J Huazhong Univ Sci Technol［Med Sci］ 10.1007/s11596-014-1353-6 34(5):785-790,2014 J DOI Huazhong Univ Sci Technol［Med Sci］ 34(5):2014
785
Expression and Implication of Toll-like Receptors TLR2, TLR4 and TLR9 in Colonic Mucosa of Patients with Ulcerative Colitis* Yan TAN (谭 琰)1, 2, Kai-fang ZOU (邹开芳)1#, Wei QIAN (钱 伟)1, Sheng CHEN (陈 胜)1, Xiao-hua HOU (侯晓华)1 1 Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Gastroenterology, Affiliated Hospital of Hainan Medical College, Haikou 570102, China © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: Toll-like receptors (TLRs) family may play important roles in inflammatory bowel disease. This study examined the expression of TLR2, TLR4 and TLR9 in the colonic tissues of patients with ulcerative colitis (UC) and explored their roles in the pathogenesis of UC. Colonic biopsies were taken from the colon of 30 patients with mild or moderate UC (at active phase) and 10 healthy controls during colonoscopy. TLR2, TLR4 and TLR9 protein expression levels were immunohistochemically detected. The mRNA expression levels of TLR2, TLR4 and TLR9 were assessed by reverse transcription polymerase chain reaction (RT-PCR). The disease activity index (DAI), colonoscopic and histologic grades and fecal microbial flora were determined. Histological examination showed that the intestinal mucous membrane of UC patients underwent acute inflammation changes. Immunohistochemistry exhibited that the expression levels of TLR2, TLR4 and TLR9 in colon epithelia and inflammatory cells were higher in UC patients than in control group (P
785
Expression and Implication of Toll-like Receptors TLR2, TLR4 and TLR9 in Colonic Mucosa of Patients with Ulcerative Colitis* Yan TAN (谭 琰)1, 2, Kai-fang ZOU (邹开芳)1#, Wei QIAN (钱 伟)1, Sheng CHEN (陈 胜)1, Xiao-hua HOU (侯晓华)1 1 Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Gastroenterology, Affiliated Hospital of Hainan Medical College, Haikou 570102, China © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: Toll-like receptors (TLRs) family may play important roles in inflammatory bowel disease. This study examined the expression of TLR2, TLR4 and TLR9 in the colonic tissues of patients with ulcerative colitis (UC) and explored their roles in the pathogenesis of UC. Colonic biopsies were taken from the colon of 30 patients with mild or moderate UC (at active phase) and 10 healthy controls during colonoscopy. TLR2, TLR4 and TLR9 protein expression levels were immunohistochemically detected. The mRNA expression levels of TLR2, TLR4 and TLR9 were assessed by reverse transcription polymerase chain reaction (RT-PCR). The disease activity index (DAI), colonoscopic and histologic grades and fecal microbial flora were determined. Histological examination showed that the intestinal mucous membrane of UC patients underwent acute inflammation changes. Immunohistochemistry exhibited that the expression levels of TLR2, TLR4 and TLR9 in colon epithelia and inflammatory cells were higher in UC patients than in control group (P
