Anal. Chem. 1992, 64, 1320-1338


Experimental Evaluation of the Separation Efficiency in Capillary Electrophoresis Using Open Tubular and Gel-Filled Columns Jinping Liu, Vladislav Dolnik,? You-Zung Hsieh, and Milos Novotny' Department of Chemistry, Indiana University, Bloomington, Indiana 47405

The band dlsperslon phenomena In caplllary zone electrophoresle (CZE) uslng untreated and surface-treated open tubular and gel-fllledcaplllarles were experhentaltyevaluated, wlth emphasis on small Capillary dlameters (10-100 pm). Laser-Induced fluorescence detectlon was used for hlghsensltlvlty detectlon of the Isolndoles orlglnated from model amlno aclds. The plotsof plate height vs electrlcfkld strength were generated for dlfferent column radll and compared wlth a theoretlcal model for CZE. I n addltlon to the dlffudoncontrolled band dispersion In the relatlvely low electrlc fleld range, adsorptive Interactions between a solute and the caplllary wall may play a certaln role In band-broadenlng.The sorptlon-dewrptbn klnetlcs becomeImportant wlth Increasing electrlc fleld strength. Thermal effects appear to contrlbute lmle to band-broadenlng In relatlvely small caplllarles ( G O pm 1.d.) wlthln normal operatlng voltages (7bpm 1.d.). Wlth gel-fllled capillaries of small dlameters (

Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns.

The band dispersion phenomena in capillary zone electrophoresis (CZE) using untreated and surface-treated open tubular and gel-filled capillaries were...
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