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Original article
Expression of proliferation markers Ki67, cyclin A, geminin and aurora-kinase A in primary breast carcinomas and corresponding distant metastases Anna-Mária Tökés,1,2 A Marcell Szász,2 Franciska Geszti,2,3 Lilla V Lukács,4 István Kenessey,2 Eszter Turányi,5 Nóra Meggyesházi,5 István A Molnár,6 János Fillinger,7 Ibolya Soltész,7 Katalin Bálint,5,8 Zoltán Hanzély,8,9 Gabriella Arató,10 Miklós Szendröi,10 Janina Kulka2 ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ jclinpath-2014-202607). For numbered affiliations see end of article. Correspondence to Dr Anna-Mária Tökés, MTA-SE Tumor Progression Research Group, 2nd Department of Pathology, Semmelweis University, 93 Üllöi út, Budapest 1091, Hungary, [email protected] A-MT and AMS are equal first authors. Received 7 August 2014 Revised 1 December 2014 Accepted 19 December 2014 Published Online First 16 January 2015
ABSTRACT Aims To assess the expression of the following cell cycle regulatory proteins in primary metastatic breast carcinomas (MBCs) and on availability in matched distant metastases (DMs): Ki67, cyclin A, geminin and aurora-kinase A (aurkA); and to compare the expression of these markers in early MBC (EMBC) and late MBC separated into groups according to median time point on metastatic event occurred (28 months). Methods The expression of the above mentioned markers was analysed in a total of 47 primary MBCs and 59 DMs (out of which 37 were pairs) by immunohistochemistry. Fourteen breast carcinomas with no relapse over a 10-year follow-up period were utilised as control cases (CBC). Results Among the MBCs, 22 metastasised to the bone, 4 to the lung and 21 to the central nervous system (CNS). Geminin ( p
Original article
Expression of proliferation markers Ki67, cyclin A, geminin and aurora-kinase A in primary breast carcinomas and corresponding distant metastases Anna-Mária Tökés,1,2 A Marcell Szász,2 Franciska Geszti,2,3 Lilla V Lukács,4 István Kenessey,2 Eszter Turányi,5 Nóra Meggyesházi,5 István A Molnár,6 János Fillinger,7 Ibolya Soltész,7 Katalin Bálint,5,8 Zoltán Hanzély,8,9 Gabriella Arató,10 Miklós Szendröi,10 Janina Kulka2 ▸ Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/ jclinpath-2014-202607). For numbered affiliations see end of article. Correspondence to Dr Anna-Mária Tökés, MTA-SE Tumor Progression Research Group, 2nd Department of Pathology, Semmelweis University, 93 Üllöi út, Budapest 1091, Hungary, [email protected] A-MT and AMS are equal first authors. Received 7 August 2014 Revised 1 December 2014 Accepted 19 December 2014 Published Online First 16 January 2015
ABSTRACT Aims To assess the expression of the following cell cycle regulatory proteins in primary metastatic breast carcinomas (MBCs) and on availability in matched distant metastases (DMs): Ki67, cyclin A, geminin and aurora-kinase A (aurkA); and to compare the expression of these markers in early MBC (EMBC) and late MBC separated into groups according to median time point on metastatic event occurred (28 months). Methods The expression of the above mentioned markers was analysed in a total of 47 primary MBCs and 59 DMs (out of which 37 were pairs) by immunohistochemistry. Fourteen breast carcinomas with no relapse over a 10-year follow-up period were utilised as control cases (CBC). Results Among the MBCs, 22 metastasised to the bone, 4 to the lung and 21 to the central nervous system (CNS). Geminin ( p
