521

TKANS.WXIONSOF THE ROYAL SOCIETYOF TROPICALMEDICINEAND HYGIENE (1990) 84, 521-525

Field evaluation of the use of an ELISA to detect chloroquine metabolites in blood, urine and breast-milk

and its

A. M. C. Witte’, H. J. H. Klever’, B. J. Brabin2p4, T. A. Eggeite3, H. J. Van der Kaay’ and M. P. Alpers’ ‘Laboratoy of Parasitology, Institute of Tropical Medicine, University of Leiden, Post Box 9605,230O RC Leiden, The Netherlands; 2Papua New Guinea Institute of Medical Research, P.O. Box 378, Madang, Papua New Guinea; 3Royal Tropical Insitute, N. H. Swellengrebel Laboratory for Tropical Hygiene, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands; 4Department of Tropical Paediatrics and International Child Health, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 SQA, UK

Abstract The evaluation of an enzyme-linked immunosorbent assav (ELISA) for chloroauine and its metabolites in b&d, urine and breast-milk is reported. ELISA blood levels, following standard treatment with chloroquine of pregnant and non-pregnant women, showed mean values comparable to other analytical methods. Blood chloroquine concentrations were estimated at day 0, 35tiOO nglml; day 2, 1000-1500 ne/ml: dav 14. 350-400 r&ml; dav 28, 180-350 ng/&l. In a