J Mol Med DOI 10.1007/s00109-013-1119-9
ORIGINAL ARTICLE
FP-receptor gene silencing ameliorates myocardial fibrosis and protects from diabetic cardiomyopathy Wen-yuan Ding & Lin Liu & Zhi-hao Wang & Meng-xiong Tang & Yun Ti & Lu Han & Lei Zhang & Yun Zhang & Ming Zhong & Wei Zhang
Received: 8 August 2013 / Revised: 2 December 2013 / Accepted: 6 December 2013 # Springer-Verlag Berlin Heidelberg 2014
Abstract Prostaglandin F2α-F-prostanoid (PGF2α-FP) receptor is closely related to insulin resistance, which plays a causal role in the pathogenesis of diabetic cardiomyopathy (DCM). We sought to reveal whether PGF2α-FP receptor plays an important part in modulating DCM and the mechanisms involved. We established the type 2 diabetes rat model by high-fat diet and low-dose streptozotocin (STZ) and then evaluated its characteristics by metabolite tests, Western blot analysis for FP-receptor expression, histopathologic analyses of cardiomyocyte density and fibrosis area. Next, we used gene silencing to investigate the role of FP receptor in the pathophysiologic features of DCM. Our study showed elevated cholesterElectronic supplementary material The online version of this article (doi:10.1007/s00109-013-1119-9) contains supplementary material, which is available to authorized users. W.
ORIGINAL ARTICLE
FP-receptor gene silencing ameliorates myocardial fibrosis and protects from diabetic cardiomyopathy Wen-yuan Ding & Lin Liu & Zhi-hao Wang & Meng-xiong Tang & Yun Ti & Lu Han & Lei Zhang & Yun Zhang & Ming Zhong & Wei Zhang
Received: 8 August 2013 / Revised: 2 December 2013 / Accepted: 6 December 2013 # Springer-Verlag Berlin Heidelberg 2014
Abstract Prostaglandin F2α-F-prostanoid (PGF2α-FP) receptor is closely related to insulin resistance, which plays a causal role in the pathogenesis of diabetic cardiomyopathy (DCM). We sought to reveal whether PGF2α-FP receptor plays an important part in modulating DCM and the mechanisms involved. We established the type 2 diabetes rat model by high-fat diet and low-dose streptozotocin (STZ) and then evaluated its characteristics by metabolite tests, Western blot analysis for FP-receptor expression, histopathologic analyses of cardiomyocyte density and fibrosis area. Next, we used gene silencing to investigate the role of FP receptor in the pathophysiologic features of DCM. Our study showed elevated cholesterElectronic supplementary material The online version of this article (doi:10.1007/s00109-013-1119-9) contains supplementary material, which is available to authorized users. W.
