Experimental Vortex-Choroidal Angiograms Enoch N. C. Tsai, MD, J. Lawton Smith, MD
The choroidal circulation completely from the retinal circulation has not, to our knowledge, been previously demonstrated by fluorescein angiography. This has been accomplished in owl monkeys and rabbits by direct injection of fluorescein via a catheter introduced into a vortex vein by microsurgical technique. Morphologically distinct choroidal venous and arterial patterns were angiographically demonstrated in monkeys and rabbits. Some direct perfusion to the optic disc from the adjacent choroidal circulation was demonstrated in both species.
separated
of the choroidal cir¬ culation separated from the retinal circulation has not, to our knowledge, been previously seen by fluorescein angiography. It is now feasible to demonstrate the choroidal circulation by direct injec¬ tion of fluorescein into a vortex vein via a catheter placed in its lumen by
Demonstratiocompletely n
microsurgical technique.
Materials and Methods The experiments were carried out on ei¬ ther eye of six owl monkeys (Aötes trivirgatus) and ten rabbits. The monkeys were anesthetized with intramuscularly administered phencyclidine hydrochloride (Sernylen, 0.6-0.7 mg/lb of body weight) and intraperitoneally administered phéno¬ barbital sodium (Nembutal, 5 mg/lb). The rabbits were anesthetized with intraperi¬ toneally administered phénobarbital soSubmitted for publication June 14, 1973. From the Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Fla. Reprint requests to PO Box 520875, Biscayne Annex, Miami, FL 33152 (Dr. Smith).
dium (25 mg/lb). The pupils with atropine sulfate 1%.
were
dilated
Technique.—After anesthesia, the right femoral vein was catheterized with a poly¬ ethylene tube (PE No. 60) that was premeasured and marked so that its tip would be located at the proximal portion of the inferior vena cava. The femoral catheter was connected by a three-way stopcock to a 5-ml syringe containing heparinized sa¬ line and a 1-ml tuberculin syringe contain¬ ing 2% heparinized fluorescein. A small amount of heparinized saline was injected into the femoral catheter from time to time to prevent clotting. The intraocular pressure was measured at the beginning and end of most experi¬ ments by a Schi
The choroidal circulation completely from the retinal circulation has not, to our knowledge, been previously demonstrated by fluorescein angiography. This has been accomplished in owl monkeys and rabbits by direct injection of fluorescein via a catheter introduced into a vortex vein by microsurgical technique. Morphologically distinct choroidal venous and arterial patterns were angiographically demonstrated in monkeys and rabbits. Some direct perfusion to the optic disc from the adjacent choroidal circulation was demonstrated in both species.
separated
of the choroidal cir¬ culation separated from the retinal circulation has not, to our knowledge, been previously seen by fluorescein angiography. It is now feasible to demonstrate the choroidal circulation by direct injec¬ tion of fluorescein into a vortex vein via a catheter placed in its lumen by
Demonstratiocompletely n
microsurgical technique.
Materials and Methods The experiments were carried out on ei¬ ther eye of six owl monkeys (Aötes trivirgatus) and ten rabbits. The monkeys were anesthetized with intramuscularly administered phencyclidine hydrochloride (Sernylen, 0.6-0.7 mg/lb of body weight) and intraperitoneally administered phéno¬ barbital sodium (Nembutal, 5 mg/lb). The rabbits were anesthetized with intraperi¬ toneally administered phénobarbital soSubmitted for publication June 14, 1973. From the Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Fla. Reprint requests to PO Box 520875, Biscayne Annex, Miami, FL 33152 (Dr. Smith).
dium (25 mg/lb). The pupils with atropine sulfate 1%.
were
dilated
Technique.—After anesthesia, the right femoral vein was catheterized with a poly¬ ethylene tube (PE No. 60) that was premeasured and marked so that its tip would be located at the proximal portion of the inferior vena cava. The femoral catheter was connected by a three-way stopcock to a 5-ml syringe containing heparinized sa¬ line and a 1-ml tuberculin syringe contain¬ ing 2% heparinized fluorescein. A small amount of heparinized saline was injected into the femoral catheter from time to time to prevent clotting. The intraocular pressure was measured at the beginning and end of most experi¬ ments by a Schi
