Appl Microbiol Biotechnol DOI 10.1007/s00253-014-6075-y
BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS
Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain Florian Lelchat & Joelle Cozien & Tinaïg Le Costaouec & Christophe Brandilly & Sophie Schmitt & Anne-Claire Baudoux & Sylvia Colliec-Jouault & Claire Boisset
Received: 21 May 2014 / Revised: 4 September 2014 / Accepted: 5 September 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they F. Lelchat : J. Cozien : C. Brandilly : S. Schmitt : S. Colliec-Jouault : C. Boisset IFREMER, Technopole Brest Iroise, 29280 Plouzané, France F. Lelchat e-mail: [email protected] J. Cozien e-mail: [email protected] C. Brandilly e-mail: [email protected] S. Schmitt e-mail: [email protected] S. Colliec-Jouault e-mail: [email protected] F. Lelchat : J. Cozien : C. Brandilly : S. Schmitt : S. Colliec-Jouault : C. Boisset IFREMER, Rue de l’Ile d’Yeu, 44311 Nantes cedex 3, France T. Le Costaouec : C. Boisset (*) CERMAV, 601 rue de la Chimie, 38049 Grenoble cedex 9, France e-mail: [email protected] T. Le Costaouec e-mail: [email protected] A.
BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS
Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain Florian Lelchat & Joelle Cozien & Tinaïg Le Costaouec & Christophe Brandilly & Sophie Schmitt & Anne-Claire Baudoux & Sylvia Colliec-Jouault & Claire Boisset
Received: 21 May 2014 / Revised: 4 September 2014 / Accepted: 5 September 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they F. Lelchat : J. Cozien : C. Brandilly : S. Schmitt : S. Colliec-Jouault : C. Boisset IFREMER, Technopole Brest Iroise, 29280 Plouzané, France F. Lelchat e-mail: [email protected] J. Cozien e-mail: [email protected] C. Brandilly e-mail: [email protected] S. Schmitt e-mail: [email protected] S. Colliec-Jouault e-mail: [email protected] F. Lelchat : J. Cozien : C. Brandilly : S. Schmitt : S. Colliec-Jouault : C. Boisset IFREMER, Rue de l’Ile d’Yeu, 44311 Nantes cedex 3, France T. Le Costaouec : C. Boisset (*) CERMAV, 601 rue de la Chimie, 38049 Grenoble cedex 9, France e-mail: [email protected] T. Le Costaouec e-mail: [email protected] A.
