Life Sciences, Vol. 48, pp. 2457-2462 Printed in the U.S.A.
ACTIVITY AND DISTRIBUTION OF P H O S P H O L I P A S E IN HUMAN CARTILAGE.
M. Wloch and P. Vadas
I n f l a m m a t i o n Research Group, Departments of M e d i c i n e and Surgery, The W e l l e s l e y Hospital, University of Toronto, Ontario, Canada (Received in final form April 12, 1991) SUMMARY Extracellular phospholipase A2 (PLA2) with .proi n f l a m m a t o r y activity has recently been a l s c o v e r e a in synovial fluids in inflammatory arthritides. In the search for the sources of synovial fluid PLA2, human s y n o v i u m and articular cartilage were found to contain large q u a n t i t i e s of the enzyme. In rheumatoid arthritis (RA), PLA 2 activity in synovium, superficial and deep layers of articular cartilage was 20± 14 (SEM), 168± 62 and 533±176 nmol/min/mg protein respectively. Corresponding values in osteoarthritis (OA) were 49±11, 569±109 and 1709±243 nmol/min/mg protein, all s i g n i f i c a n t l y higher (p