dissolved up

18:4

Japan). of

Co.

Nakamura

and

and

reagent,

Hepatocytes

atmosphere insulin

(13)

commercial

a

and

al.

purchased

Ltd.

Tokyo,

FBS

been

and

mixture

Schering-Plough

the of

(n-6) (Tokyo,

histolyticum)

hepatocytes.

Co., 10%

has

hepatocytes

Ltd.

equal

bovine

acid

described

procedure.

solution,

at

Glass,

in

PUFAs

after

desaturation

Industry

fetal

Co.,

seeded

containing

et

was

from

method

PUFAs

cells

20:4

(guaranteed

and

fed

been

were

is Iwaki DME

the has

perfusion

manufacturer of

by

culture

an

Chemical

fatty

rat

of the

on

PUFAs rat

on

Co.,

(DME)

Sigma

strain)

series data

series

(n-3),

(Clostridium

from

cultured

Tokyo)

procedure

Pure

(essentially

Wistar

20:4

Fukuda

gentamicin

purchased

Preparation male

and

albumin

were

of

Japan)

metabolism

METHODS

Curcumin

Collagenase

in the

investigated

n-3

cultured

Sesamin,

medium

(Tokyo,

NY).

serum I-S)

37•Ž

Wako

the

cultured

n-3

constituents

(n-6),

Ltd.

used

increased

we

and

in of

Petrochemical

method

Eagle

Industry

Bovine

of ‡™5

reaction.

purity.

Co.,

modified

ceutical

20:3

n-6

primary

food

affect paper

PUFAs

AND

Idemitsu

more

from

Dulbecco's

medium

or

Petrochemical

was

3ml

by

prepared

Idemitsu

type

(n-6),

donated

had

episesamin,

changed

turmeric

and

fundamental

metabolism (12),

of

the

including ‡™5-desaturase

18:3

kindly

and

decrease

of (9)

this of

obtain

the

hepatocytes effects

In

metabolism

to

Since

alpina

may

animals.

activity

11).

the

Chemicals. were

(7)

the

oil.

MATERIALS

- 3)

rats

by

pigment

M.

constituents

of

desaturation

elucidate

elongation

in

probably

a yellow

food

hepatocytes

(10,

easily

suitable

cholesterol

of

mould

some

curcumin

rat

previously

proceeded

vitro

and

The

reported

of

curcumin,

this

that

in

cultured

metabolism.

in

suggest

vivo

that

acid

results

PUFAs

level

phospholipids

the ‡™5-desaturase

of ƒÁ-linolenic These

plasma

liver

(8).

powder,

content

the

composition

et al.

in

20%

BSA

concentration added

7.5ƒÊg/ml,

J. Nutr.

to

the

of culture

respectively.

Sci.

Vitaminol.

INHIBITION

Hepatocytes

OF

were

incubated

of ‡™5-desaturation, - 3)

for

that

and of

chain

of

cellular

lipids

were

Analysis

of

of

margaric

capillary the

PUFAs'

an

analysis. The

Results

are

significance

of

protein

were

the

which

conditions

measured

by

presented

as

differences

of

fatty

FID

using 200nmol

contents

Rascot

of

in

Lowry of

values

was

were

Silar

described

mean•}SD

mean

by An and

with

acid

and

method

the

(n

250.

methylated (12),

were

the

20:5 washed

determination

paper

with

or

were

(15).

extracts

Then

measurement

Sonifier

et al.

previous

355

(n-6)

Branson

Folch

the

the

cells

for

of

equipped

was

for 20:4

by

standard.

of

Protein

(n-3)

(n-3),

Lipid

GC-14A)

CURCUMIN

incubation,

removed

in

internal

(0.25mm•~50m)

samples.

18:4 24-h

method

AND

20:4

sonicated

mentioned

as

(12).

or a

was the

or

metabolites.

method

(Shimadzu

paper

Student's

(n-6)

and

by

BY SESAMIN

(n-6)

After

suspension

(17:0)

column

Statistical cate

18:3

cell

the

GLC

previous

20:3

scraped

the

acid by

with

extracted

by

measured

with

saline, sonicated

HCl-methanol

METABOLISM

elongation.

phosphate-buffered aliquot

PUFA

5CP

detail

et al. at

least

in (16).

tripli

evaluated

by

t-test.

RESULTS

Metabolism

of

Table incubation were

n-6

with

n-3

the

concentrations

18:3

incubated from

(n-3),

any

the

of ‡™5-desaturation

Table

1.

Values script

Vol.

38,

good

Metabolism

were

No.

4,

model

are

1992

of

of

significantly

n-6

not

investigate

and

(n=4).

in

(n-3)

a (p