Environ Sci Pollut Res DOI 10.1007/s11356-014-3795-6
RESEARCH ARTICLE
Evidence for existence of quorum sensing in a bioaugmented system by acylated homoserine lactone-dependent quorum quenching Kun Zhang & Xin Zheng & Dong-sheng Shen & Mei-zhen Wang & Hua-jun Feng & Hong-zhen He & Shuo Wang & Jue-hua Wang
Received: 23 July 2014 / Accepted: 29 October 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The introduction of a gene, strain, or microbial consortium into an indigenous bacterial population is known as bioaugmentation. This technique has been proposed as an effective strategy for accelerating and enhancing the removal of recalcitrant and toxic compounds during wastewater treatment. In this study, three types of reactors were used to test whether quorum sensing plays an important role in bioaugmented systems. Reverse transcriptase polymerase chain reaction showed that the inoculated strain, HF-1, successfully colonized in the bioaugmented reactor. Meanwhile, no HF-1 colonization was observed in the quorum-quenching and non-bioaugmented reactors. Removal of nicotine in the bioaugmented reactor was almost 100 %, and removal of total organic carbon (TOC) was higher than 50 %. However, less than 20 % of nicotine and 30 % of TOC was removed in quorum-quenching and non-bioaugmented reactors. Moreover, the release of acylated homoserine lactones reached the threshold for HF-1 biofilm formation in bioaugmented reactors but not in quorum-quenching or non-bioaugmented reactors. The addition of porcine kidney acylase I, a quenching reagent, to the quorum-quenching reactor hampered the Kun Zhang and Xin Zheng contributed equally to this work. Responsible editor: Gerald Thouand K. Zhang : X. Zheng : D.
RESEARCH ARTICLE
Evidence for existence of quorum sensing in a bioaugmented system by acylated homoserine lactone-dependent quorum quenching Kun Zhang & Xin Zheng & Dong-sheng Shen & Mei-zhen Wang & Hua-jun Feng & Hong-zhen He & Shuo Wang & Jue-hua Wang
Received: 23 July 2014 / Accepted: 29 October 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The introduction of a gene, strain, or microbial consortium into an indigenous bacterial population is known as bioaugmentation. This technique has been proposed as an effective strategy for accelerating and enhancing the removal of recalcitrant and toxic compounds during wastewater treatment. In this study, three types of reactors were used to test whether quorum sensing plays an important role in bioaugmented systems. Reverse transcriptase polymerase chain reaction showed that the inoculated strain, HF-1, successfully colonized in the bioaugmented reactor. Meanwhile, no HF-1 colonization was observed in the quorum-quenching and non-bioaugmented reactors. Removal of nicotine in the bioaugmented reactor was almost 100 %, and removal of total organic carbon (TOC) was higher than 50 %. However, less than 20 % of nicotine and 30 % of TOC was removed in quorum-quenching and non-bioaugmented reactors. Moreover, the release of acylated homoserine lactones reached the threshold for HF-1 biofilm formation in bioaugmented reactors but not in quorum-quenching or non-bioaugmented reactors. The addition of porcine kidney acylase I, a quenching reagent, to the quorum-quenching reactor hampered the Kun Zhang and Xin Zheng contributed equally to this work. Responsible editor: Gerald Thouand K. Zhang : X. Zheng : D.
