Enzymatic synthesis of C, carotenes by cell-free preparation from
Can. J. Biochem. Downloaded from www.nrcresearchpress.com by STANFORD UNIV. on 11/19/14 For personal use only.
The Department of'Biocheanistty, University ofOttawa, Ottawa, Ont., Canada K1N6N5 AND
Tke Lipid Metabolism Labovrato~y,VefercrnsAdministrafion Hospital, and the Department ~fPhysiobgicwl C'kentistry, University of Wisconsin, Madison, WI, U.S.A.33706 Received January 30, 1976 Kushwaha, S. C., Kates, M. & Porter, J. W. (1976) Enzymatic synthesis of C4, carotenes by cell-free preparationifrom H~lohscreriumcubirubrum. Can. J . Biochern. 54, 816-4323 [I4CC]Mevalonateor [I4CC)opentenyl pyrophosphate was found to be converted to transphytoene, tk-ans-phytofluene, lycopene, and la-carotene by a cell-free 278 000 x g supernatant fraction prepared Rom Hulobncterium cutirifbrutn cells that were broken by manual grinding with glass beads. Incubations were done under N, in the dark at 37 OC in 4 M NaCl in presence of FAD, NADP, and MgCl,; ATP was also added when mevalonate was the substrate. This system was also capable of converting tmns-[I4C]phytoene to @-carotene via the intermediates trans-phytofluene,
Can. J. Biochem. Downloaded from www.nrcresearchpress.com by STANFORD UNIV. on 11/19/14 For personal use only.
The Department of'Biocheanistty, University ofOttawa, Ottawa, Ont., Canada K1N6N5 AND
Tke Lipid Metabolism Labovrato~y,VefercrnsAdministrafion Hospital, and the Department ~fPhysiobgicwl C'kentistry, University of Wisconsin, Madison, WI, U.S.A.33706 Received January 30, 1976 Kushwaha, S. C., Kates, M. & Porter, J. W. (1976) Enzymatic synthesis of C4, carotenes by cell-free preparationifrom H~lohscreriumcubirubrum. Can. J . Biochern. 54, 816-4323 [I4CC]Mevalonateor [I4CC)opentenyl pyrophosphate was found to be converted to transphytoene, tk-ans-phytofluene, lycopene, and la-carotene by a cell-free 278 000 x g supernatant fraction prepared Rom Hulobncterium cutirifbrutn cells that were broken by manual grinding with glass beads. Incubations were done under N, in the dark at 37 OC in 4 M NaCl in presence of FAD, NADP, and MgCl,; ATP was also added when mevalonate was the substrate. This system was also capable of converting tmns-[I4C]phytoene to @-carotene via the intermediates trans-phytofluene,
