Childs Nerv Syst DOI 10.1007/s00381-014-2415-6
ORIGINAL PAPER
Fingerprint changes in CSF composition associated with different aetiologies in human neonatal hydrocephalus: inflammatory cytokines Irum Naureen & Kh. A. Irfan Waheed & Ahsen W. Rathore & Suresh Victor & Conor Mallucci & John R. Goodden & Shahid N Chohan & Jaleel A. Miyan
Received: 23 January 2014 / Accepted: 27 March 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Purpose Hydrocephalus (HC) has a multifactorial and complex picture of pathophysiology due to aetiology, age at and duration since onset. We have previously identified distinctions in markers of cell death associated with different aetiologies. Here, we examined cerebrospinal fluid (CSF) from human HC neonates for cytokines to identify further distinguishing features of different aetiologies. Methods CSF was collected during routine lumbar puncture or ventricular tap from neonates with hydrocephalus, or with no neurological condition (normal controls). Total protein, Fas receptor, Fas ligand, stem cell factor (SCF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin growth factor-1 (IGF-1), tumour necrosis I. Naureen : J. A. Miyan (*) Faculty of Life Sciences, The University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK e-mail: [email protected] K. A. I. Waheed : A. W. Rathore Department of Neonatology, The Children’s Hospital and Institute of Child Health, Ferozepur Road, Lahore, Pakistan S. Victor Institute of Human Development, Manchester Academic Health Sciences Centre, The University of Manchester, Oxford Road, Manchester M13 9WL, UK C. Mallucci Neurosurgical Unit, Alder Hey Children’s Hospital, Eaton Road, Liverpool L12 2AP, UK J. R. Goodden Department of Neurosurgery, Leeds General Infirmary, Great George Street, Leeds LS1 3EX, UK I. Naureen : S. N. Chohan Department of Biosciences, COMSATS Institute of Information and Technology, Islamabad, Pakistan
factor alpha (TNF-α) and interleukin 6 (IL-6) were measured and compared between 8 unaffected and 28 HC neonatal CSF samples. Results Total protein was significantly (P
ORIGINAL PAPER
Fingerprint changes in CSF composition associated with different aetiologies in human neonatal hydrocephalus: inflammatory cytokines Irum Naureen & Kh. A. Irfan Waheed & Ahsen W. Rathore & Suresh Victor & Conor Mallucci & John R. Goodden & Shahid N Chohan & Jaleel A. Miyan
Received: 23 January 2014 / Accepted: 27 March 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Purpose Hydrocephalus (HC) has a multifactorial and complex picture of pathophysiology due to aetiology, age at and duration since onset. We have previously identified distinctions in markers of cell death associated with different aetiologies. Here, we examined cerebrospinal fluid (CSF) from human HC neonates for cytokines to identify further distinguishing features of different aetiologies. Methods CSF was collected during routine lumbar puncture or ventricular tap from neonates with hydrocephalus, or with no neurological condition (normal controls). Total protein, Fas receptor, Fas ligand, stem cell factor (SCF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin growth factor-1 (IGF-1), tumour necrosis I. Naureen : J. A. Miyan (*) Faculty of Life Sciences, The University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK e-mail: [email protected] K. A. I. Waheed : A. W. Rathore Department of Neonatology, The Children’s Hospital and Institute of Child Health, Ferozepur Road, Lahore, Pakistan S. Victor Institute of Human Development, Manchester Academic Health Sciences Centre, The University of Manchester, Oxford Road, Manchester M13 9WL, UK C. Mallucci Neurosurgical Unit, Alder Hey Children’s Hospital, Eaton Road, Liverpool L12 2AP, UK J. R. Goodden Department of Neurosurgery, Leeds General Infirmary, Great George Street, Leeds LS1 3EX, UK I. Naureen : S. N. Chohan Department of Biosciences, COMSATS Institute of Information and Technology, Islamabad, Pakistan
factor alpha (TNF-α) and interleukin 6 (IL-6) were measured and compared between 8 unaffected and 28 HC neonatal CSF samples. Results Total protein was significantly (P
