1993
xanthene dyes
xanthene dyes T 1000 Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. 02 - 246 — 5-Carboxyfluorescein (I) is activated, cyclized, and finally coupled with the aminopropoxymethyldioxolane (III), forming the adduct (IV). Subsequent hydrolysis, dimethoxytritylation, and phosphitylation afford the 5-carboxyfluorescein phosphoramidite (VII). This is used in DNA synthesizers to prepare directly fluorescent dye labelled oligonucleotides (FDLO) by coupling to the 5’-hydroxylic function of the support bounded oligonucleotide. Furthermore, a polymeric support ( e.g. polystyrene) can be treated with (VII) to prepare 3’-fluorescent labelled oligonucleotides. — (THEISEN, P.; MCCOLLUM, C.; UPADHYA, K.; JACOBSON, K.; VU, H.; ANDRUS, A.; Tetrahedron Lett. 33 (1992) 35, 5033-5036; Applied Biosystems Inc., Foster City, CA 94404, USA; EN)
1
xanthene dyes
xanthene dyes T 1000 Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. 02 - 246 — 5-Carboxyfluorescein (I) is activated, cyclized, and finally coupled with the aminopropoxymethyldioxolane (III), forming the adduct (IV). Subsequent hydrolysis, dimethoxytritylation, and phosphitylation afford the 5-carboxyfluorescein phosphoramidite (VII). This is used in DNA synthesizers to prepare directly fluorescent dye labelled oligonucleotides (FDLO) by coupling to the 5’-hydroxylic function of the support bounded oligonucleotide. Furthermore, a polymeric support ( e.g. polystyrene) can be treated with (VII) to prepare 3’-fluorescent labelled oligonucleotides. — (THEISEN, P.; MCCOLLUM, C.; UPADHYA, K.; JACOBSON, K.; VU, H.; ANDRUS, A.; Tetrahedron Lett. 33 (1992) 35, 5033-5036; Applied Biosystems Inc., Foster City, CA 94404, USA; EN)
1
