Fertilization and Mouse Embryo Development in the Presence of Midazolam R. James Swanson, PhD, and Maria G. Leavitt,

MS

Department of Biological Sciences, Old Dominion University, Norfolk, Virginia

Mouse embryo in vitro development elucidates the effect of a pharmacologic agent on cellular differentiation. Midazolam provides conscious sedation for patients undergoing egg retrieval for in vitro fertilization and is found in patient follicular fluid. Mouse preimplantation embryo formation and development were evaluated in the presence of midazolam. Midazolam was cocultured with two-cell mouse preimplantation embryos over 72 h and injected systemically just before ovulation and coitus. Concentrations to 12.5 @mL displayed no significant toxic effects on

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ouse embryo culturing was initiated in the Embryonics Laboratory at Old Dominion University in 1980 as a quality-control screening assay for media, supplements, culture ware, and related equipment used in developing the human in vitro fertilization program in Norfolk, Virginia (1-5). The mouse system was used to screen potential toxic effects of anesthetics used in egg retrieval for in vitro fertilization (IVF). Midazolam doses of 0.070-0.080 mg/kg were used for intravenous induction of anesthesia in IVF. We administered a total of approximately 0.07 mg/kg of midazolam to seven patients who were undergoing egg retrieval. Midazolam concentrations ranging from 17.7 to

Fertilization and mouse embryo development in the presence of midazolam.

Mouse embryo in vitro development elucidates the effect of a pharmacologic agent on cellular differentiation. Midazolam provides conscious sedation fo...
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