Nucleic Acids Research, Vol. 18, No. 19 5895
Env gene of virus variant
new
transformation-defective Rous
Serge V.Zubak, Vladimir l.Kashuba, Vadim M.Kavsan, Alla V.Rynditch, Jan
sarcoma
Svobodal and
Ivo Hlozanek' Institute of Molecular Biology and Genetics, Acad. Sci.
Ukr. SSR, Zabolotnogo str.,
150, 252627
Kiev, USSR and 'Institute of Molecular Genetics, Acad. Sci., Flemingovo Czechoslovakia
nam.
Submitted on August 15, 1990
EMBL accession no. X51862
The recombinant clone (DFtd7) which contains nucleotide sequences of Rous sarcoma provirus was isolated from genomic DNA library of duck embryonic fibroblasts transformed with RSV adapted to these cells (1). Hybridization of DFtd7 with RSVspecific probes revealed that it contained gag, pol, env and LTR, but completely lost sequences of src gene. The deletion occurred on the boundaries of direct repeats (2) flanking src-gene from both sides in transforming RSV (3). This new transformationdefective variant of Rous sarcoma virus, Prague strain subgroup C (td da Pr-RSV-C) has 28 alterations in env-gene as compared with Pr-RSV-C sequenced previously (3). 19 alterations in td da Pr-RSV-C coincide with those discovered in the transforming duck-adapted variant of Rous sarcoma virus (da Pr-RSV-C) which are distributed in two clusters and are possible consequence of 1 61 121 181 241 301
TTCTTACAGA GGAACGTGCT TTATTTGGGT AGAAAGATGA AGCAAGAAGG CCCTTGCTGC
2, 16637 Prague,
homologous recombination with chicken endogenous RAV-0-sequences (4). The results may serve as an evidence that td da Pr-RSV-C was originated from transforming duck adapted Rous sarcoma virus variant.
REFERENCES 1. Kashuba,V.I., Zubak,S.V., Lazurkevitch,Z.V., Rynditch,A.V., Kavsan,V.M., Hlozanek,I. and Svoboda,J. (1989) Dokl. Akad. Nauk. SSSR.
304, 206-208. 2. Kashuba,V.I., Zubak,S.V., Rynditch,A.V., Kavsan,V.M., Hlozanek,I. and Svoboda,J. (1989) Nucl. Acids Res. 17, 2120. 3. Schwartz,D.E., Tizard,R. and Gilbert,W. (1983) Cell 32, 853-869. 4. Zubak,S.V., Rynditch,A.V., Kashuba,V.I., Kavsan,V.M. and Hlozanek,I., (1989) Nucd. Acids Res. 17, 6389.
GTTAAAATAC CGAGGTTATG AAAGTTAAAC CTTTTTGCAG GAAACCGCTA TAATTATATT I--> gp85 361 GTTACGGGGG TAAGAGCTGA TGTTCACTTA 421 TGGGCCAACC GTACAGGCCA AACGGATTTC 481 TTTCAAACAT GTTTGATAGG TATCCCGTCT 541 GTTTCTGATA CAAATTGCTC CACTGTGGGA 601 ACCGGTGGCC CTGACAACAG CACCACCCTC 661 AAGCTGAATG TCTCTATGTG GGATGAGCCA 721 CTCCCTAACG TTACTAACAT TACTCAGGTC 781 GCCCCAAGGG CCACTGGCCT GTTTTTAGGT 841 CTCCGTCACC CCTTTACCTC CACCTCTAAC 901 GATAGACACA ATCTTTTTAT GGGGAGTGAG 961 GAAATATATA ACTGCTCACA GACTAGGAAT 1021 GGCCTCCCTG AAACCTGGTG CAGAGGAAAA 1081 ATTAATGAGA CAGAGCCGTT CAGTTTTACT 1141 GTCAGCGGAT GTTGTGGAAA AACGACCACG 1201 ACACAAGGTA GTTTCACCAA ACCAAAAGCG 1261 GATCGCGCAT GGCAAGGAAT TCCCAGTCGT 1321 CTTACCATGT TAGCACCCAA CCATACAGAT 1--> gp37
GAATAGAGAC CCGCTGTGAA CGGACATCAC GCATTTCTGA GCAACAAGCA CTCATTATTG
AGGGGAGTGG AAACAGGGAC CCAAAAGGAT CTGGATACCC AGAAAGACCC GTGTCCTGGT
GAAAAAGGAT ACTGATAAGG GAGGTGACTA TGGGAAGACG GGAGAAGACA CTTGTGTGAG
CTCGAGCAGC TGCCTCTCTA CCTATTTCCG ACTGACCGGT ACTTATCGAA CCGGAACTAC TCTGGCGTGG TGGTCTAAAC TCCACGGAAC TACTGTGGTG ACTTACCGCT GGAGGTATAT GCGAACTGTA ATTCTCCCAT CTACCACCCG CCGGTAGGGG ATTCTCAAAA
CAGGGAACCT CACAGTCAGC AAGGTGATTT TAGTCTCGTC AAGTTTCATG AGCTGCTAGG CCGGGGGATG AAGGTCTCTC CGTTCACGGT CATATGGCTA
TTGGATTACA CACCTCCCCT TAAGGGATAT AGCCAGCATT
GTGGAGACGT GGGTTAATCA CAGCTAGTAA CAGGGGCGTG CAATTTTCCT GCCCCTGCTA
GGGAGGTACT
1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981
CACCTGGATG GCATCTATCT GCCTGTTGGT GATGTCACGA CTAGCTCACG
ATACATGCTC TAGCCCCGGG CCGTTAAACA GTATTCGACA GCCATGGCTG CTATACAGAA ACCCAATCGG TGCTAAAAGG GCCTTTTACA ATACTGAATA
AGATGAAGTG GGTAGCAGCT GGCTAACTTG CGCGGTCCTG TGAGGACGTT GAAGTTCCAG AAGTTGGCTG ACTGCTTTTG ATTTGTGTCT CAGGAAGATG
ACAGGTATAA CAGCTTTGGG GCGCAAGCCT ACAACATCAC CAGAACCGAG GCCGGAATGT CTAATGAAGA CGAGGGATAT GGGCTTGTAG AGTAGTATTC CAGGGCGGAG
AGGACCCCCG GGTCTGGGGA ACCCTCTCGA GGCGAGCCCT ACTCCAAGGA CAACGAGAGT
GACGTAAACG GTCCTACAGC TAAGAGAAAT TCCTCGGGGA CGGCTATTGA GTTGTTTCAA AACATGTCAA TCGGGGGAAT TTATTTTATT GAAAGATGAT CAGTCTAG
AAGCGTCTCA AAGAATCTTT TGAGAGACCA CTTATTGGAT CTTCTTGCTT TCTGAGTGAT TAAGATCGGC AGGGGAATGG GCTAGTAGTG TAATAGTTCA
CACAGTGAAT GTGGACAGCG GCCGTTCATC
TGCCTGCCCT ATCAACTATC
CTTGCTGTCA TTCCCAGTCT TGTATATTTC GCGGTTCCTC GGTGACAGCG CAGATTTTGG
ATCAAAGGAA TTTGGGTAAT GATCGACAGC CATTTGTGGG TTTAGGCAAG TACTTGCTAA TTCGTCGCGG
Env gene of virus variant
new
transformation-defective Rous
Serge V.Zubak, Vladimir l.Kashuba, Vadim M.Kavsan, Alla V.Rynditch, Jan
sarcoma
Svobodal and
Ivo Hlozanek' Institute of Molecular Biology and Genetics, Acad. Sci.
Ukr. SSR, Zabolotnogo str.,
150, 252627
Kiev, USSR and 'Institute of Molecular Genetics, Acad. Sci., Flemingovo Czechoslovakia
nam.
Submitted on August 15, 1990
EMBL accession no. X51862
The recombinant clone (DFtd7) which contains nucleotide sequences of Rous sarcoma provirus was isolated from genomic DNA library of duck embryonic fibroblasts transformed with RSV adapted to these cells (1). Hybridization of DFtd7 with RSVspecific probes revealed that it contained gag, pol, env and LTR, but completely lost sequences of src gene. The deletion occurred on the boundaries of direct repeats (2) flanking src-gene from both sides in transforming RSV (3). This new transformationdefective variant of Rous sarcoma virus, Prague strain subgroup C (td da Pr-RSV-C) has 28 alterations in env-gene as compared with Pr-RSV-C sequenced previously (3). 19 alterations in td da Pr-RSV-C coincide with those discovered in the transforming duck-adapted variant of Rous sarcoma virus (da Pr-RSV-C) which are distributed in two clusters and are possible consequence of 1 61 121 181 241 301
TTCTTACAGA GGAACGTGCT TTATTTGGGT AGAAAGATGA AGCAAGAAGG CCCTTGCTGC
2, 16637 Prague,
homologous recombination with chicken endogenous RAV-0-sequences (4). The results may serve as an evidence that td da Pr-RSV-C was originated from transforming duck adapted Rous sarcoma virus variant.
REFERENCES 1. Kashuba,V.I., Zubak,S.V., Lazurkevitch,Z.V., Rynditch,A.V., Kavsan,V.M., Hlozanek,I. and Svoboda,J. (1989) Dokl. Akad. Nauk. SSSR.
304, 206-208. 2. Kashuba,V.I., Zubak,S.V., Rynditch,A.V., Kavsan,V.M., Hlozanek,I. and Svoboda,J. (1989) Nucl. Acids Res. 17, 2120. 3. Schwartz,D.E., Tizard,R. and Gilbert,W. (1983) Cell 32, 853-869. 4. Zubak,S.V., Rynditch,A.V., Kashuba,V.I., Kavsan,V.M. and Hlozanek,I., (1989) Nucd. Acids Res. 17, 6389.
GTTAAAATAC CGAGGTTATG AAAGTTAAAC CTTTTTGCAG GAAACCGCTA TAATTATATT I--> gp85 361 GTTACGGGGG TAAGAGCTGA TGTTCACTTA 421 TGGGCCAACC GTACAGGCCA AACGGATTTC 481 TTTCAAACAT GTTTGATAGG TATCCCGTCT 541 GTTTCTGATA CAAATTGCTC CACTGTGGGA 601 ACCGGTGGCC CTGACAACAG CACCACCCTC 661 AAGCTGAATG TCTCTATGTG GGATGAGCCA 721 CTCCCTAACG TTACTAACAT TACTCAGGTC 781 GCCCCAAGGG CCACTGGCCT GTTTTTAGGT 841 CTCCGTCACC CCTTTACCTC CACCTCTAAC 901 GATAGACACA ATCTTTTTAT GGGGAGTGAG 961 GAAATATATA ACTGCTCACA GACTAGGAAT 1021 GGCCTCCCTG AAACCTGGTG CAGAGGAAAA 1081 ATTAATGAGA CAGAGCCGTT CAGTTTTACT 1141 GTCAGCGGAT GTTGTGGAAA AACGACCACG 1201 ACACAAGGTA GTTTCACCAA ACCAAAAGCG 1261 GATCGCGCAT GGCAAGGAAT TCCCAGTCGT 1321 CTTACCATGT TAGCACCCAA CCATACAGAT 1--> gp37
GAATAGAGAC CCGCTGTGAA CGGACATCAC GCATTTCTGA GCAACAAGCA CTCATTATTG
AGGGGAGTGG AAACAGGGAC CCAAAAGGAT CTGGATACCC AGAAAGACCC GTGTCCTGGT
GAAAAAGGAT ACTGATAAGG GAGGTGACTA TGGGAAGACG GGAGAAGACA CTTGTGTGAG
CTCGAGCAGC TGCCTCTCTA CCTATTTCCG ACTGACCGGT ACTTATCGAA CCGGAACTAC TCTGGCGTGG TGGTCTAAAC TCCACGGAAC TACTGTGGTG ACTTACCGCT GGAGGTATAT GCGAACTGTA ATTCTCCCAT CTACCACCCG CCGGTAGGGG ATTCTCAAAA
CAGGGAACCT CACAGTCAGC AAGGTGATTT TAGTCTCGTC AAGTTTCATG AGCTGCTAGG CCGGGGGATG AAGGTCTCTC CGTTCACGGT CATATGGCTA
TTGGATTACA CACCTCCCCT TAAGGGATAT AGCCAGCATT
GTGGAGACGT GGGTTAATCA CAGCTAGTAA CAGGGGCGTG CAATTTTCCT GCCCCTGCTA
GGGAGGTACT
1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981
CACCTGGATG GCATCTATCT GCCTGTTGGT GATGTCACGA CTAGCTCACG
ATACATGCTC TAGCCCCGGG CCGTTAAACA GTATTCGACA GCCATGGCTG CTATACAGAA ACCCAATCGG TGCTAAAAGG GCCTTTTACA ATACTGAATA
AGATGAAGTG GGTAGCAGCT GGCTAACTTG CGCGGTCCTG TGAGGACGTT GAAGTTCCAG AAGTTGGCTG ACTGCTTTTG ATTTGTGTCT CAGGAAGATG
ACAGGTATAA CAGCTTTGGG GCGCAAGCCT ACAACATCAC CAGAACCGAG GCCGGAATGT CTAATGAAGA CGAGGGATAT GGGCTTGTAG AGTAGTATTC CAGGGCGGAG
AGGACCCCCG GGTCTGGGGA ACCCTCTCGA GGCGAGCCCT ACTCCAAGGA CAACGAGAGT
GACGTAAACG GTCCTACAGC TAAGAGAAAT TCCTCGGGGA CGGCTATTGA GTTGTTTCAA AACATGTCAA TCGGGGGAAT TTATTTTATT GAAAGATGAT CAGTCTAG
AAGCGTCTCA AAGAATCTTT TGAGAGACCA CTTATTGGAT CTTCTTGCTT TCTGAGTGAT TAAGATCGGC AGGGGAATGG GCTAGTAGTG TAATAGTTCA
CACAGTGAAT GTGGACAGCG GCCGTTCATC
TGCCTGCCCT ATCAACTATC
CTTGCTGTCA TTCCCAGTCT TGTATATTTC GCGGTTCCTC GGTGACAGCG CAGATTTTGG
ATCAAAGGAA TTTGGGTAAT GATCGACAGC CATTTGTGGG TTTAGGCAAG TACTTGCTAA TTCGTCGCGG
