Journal of Medical Virology
Factors Affecting Sperm Fertilizing Capacity in Men Infected with HIV Dian Wang,1,2 Lianbing Li,3 Qingdong Xie,1 Zhiwei Hou,3 Xiaojun Yu,2 Mingfu Ma,3 and Tianhua Huang1* 1
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, China Department of Forensic Medicine, Shantou University Medical College, Shantou, China 3 Key Laboratory of Birth Defects and Reproductive Health of National Health and Family Planning Commission, Chongqing, China 2
Studies on the sperm-fertilizing capacity of HIV-seropositive men show conflicting results for reasons that are not yet clear. The aim of this study was to investigate the effects and relationships of some factors such as patient age, CD4þ cells count, fathering offspring, concomitant sexually transmitted diseases (STD), and receipt of highly active anti-retroviral therapy (HAART) on sperm fertilizing capacity. Semen samples were collected from 33 HIV-seropositive men. Data on the above factors were acquired from a self-designed questionnaire. Computer-assisted sperm analysis, a hypo-osmotic swelling, and zona-free hamster oocyte penetration tests were performed according to criteria of the World Health Organization. CD4þ cells in peripheral blood were examined using a flow cytometric (FCM) analyzer. Sperm vitality, sperm motility (grades a þ b), total sperm motility, and sperm penetration rates were significantly higher in patients whose CD4þ counts were 3350/ml than in those whose CD4þ counts were 0.05) (Table II).
Effects of Factors on Sperm Quality There was no significant difference in all semen parameters except sperm concentration between patients over and under the age of 30 (P > 0.05) (Table II). No significant effects on sperm tail swelling rate and sperm penetration rate were observed in the male patients regardless of whether they had or had not fathered offspring (Table II). Sperm vitality, sperm motility (grade a þ b), total sperm motility, and
Sperm Fertilizing Capacity is Affected by Total CD4þ Counts Sperm vitality, sperm motility (grades a þ b), total sperm motility, and sperm penetration rate were significantly correlated with CD4þ cell number (all P < 0.05). All semen parameters except sperm motility (grades a þ b), tail swelling rate, and sperm penetration rate were not correlated with the patient age J. Med. Virol. DOI 10.1002/jmv
4
Wang et al. þ
TABLE II. Dependence of Sperm Fertilizing Capacity on Patient Age, CD4 Counts, Fathering Offspring, Concomitant STD, and HAART CD4þ counts (/ml)
Age (years)
Factors Affecting Sperm Fertilizing Capacity in Men Infected with HIV Dian Wang,1,2 Lianbing Li,3 Qingdong Xie,1 Zhiwei Hou,3 Xiaojun Yu,2 Mingfu Ma,3 and Tianhua Huang1* 1
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, China Department of Forensic Medicine, Shantou University Medical College, Shantou, China 3 Key Laboratory of Birth Defects and Reproductive Health of National Health and Family Planning Commission, Chongqing, China 2
Studies on the sperm-fertilizing capacity of HIV-seropositive men show conflicting results for reasons that are not yet clear. The aim of this study was to investigate the effects and relationships of some factors such as patient age, CD4þ cells count, fathering offspring, concomitant sexually transmitted diseases (STD), and receipt of highly active anti-retroviral therapy (HAART) on sperm fertilizing capacity. Semen samples were collected from 33 HIV-seropositive men. Data on the above factors were acquired from a self-designed questionnaire. Computer-assisted sperm analysis, a hypo-osmotic swelling, and zona-free hamster oocyte penetration tests were performed according to criteria of the World Health Organization. CD4þ cells in peripheral blood were examined using a flow cytometric (FCM) analyzer. Sperm vitality, sperm motility (grades a þ b), total sperm motility, and sperm penetration rates were significantly higher in patients whose CD4þ counts were 3350/ml than in those whose CD4þ counts were 0.05) (Table II).
Effects of Factors on Sperm Quality There was no significant difference in all semen parameters except sperm concentration between patients over and under the age of 30 (P > 0.05) (Table II). No significant effects on sperm tail swelling rate and sperm penetration rate were observed in the male patients regardless of whether they had or had not fathered offspring (Table II). Sperm vitality, sperm motility (grade a þ b), total sperm motility, and
Sperm Fertilizing Capacity is Affected by Total CD4þ Counts Sperm vitality, sperm motility (grades a þ b), total sperm motility, and sperm penetration rate were significantly correlated with CD4þ cell number (all P < 0.05). All semen parameters except sperm motility (grades a þ b), tail swelling rate, and sperm penetration rate were not correlated with the patient age J. Med. Virol. DOI 10.1002/jmv
4
Wang et al. þ
TABLE II. Dependence of Sperm Fertilizing Capacity on Patient Age, CD4 Counts, Fathering Offspring, Concomitant STD, and HAART CD4þ counts (/ml)
Age (years)
