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Examination of elongation factor Tu for aluminum fluoride binding sites using fluorescence and F-NMR methodologies Theodore L, HazlettI, Tsutomu Higashijlrnu~ and Dared M. Jameson t IDepctetmenl oJ Btorheml~ O" aad B#oph) ~ira. UttlveralO" of t l a . all at Manaa,/gdO F.o~t.H'¢~ Road, l~Ianolul., tht)~ali 9~$822 and D¢i~rtment v f Ph#rma¢ alog)'. University af Tr,xaa SouMwex/¢ra Medical Cemer. ~323 ttarr.t" Hm¢:: Bird,, Dollar, Texa~ ?.~235, USA Received 25 September 19~, revised ~,crsion received 15 November 1990 This art)tic reports on a companion of the tl,ller~clion t f AP' and F with two GTP.hindmg proteins, clonga;ion factor T- (~F-To} ~nd the humtune scnsllWC regulatory protein ((3 protein) O.z The racthodolo$1¢x tbotcn to elucidate i~ssihle interactions hot.ten protein and alumplum fluoride were Iluor¢~:en~e ~r~clro~:opy and nuclear raagn¢tl¢ r

Examination of elongation factor Tu for aluminum fluoride binding sites using fluorescence and 19F-NMR methodologies.

This article reports on a comparison of the interaction of Al3+ and F- with two GTP-binding proteins, elongation factor Tu (EF-Tu) and the hormone sen...
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