Technical Note
Endoreduplicative Standards for Calibration of Flow Cytometric C-Value Measurements David W. Galbraith*
School of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, Arizona Received 19 August 2013; Revised 5 December 2013; Accepted 27 December 2013 Grant sponsors: BIO5 Institute, the College of Agriculture and Life Sciences of the University of Arizona, and the National Institute of Food and Agriculture. Additional Supporting Information may be found in the online version of this article. *Correspondence to: David W. Galbraith; BIO5 Institute for Collaborative Bioresearch, 341 Keating Building, 1657 E. Helen Streey, Tucson, AZ 85721-0240 USA. E-mail [email protected] Published online 10 January 2014 in Wiley Online Library (wileyonlinelibrary. com) DOI: 10.1002/cyto.a.22440 C 2014 International Society for V
Abstract It has been estimated that there are, globally, as many as 400,000 species of the angiosperms (the flowering plants). Of these, a minimal proportion has been characterized at the cytological level. Urgency is required in initiating a systematic and comprehensive census, due to species extinction as a consequence of anthropogenic activities. Fundamental to eukaryotes is the 2C-value, the amount of DNA contained within the nucleus of the unreduced gametes. Flow cytometry provides an ideal method for determining C-values, but the values archived in the Kew Plant C-value Database represent
Endoreduplicative Standards for Calibration of Flow Cytometric C-Value Measurements David W. Galbraith*
School of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, Arizona Received 19 August 2013; Revised 5 December 2013; Accepted 27 December 2013 Grant sponsors: BIO5 Institute, the College of Agriculture and Life Sciences of the University of Arizona, and the National Institute of Food and Agriculture. Additional Supporting Information may be found in the online version of this article. *Correspondence to: David W. Galbraith; BIO5 Institute for Collaborative Bioresearch, 341 Keating Building, 1657 E. Helen Streey, Tucson, AZ 85721-0240 USA. E-mail [email protected] Published online 10 January 2014 in Wiley Online Library (wileyonlinelibrary. com) DOI: 10.1002/cyto.a.22440 C 2014 International Society for V
Abstract It has been estimated that there are, globally, as many as 400,000 species of the angiosperms (the flowering plants). Of these, a minimal proportion has been characterized at the cytological level. Urgency is required in initiating a systematic and comprehensive census, due to species extinction as a consequence of anthropogenic activities. Fundamental to eukaryotes is the 2C-value, the amount of DNA contained within the nucleus of the unreduced gametes. Flow cytometry provides an ideal method for determining C-values, but the values archived in the Kew Plant C-value Database represent
