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FGF7 Over Expression Is an Independent Prognosticator in Patients with Urothelial Carcinoma of the Upper Urinary Tract and Bladder Eric W. Fan,* Ching-Chia Li,* Wen-Jeng Wu, Chun-Nung Huang, Wei-Ming Li, Hung-Lung Ke, Hsin-Chih Yeh, Ting-Feng Wu, Peir-In Liang, Li-Jung Ma† and Chien-Feng Li† From the Division of Urology, Department of Surgery (EWF) and Department of Pathology (LJM, CFL), Chi-Mei Medical Center, Department of Biotechnology, Southern Taiwan University of Science and Technology (TFW) and National Institute of Cancer Research, National Health Research Institutes (CFL), Tainan, Departments of Urology (CCL, WJW, CNH, WML, HLK, HCY) and Pathology (PIL), Kaohsiung Medical University Hospital, Graduate Institute of Medicine (CCL, WJW, WML, HLK, HCY) and Department of Urology, Faculty of Medicine (CCL, WJW, CNH, WML, HLK, HCY), College of Medicine, Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital (WJW) and Institute of Clinical Medicine (CFL), Kaohsiung Medical University and Department of Urology, Kaohsiung Municipal Ta-Tung Hospital (CCL, HCY), Kaohsiung and Pingtung Hospital, Ministry of Health and Welfare, Executive Yuan (WML), Pingtung, Taiwan, Republic of China
Purpose: Urothelial carcinoma of the bladder and upper tract is the most common tumor type in the urinary tract but its molecular pathogenesis and survival determinants remain obscure. By data mining a published transcriptomic database of bladder urothelial carcinoma (GSE31684) we identified FGF7 as the most significant gene up-regulated during urothelial carcinoma progression. We then used our well characterized urothelial carcinoma cohort to analyze FGF7 transcript and protein expression, and its clinicopathological significance. Materials and Methods: We performed real-time reverse transcriptasepolymerase chain reaction assay to determine the FGF7 transcript level in 30 fresh samples each of upper tract and bladder urothelial carcinoma. Immunohistochemistry evaluated by H-score was used to determine FGF7 protein expression in 340 upper tract and 295 bladder urothelial carcinomas. Transcript and protein expression were correlated with clinicopathological features. We further evaluated the prognostic significance of FGF7 protein expression for disease specific and metastasis-free survival. Results: An increased FGF7 transcript level was associated with higher pT stage in upper tract and bladder urothelial carcinoma (p ¼ 0.003 and
FGF7 Over Expression Is an Independent Prognosticator in Patients with Urothelial Carcinoma of the Upper Urinary Tract and Bladder Eric W. Fan,* Ching-Chia Li,* Wen-Jeng Wu, Chun-Nung Huang, Wei-Ming Li, Hung-Lung Ke, Hsin-Chih Yeh, Ting-Feng Wu, Peir-In Liang, Li-Jung Ma† and Chien-Feng Li† From the Division of Urology, Department of Surgery (EWF) and Department of Pathology (LJM, CFL), Chi-Mei Medical Center, Department of Biotechnology, Southern Taiwan University of Science and Technology (TFW) and National Institute of Cancer Research, National Health Research Institutes (CFL), Tainan, Departments of Urology (CCL, WJW, CNH, WML, HLK, HCY) and Pathology (PIL), Kaohsiung Medical University Hospital, Graduate Institute of Medicine (CCL, WJW, WML, HLK, HCY) and Department of Urology, Faculty of Medicine (CCL, WJW, CNH, WML, HLK, HCY), College of Medicine, Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital (WJW) and Institute of Clinical Medicine (CFL), Kaohsiung Medical University and Department of Urology, Kaohsiung Municipal Ta-Tung Hospital (CCL, HCY), Kaohsiung and Pingtung Hospital, Ministry of Health and Welfare, Executive Yuan (WML), Pingtung, Taiwan, Republic of China
Purpose: Urothelial carcinoma of the bladder and upper tract is the most common tumor type in the urinary tract but its molecular pathogenesis and survival determinants remain obscure. By data mining a published transcriptomic database of bladder urothelial carcinoma (GSE31684) we identified FGF7 as the most significant gene up-regulated during urothelial carcinoma progression. We then used our well characterized urothelial carcinoma cohort to analyze FGF7 transcript and protein expression, and its clinicopathological significance. Materials and Methods: We performed real-time reverse transcriptasepolymerase chain reaction assay to determine the FGF7 transcript level in 30 fresh samples each of upper tract and bladder urothelial carcinoma. Immunohistochemistry evaluated by H-score was used to determine FGF7 protein expression in 340 upper tract and 295 bladder urothelial carcinomas. Transcript and protein expression were correlated with clinicopathological features. We further evaluated the prognostic significance of FGF7 protein expression for disease specific and metastasis-free survival. Results: An increased FGF7 transcript level was associated with higher pT stage in upper tract and bladder urothelial carcinoma (p ¼ 0.003 and
