Archives Internationales de Physiologie, de Biochimie et de Biophysique
ISSN: 0778-3124 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/iarp19
Erucic acid metabolism in rat liver a combined biochemical and radioautographical study C. Caselli, A. Bernard, J. Bezard & H. Carlier To cite this article: C. Caselli, A. Bernard, J. Bezard & H. Carlier (1992) Erucic acid metabolism in rat liver a combined biochemical and radioautographical study, Archives Internationales de Physiologie, de Biochimie et de Biophysique, 100:5, 309-320, DOI: 10.3109/13813459209000718 To link to this article: http://dx.doi.org/10.3109/13813459209000718
Published online: 26 Sep 2008.
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Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=iarp19 Download by: [University of California, San Diego]
Date: 04 April 2016, At: 23:44
Archives Innternationales de Physiologie, de Biochimie et de Biophysique, 1992, 100, 309-320
309 Requ Ie 2 juillet 1991
Erucic acid metabolism in rat liver A combined biochemical and radioautographical study ( * ) BY
C . CASELLI [(I)
(I),
A. BERNARD
(I),
J. BEZARD
(’) and
H . CARLIER
(I)
Deportement de Nutrition, ENS BANA, and ( I ) Unite de Recherches de Nutrition Cellulaire et Metobolique, Universite de Bourgogne, Dijon, France]
Downloaded by [University of California, San Diego] at 23:44 04 April 2016
(4 figures)
Metabolism of erucic acid was studied in rat liver in comparison with oleic acid in relation with diet lipids. Rats were fed for 3 or 60 days a balanced diet containing 30% of the calories of either rapeseed oil rich in erucic acid or sunflower seed oil rich in linoleic acid. They were intravenously injected with tritiated erucic or oleic acid. After 1 or 15 min, the radioactivity recovered in liver lipids was 9 to 26% whatever the diet or the acid injected. One minute after injection of erucic acid a high part of radioactivity was recovered in the free fatty acid fraction and as untransformed erucic acid. After 15 min the major part of radioactivity was recovered in the triacylglycerol fraction which contained a high proportion of labelled oleic acid formed by shortening of erucic acid. The autoradiography did not show any marked difference between the labelling of peroxisomes and mitochondria when tritiated erucic or oleic acid was injected. These data do not bring about arguments for a peroxisomal nor for a mitochondria1 location of erucic acid shortening in liver.
Key Words : erucic acid, liver, peroxisomes, mitochondria, radiochromatography, electron microscopy, radioautography .
Introduction
in any experiment, Apparently the ability to shorten erucic acid preesists in liver cells prior to any contact Regular ingestion of rapeseed oil, rich in erucic acid of the acid with the catabolyzing systems contrary to (cis-13-docosenoic acid), causes in a few days an acwhat is observed in heart cells. It has been well cumulation of erucic acid containing - triacylglycerols documented that erucic acid was very rapidly & VLES,1970; BEAREin rat myocardium (ABDELLATIF transformed into oleic acid in the liver (CARREAU et al., ROGERSet af., 1972; ROCQUELINet af., 1973; VLES, 1968; NORSETH & CHRISTOPHERSEN, 1972; BOUCROT & 1975; BEARE-ROGERS, 1977). The steatosis then disapBEZARD, 1973; ONG et al., 1977; BREMER & NORUM, pears and anatomical lesions develop in the myocar1982; CHRISTOPHERSEN et al., 1982; RONNEBERG et a[., dium (ROINEet af,, 1960; ABDELLATIF & VLES,1970; ROCQUELIN et a/., 1973; VLES,1975; BEARE-ROGERS, 1986). It is currentIy admitted that the shortening reaction 1977). The early accumulation of erucic acid in heart occurs in peroxisomes (LAZAROW & DE DUVE, 1976; is due to its poor oxidation in mitochondria (CARROLL, LAZAROW, 1978; BREMER & N O R U M , 1982; 1962; CLOUET et al., 1974; SWARTTOUW, 1974; BLOND et al., 1982; MASTERS& CRANE, et af.,1975; BREMER & NORUM, 1982; CHRISTOPHERSEN CHRISTOPHERSEN 1984). Increased erucic acid shortening is actually et al. 1982; NORMA”et al., 1983; BREMER & OSMUNDobserved when peroxisomal activity is stimulated by SEN, 1984) because of slow activation. To explain the high lipid-diets (CHRISTIANSEN et af.,1979; NEATet af., steatosis desappearance, it is generally admitted that 1981; BREMER & NORUM, 1982; CHRISTOPHERSEN et al., the presence of erucic acid in heart cells induces the 1982; OSMUNDSEN,1982; THOMASSEN et af., 1982; capability to shorten the very long chain-fatty acid, VEERKAMP & ZEVENBERGER, 1986; OSMUNDSEN et a)., generally to oleic acid, which is then easily oxidized by 1987). However erucic acid can also present a negative mitochondria ( PINSON& PADEU,1974; NORSETH, 1979; modulating effect on the peroxisomal oxidation (FLATNORSETHti a/., 1979; BREMER& NORUM,1982; MARK et al., 1983). On the other hand ingestion of CHRISTOPHERSEN el af., 1982). In liver, accumulation rapeseed oil (CLOUET et a/., 1976) or marine oil (CHRISof erucic acid rich-triacylglycerols was never observed (*) Preliminary results from this
work were presented at the “27th Inter-national Conference on the Biochemistry of Lipids”, 1986. Oslo, Norway.
310
C. CASELLI, A. BERNARD, J . BEZARD AND H. CARLJER
et al., 1981) increases the amount of mitochondria in heart or liver. Since mitochondria can also & BEZARD,1979; CLOUET shorten erucic acid (CLOUET et a f . , 1982) and since oxidative activity in liver mitochondria is much more elevated than in peroxisomes even when peroxisomal proliferation is induced (MANNAERTS et al., 1979; MANNAERTS & DEBEER, 1982), the question raises whether mitochondria could also participate to shortening of erucic acid in liver. Accordingly we have tried in this work to identify and to follow inside the hepatocytes the metabolic pathways of erucic acid in comparison with oleic acid which represents the major fatty acid formed by shortening or erucic acid. From the same experiment, results observed in heart cells and already published (CASELLI et al., 1990) showed that peroxisomes did not seem more implicated than mitochondria in erucic acid catabolism in the myocardium. Apparentely the observations reported here on liver cells confirm this point of view, but do not suggest any other interpretation.
Downloaded by [University of California, San Diego] at 23:44 04 April 2016
TIANSEN
Materials and Methods
* tr
Fatty Acids
Rapeseed oil
16:O 18:O 18:l n-9 18:2 n-6 18:3 n-3 20:o 20:l n-9 22:l n-9 24:l n-9
5.2 1 .5 12.0 17.4 8.4 6.8 41.7 I .o
=
Sunflower seed oil 1.5
4.0 23.5 64.5 -
ti-* tr* -
trace (
ISSN: 0778-3124 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/iarp19
Erucic acid metabolism in rat liver a combined biochemical and radioautographical study C. Caselli, A. Bernard, J. Bezard & H. Carlier To cite this article: C. Caselli, A. Bernard, J. Bezard & H. Carlier (1992) Erucic acid metabolism in rat liver a combined biochemical and radioautographical study, Archives Internationales de Physiologie, de Biochimie et de Biophysique, 100:5, 309-320, DOI: 10.3109/13813459209000718 To link to this article: http://dx.doi.org/10.3109/13813459209000718
Published online: 26 Sep 2008.
Submit your article to this journal
Article views: 1
View related articles
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=iarp19 Download by: [University of California, San Diego]
Date: 04 April 2016, At: 23:44
Archives Innternationales de Physiologie, de Biochimie et de Biophysique, 1992, 100, 309-320
309 Requ Ie 2 juillet 1991
Erucic acid metabolism in rat liver A combined biochemical and radioautographical study ( * ) BY
C . CASELLI [(I)
(I),
A. BERNARD
(I),
J. BEZARD
(’) and
H . CARLIER
(I)
Deportement de Nutrition, ENS BANA, and ( I ) Unite de Recherches de Nutrition Cellulaire et Metobolique, Universite de Bourgogne, Dijon, France]
Downloaded by [University of California, San Diego] at 23:44 04 April 2016
(4 figures)
Metabolism of erucic acid was studied in rat liver in comparison with oleic acid in relation with diet lipids. Rats were fed for 3 or 60 days a balanced diet containing 30% of the calories of either rapeseed oil rich in erucic acid or sunflower seed oil rich in linoleic acid. They were intravenously injected with tritiated erucic or oleic acid. After 1 or 15 min, the radioactivity recovered in liver lipids was 9 to 26% whatever the diet or the acid injected. One minute after injection of erucic acid a high part of radioactivity was recovered in the free fatty acid fraction and as untransformed erucic acid. After 15 min the major part of radioactivity was recovered in the triacylglycerol fraction which contained a high proportion of labelled oleic acid formed by shortening of erucic acid. The autoradiography did not show any marked difference between the labelling of peroxisomes and mitochondria when tritiated erucic or oleic acid was injected. These data do not bring about arguments for a peroxisomal nor for a mitochondria1 location of erucic acid shortening in liver.
Key Words : erucic acid, liver, peroxisomes, mitochondria, radiochromatography, electron microscopy, radioautography .
Introduction
in any experiment, Apparently the ability to shorten erucic acid preesists in liver cells prior to any contact Regular ingestion of rapeseed oil, rich in erucic acid of the acid with the catabolyzing systems contrary to (cis-13-docosenoic acid), causes in a few days an acwhat is observed in heart cells. It has been well cumulation of erucic acid containing - triacylglycerols documented that erucic acid was very rapidly & VLES,1970; BEAREin rat myocardium (ABDELLATIF transformed into oleic acid in the liver (CARREAU et al., ROGERSet af., 1972; ROCQUELINet af., 1973; VLES, 1968; NORSETH & CHRISTOPHERSEN, 1972; BOUCROT & 1975; BEARE-ROGERS, 1977). The steatosis then disapBEZARD, 1973; ONG et al., 1977; BREMER & NORUM, pears and anatomical lesions develop in the myocar1982; CHRISTOPHERSEN et al., 1982; RONNEBERG et a[., dium (ROINEet af,, 1960; ABDELLATIF & VLES,1970; ROCQUELIN et a/., 1973; VLES,1975; BEARE-ROGERS, 1986). It is currentIy admitted that the shortening reaction 1977). The early accumulation of erucic acid in heart occurs in peroxisomes (LAZAROW & DE DUVE, 1976; is due to its poor oxidation in mitochondria (CARROLL, LAZAROW, 1978; BREMER & N O R U M , 1982; 1962; CLOUET et al., 1974; SWARTTOUW, 1974; BLOND et al., 1982; MASTERS& CRANE, et af.,1975; BREMER & NORUM, 1982; CHRISTOPHERSEN CHRISTOPHERSEN 1984). Increased erucic acid shortening is actually et al. 1982; NORMA”et al., 1983; BREMER & OSMUNDobserved when peroxisomal activity is stimulated by SEN, 1984) because of slow activation. To explain the high lipid-diets (CHRISTIANSEN et af.,1979; NEATet af., steatosis desappearance, it is generally admitted that 1981; BREMER & NORUM, 1982; CHRISTOPHERSEN et al., the presence of erucic acid in heart cells induces the 1982; OSMUNDSEN,1982; THOMASSEN et af., 1982; capability to shorten the very long chain-fatty acid, VEERKAMP & ZEVENBERGER, 1986; OSMUNDSEN et a)., generally to oleic acid, which is then easily oxidized by 1987). However erucic acid can also present a negative mitochondria ( PINSON& PADEU,1974; NORSETH, 1979; modulating effect on the peroxisomal oxidation (FLATNORSETHti a/., 1979; BREMER& NORUM,1982; MARK et al., 1983). On the other hand ingestion of CHRISTOPHERSEN el af., 1982). In liver, accumulation rapeseed oil (CLOUET et a/., 1976) or marine oil (CHRISof erucic acid rich-triacylglycerols was never observed (*) Preliminary results from this
work were presented at the “27th Inter-national Conference on the Biochemistry of Lipids”, 1986. Oslo, Norway.
310
C. CASELLI, A. BERNARD, J . BEZARD AND H. CARLJER
et al., 1981) increases the amount of mitochondria in heart or liver. Since mitochondria can also & BEZARD,1979; CLOUET shorten erucic acid (CLOUET et a f . , 1982) and since oxidative activity in liver mitochondria is much more elevated than in peroxisomes even when peroxisomal proliferation is induced (MANNAERTS et al., 1979; MANNAERTS & DEBEER, 1982), the question raises whether mitochondria could also participate to shortening of erucic acid in liver. Accordingly we have tried in this work to identify and to follow inside the hepatocytes the metabolic pathways of erucic acid in comparison with oleic acid which represents the major fatty acid formed by shortening or erucic acid. From the same experiment, results observed in heart cells and already published (CASELLI et al., 1990) showed that peroxisomes did not seem more implicated than mitochondria in erucic acid catabolism in the myocardium. Apparentely the observations reported here on liver cells confirm this point of view, but do not suggest any other interpretation.
Downloaded by [University of California, San Diego] at 23:44 04 April 2016
TIANSEN
Materials and Methods
* tr
Fatty Acids
Rapeseed oil
16:O 18:O 18:l n-9 18:2 n-6 18:3 n-3 20:o 20:l n-9 22:l n-9 24:l n-9
5.2 1 .5 12.0 17.4 8.4 6.8 41.7 I .o
=
Sunflower seed oil 1.5
4.0 23.5 64.5 -
ti-* tr* -
trace (
