DEVELOPMENTAL
BIOLOGY
145, 203-204
(1991)
ERRATA Volume 142, Number 2 (1990), in the article “Expression of Growth Factors during the Differentiation of Embryonic Stem Cells in Monolayer,” by C. L. Mummery, A. J. M. van den Eijnden-van Raaij, A. Feijen, E. Freund, E. Hulskotte, J. Schoorlemmer, and W. Kruijer, pages 406-413: On page 408, lines 7 and 8 under Probes for Northern Blot Analysis should read “TGFP, (chicken): 1.4-kb fragment of pTGFP-ChX 63 cloned into pUC19 (Jakowlew et al., 198813)” instead of “TGFP, (chicken): 2%kb fragment of PTGFB-Ch X 1’7 cloned into pUC19 (Jakowlew et aZ., 1988b).”
Volume 142, Number 2 (1990), in the article “Genomic Organization and Polypeptide Primary Structure of Zona Pellucida Glycoprotein hZP3, the Hamster Sperm Receptor,” by Ross A. Kinloch, Betina Ruiz-Seiler, and Paul M. Wassarman, pages 414-421: A few errors were introduced into the hZP3 mRNA and polypeptide primary structures during preparation of Figs. 3 and 4. The errors were introduced during transfer of sequence information from computer outputs into a word processor. Five codons and two amino acids have been changed in the revised version of Fig. 3 (see following page) and one amino acid has been changed in the revised version of Fig. 4 (see Column 2). The correct figures and amended legends are reprinted here for the reader’s convenience.
M MAsSYFLFLI;LLLI;GGPE~~~SQTLWLLPG H .GL..Q.L....... .AKQ.C..P......
30 30
M GTPTPVGSSSPVKVEGLEAELWTVSRDLF H . . . ..GKLT.S.E.................
60 60
M GTGKLVQPGDLTLGSEGCQPRVSVDTDWR H . . . ..I..E.......N.R.L...A.....
90 90
M FNAQLHECSSRVQMTKDALVYSTFLLHDPR H .K.......N...V.E.......V...Q..
120 120
M PVSGLSILRTNRVEVPIE&RYPRQGmSSH H ..P.........AD................
150 150
M PIQPTWVPFRATVSSEEKLAFSLRLMEENW H A.R......ST........V..........
180 180
M NTEKSAPTFHLGEVAHLQAEVQTGSHLPLQ H . . . LS.........Y.............L
210 210
M LFVDHCVATPSPLPDPNSSPYHFIVDFHG& H . . ..R.......--.QTA....V.......
240 238
M LVDGLSESFSAFQVPRPRPETLQFTVDVFH 270 H . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 M FA@SSRNTLYITCHLKVAPANQIPDKLNKA H . , . . . . . . I........T..,.T..E....
300 298
M CSFNKTSQSWLPVEGDADICDCCSHGNCSN H . . ..RS.K..S......EV.G...S.D.GS
330 328
M SSSSQFQIHGPRQWSKLVSRNRRHVTDEAD H ..R.RY.A..VS..P.SA..R....R....
360 358
M VTVGPLIFLGKANDQTVEGWTASAQTSVAL H . . . . . . . . . . ..S..A....AS.....L..
390 388
M GLGLATVAFLTLAAIVLAVTRKCHSSSSYLV H . . . ..A...........G...S..TP.HV.
420 418
M SLPQ 424 H ..S. 422 FIG. 4. Comparison of primary structures of mZP3 and hZP3 polypeptides. The single-letter amino acid code is used. Mouse (designated M) and hamster (designated H) polypeptides are aligned so as to provide maximum similarity between the polypeptides. The entire mZP3 amino acid sequence is shown. For hZP3, only those positions where the amino acid sequence differs from mZP3 are shown in the singleletter code. A two-amino acid deletion in hZP3 is indicated by dashes at positions 223 and 224 of mZP3. The predicted signal sequences are italicized (residues l-22), and locations of cysteine residues (C) and potential N-linked glycosylation sites (N) are underlined. [Here, hZP3 amino acid 303 is R.]
203
204
DEVELOPMENTALBIOLOGY
VOLUME 145,1991 5'-ATGCAGCGGGCCTTATTCAGGCAGTACC
ATG MET 1 TGT CYS 21 TCC SER 41 GGC GLY 61 CTG LEU 81
GGG CTG AGC GLY LEU SER
TAC CAG TYR GLN
TCC CAG SER GLN
CTG TGG CTC LEU TRP LEU
CCT PRO
CTC LEU
CTC LElJ
CTG TGT CTC CTG CTG TGT GGA LEU CYS LEIJ LEU LEV CYS GLY
TTG CCA LEU PRO
GGC GGA ACT GLY GLY THR
CCG ACC PRO THR
GTG GAG GTG GAG TGT CTG GAA VAL GLU VAL GLU cys LEU GLU
GCT GAG CTC GTG GTG ACT ALA GLU LEU VAL VAL THR
ACC THR
GAG GAC GLU ASP
GGG AAG GLY LYS
GTT TCC VAL SER
CTC ATA LEU ILE
GTG GCT ACG VAL ALA THR
CAG GLN
CCC PRO
CTC ACC CTT LEU THR LEU
GAT GTG GTC AGG TTC AAG ASP VAL VAL ARG PHE LYS
GCC ALA
CCA PRO
GGC GCC GLY ALA
AAG LYS
GGA AAG GLY LYS
CTC LEU
GTC AGT VAL SER
AGA ARG
GAC Asp
GGC TCA GLY SER
GAA GLU
AAC ASN
TGT CYS
CAG GLN
CAT HIS
GAA GLU
TGC CYS
TTG LEU
CAG GLN
TGC CYS 20 ACG TCA THR SER 40 CTT TTT LEu PHE 60 CGG CCC ARG PRO 80 AGC Aii SER ASN 100
AGG GTG CAG GTG ACG GAA GAT GCC CTG GTG TAC AGC ACC GTG CTG CTG CAC CAA CCC CGC
ARG 101 CCT PRO 121 TAC TYR 141 ACC THR 161 AAC ASN 181
VAL
GLN
GTG CCC VAL PRO
VAL
THR GLU
ASP
ALA
VAL
TYR
SER
GGC CTG TCC GLY LEU SER
ATC ILE
CTG AGG ACT LEU ARG THR
AAC ASN
CGT GCG GAC ARG ALA ASP
CCC PRO
AGG CAG GGC AAT ARG GLN GLY ASN
ACT THR
GTG TCC VAL SER
TCA SER
ACT THR
GAG AAA GLU LYS
TTG TCA LEU SER
GTG AGC VBL SEB
GAG GAG AAG GLU GLU LYS CCC PRO
ACC THR
LEU
AGC SER
CAC HIS
GCT ATC ALA ILE
CTG GTT TTC LEU VAL PHE TCC SER
CAC HIS
TCT SER
THR
VAL
LEU
LEU
GTG VAL
CCT PRO
CGG CCC ARG PRO
ACC THR
TGG TRP
CTC LEU
CTG ATG LEU MET
CGC ARG
CTG GGA GAG GTA LEU GLY GLU VAL
GCC ALA
TAC TYR
HIS
GLN
PRO
ARG 120 ATT GAG TGC CGC ILE GLU CYS ARG 140 GTT CCC TTC AGC VAL PRO PHE SER 160 GAG GAG AAC TGG GLU GLU ASN TRP 180 CTC CAG GCA GAG LEU GLN ALA GLU 7nn
GTC CAG ACT GGA AGC CAT CTG CCA CTG CTG CTG TTT GTG GAC CGC TGT GTG CCC ACA :CT
VAL 201 TCG SER 221 GAT ASP 241 CAG GLN 261 TGT CYS 281 TTC PHE 301 TGC CYS 321 CAG GLN 341 GTA VAL 361 TCT SER 381 GcT ALA 401 TCA SER 421
GLN
THR
GLY
SER
HIS
LEU
PRO
LEU
LEU
LEU
CCG PRO
GAC ASP
CAG GLN
ACC THR
GCC ALA
TCT SER
CCC PRO
TAT TYR
CAT HIS
GTC ATT VAL ILE
GGT CTA GLY LEU
TCT SER
GAG AGC GLU SER
TTT TCT PHE SER
GCA ALA
TTT CAA PHE GLN
TTC PHE
GTG GAT GTA VAL ASP VAL
TTC PHE
CAT HIS
TTT GCC PHE ALA
ACG THR
VAL
ASP
ARG
CYS
VAL
ALA
GTG GAC VAL ASP
TTC PHE
CAT HIS
GGT GLY
TGC CTT CYS LEU
GTG CCT AGA VAL PRO ARG
CCC PRO
CGG CCG ARG PRO
GAG ACT GLU THR
AAT BSN
AGC
TCC
AAT ASN
ACG THR
ATC ILE
TAT TYR
ACC
CCA PRO
GAT GAG CTC ASP GLU LEU
AAC ASN
AAA LYS
AGA ARG
CAT CTC AAA HIS LEU LYS
GTC ACC VAL THR
CCA PRO
GCC AAC ALA BSN
AAC &SN
AGG TCT ARG SEB
TCC SER
AGT SER
TGG TCG CCA GTA TRP SER PRO VAL
AGC SER
AGT SER
GGC GLY
GAC TGT GGT ASP CYS GLY
AGC SER
TCA SER
TGG CCC TRP PRO
AAG LYS
TCG GCA SER ALA
TCT SER
AGA ARG
CGC CGC AGG CAC ARG ARG ARG HIS
GGA CCC GLY PRO
CTG ATC LEU ILE
TTC PHE
CTG LEU
GGA AAG GCA GLY LYS ALA
AGC SER
GCT CAA ALA GLN
ACC THR
TTG GCT LEU ALA
CTT GGT TTA LEU GLY LEU
GGC CTA GCC GCA GTG GCA GLY LEU ALA ALA VAL ALA
TTC PHE
GCT ATT ALA ILE
GTC CTC VAL LEU
TGT CAC CYS HIS
GTT VAL
TCT SER
AAC LYS
CAG
PHE
AGC SER
GGT GTC ACC AGG AGT GLY VAL THR ARG SER
GAG GGC GAT GLU GLY ASP
GCT ALA
GAG GTC GLU VAL
CGT TCA CGG TAC ARG SER ARG TYR
CAG GLN
GCC ALA
CAT HIS
GTG AGA GAC VAL ARG ASP
GAA GLU
GCT ALA
GAC ASP
CAG GLN
ACC THR
GCT GTG GAG GGC ALA VAL GLU GLY
CCT PRO
TCC SER
CAT HIS
THR
ATC ILE
PRO 220 GTG VAL 240 CTT LEU 260 ACC THR
280 TGC TCC CYS SER 300 TGC GGC TGC CYS GLY Cys 320 GGA GTG AGC GLY VAL SER 340 GAT GTC ACG ASP VAL THR 360 TGG GCC TCT TRP ALA SER 380 CTG ACC CTG LEU THR LE" 400 GTA TCC CTT VAL SER LEIJ 420 GCC ALA
CAATAAAAAGTCCAGTTCTG-3' GLN
FIG. 3. Primary structure of hZP3 mRNA (top) locations of potential N-linked glycosylation respectively, and the codons for the following (AGC), and Asp-214 (GAC).]
and polypeptide (bottom). The predicted signal sequence (residues l-22) is italicized and sites (Asn-X-Ser/Thr) are underlined. [Here, hZP3 amino acids 251 and 303 are Gln and Arg, five amino acids have been corrected: Thr-54 (ACT), Glu-138 (GAG), Phe-159 (TTC), Ser-160
BIOLOGY
145, 203-204
(1991)
ERRATA Volume 142, Number 2 (1990), in the article “Expression of Growth Factors during the Differentiation of Embryonic Stem Cells in Monolayer,” by C. L. Mummery, A. J. M. van den Eijnden-van Raaij, A. Feijen, E. Freund, E. Hulskotte, J. Schoorlemmer, and W. Kruijer, pages 406-413: On page 408, lines 7 and 8 under Probes for Northern Blot Analysis should read “TGFP, (chicken): 1.4-kb fragment of pTGFP-ChX 63 cloned into pUC19 (Jakowlew et al., 198813)” instead of “TGFP, (chicken): 2%kb fragment of PTGFB-Ch X 1’7 cloned into pUC19 (Jakowlew et aZ., 1988b).”
Volume 142, Number 2 (1990), in the article “Genomic Organization and Polypeptide Primary Structure of Zona Pellucida Glycoprotein hZP3, the Hamster Sperm Receptor,” by Ross A. Kinloch, Betina Ruiz-Seiler, and Paul M. Wassarman, pages 414-421: A few errors were introduced into the hZP3 mRNA and polypeptide primary structures during preparation of Figs. 3 and 4. The errors were introduced during transfer of sequence information from computer outputs into a word processor. Five codons and two amino acids have been changed in the revised version of Fig. 3 (see following page) and one amino acid has been changed in the revised version of Fig. 4 (see Column 2). The correct figures and amended legends are reprinted here for the reader’s convenience.
M MAsSYFLFLI;LLLI;GGPE~~~SQTLWLLPG H .GL..Q.L....... .AKQ.C..P......
30 30
M GTPTPVGSSSPVKVEGLEAELWTVSRDLF H . . . ..GKLT.S.E.................
60 60
M GTGKLVQPGDLTLGSEGCQPRVSVDTDWR H . . . ..I..E.......N.R.L...A.....
90 90
M FNAQLHECSSRVQMTKDALVYSTFLLHDPR H .K.......N...V.E.......V...Q..
120 120
M PVSGLSILRTNRVEVPIE&RYPRQGmSSH H ..P.........AD................
150 150
M PIQPTWVPFRATVSSEEKLAFSLRLMEENW H A.R......ST........V..........
180 180
M NTEKSAPTFHLGEVAHLQAEVQTGSHLPLQ H . . . LS.........Y.............L
210 210
M LFVDHCVATPSPLPDPNSSPYHFIVDFHG& H . . ..R.......--.QTA....V.......
240 238
M LVDGLSESFSAFQVPRPRPETLQFTVDVFH 270 H . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 M FA@SSRNTLYITCHLKVAPANQIPDKLNKA H . , . . . . . . I........T..,.T..E....
300 298
M CSFNKTSQSWLPVEGDADICDCCSHGNCSN H . . ..RS.K..S......EV.G...S.D.GS
330 328
M SSSSQFQIHGPRQWSKLVSRNRRHVTDEAD H ..R.RY.A..VS..P.SA..R....R....
360 358
M VTVGPLIFLGKANDQTVEGWTASAQTSVAL H . . . . . . . . . . ..S..A....AS.....L..
390 388
M GLGLATVAFLTLAAIVLAVTRKCHSSSSYLV H . . . ..A...........G...S..TP.HV.
420 418
M SLPQ 424 H ..S. 422 FIG. 4. Comparison of primary structures of mZP3 and hZP3 polypeptides. The single-letter amino acid code is used. Mouse (designated M) and hamster (designated H) polypeptides are aligned so as to provide maximum similarity between the polypeptides. The entire mZP3 amino acid sequence is shown. For hZP3, only those positions where the amino acid sequence differs from mZP3 are shown in the singleletter code. A two-amino acid deletion in hZP3 is indicated by dashes at positions 223 and 224 of mZP3. The predicted signal sequences are italicized (residues l-22), and locations of cysteine residues (C) and potential N-linked glycosylation sites (N) are underlined. [Here, hZP3 amino acid 303 is R.]
203
204
DEVELOPMENTALBIOLOGY
VOLUME 145,1991 5'-ATGCAGCGGGCCTTATTCAGGCAGTACC
ATG MET 1 TGT CYS 21 TCC SER 41 GGC GLY 61 CTG LEU 81
GGG CTG AGC GLY LEU SER
TAC CAG TYR GLN
TCC CAG SER GLN
CTG TGG CTC LEU TRP LEU
CCT PRO
CTC LEU
CTC LElJ
CTG TGT CTC CTG CTG TGT GGA LEU CYS LEIJ LEU LEV CYS GLY
TTG CCA LEU PRO
GGC GGA ACT GLY GLY THR
CCG ACC PRO THR
GTG GAG GTG GAG TGT CTG GAA VAL GLU VAL GLU cys LEU GLU
GCT GAG CTC GTG GTG ACT ALA GLU LEU VAL VAL THR
ACC THR
GAG GAC GLU ASP
GGG AAG GLY LYS
GTT TCC VAL SER
CTC ATA LEU ILE
GTG GCT ACG VAL ALA THR
CAG GLN
CCC PRO
CTC ACC CTT LEU THR LEU
GAT GTG GTC AGG TTC AAG ASP VAL VAL ARG PHE LYS
GCC ALA
CCA PRO
GGC GCC GLY ALA
AAG LYS
GGA AAG GLY LYS
CTC LEU
GTC AGT VAL SER
AGA ARG
GAC Asp
GGC TCA GLY SER
GAA GLU
AAC ASN
TGT CYS
CAG GLN
CAT HIS
GAA GLU
TGC CYS
TTG LEU
CAG GLN
TGC CYS 20 ACG TCA THR SER 40 CTT TTT LEu PHE 60 CGG CCC ARG PRO 80 AGC Aii SER ASN 100
AGG GTG CAG GTG ACG GAA GAT GCC CTG GTG TAC AGC ACC GTG CTG CTG CAC CAA CCC CGC
ARG 101 CCT PRO 121 TAC TYR 141 ACC THR 161 AAC ASN 181
VAL
GLN
GTG CCC VAL PRO
VAL
THR GLU
ASP
ALA
VAL
TYR
SER
GGC CTG TCC GLY LEU SER
ATC ILE
CTG AGG ACT LEU ARG THR
AAC ASN
CGT GCG GAC ARG ALA ASP
CCC PRO
AGG CAG GGC AAT ARG GLN GLY ASN
ACT THR
GTG TCC VAL SER
TCA SER
ACT THR
GAG AAA GLU LYS
TTG TCA LEU SER
GTG AGC VBL SEB
GAG GAG AAG GLU GLU LYS CCC PRO
ACC THR
LEU
AGC SER
CAC HIS
GCT ATC ALA ILE
CTG GTT TTC LEU VAL PHE TCC SER
CAC HIS
TCT SER
THR
VAL
LEU
LEU
GTG VAL
CCT PRO
CGG CCC ARG PRO
ACC THR
TGG TRP
CTC LEU
CTG ATG LEU MET
CGC ARG
CTG GGA GAG GTA LEU GLY GLU VAL
GCC ALA
TAC TYR
HIS
GLN
PRO
ARG 120 ATT GAG TGC CGC ILE GLU CYS ARG 140 GTT CCC TTC AGC VAL PRO PHE SER 160 GAG GAG AAC TGG GLU GLU ASN TRP 180 CTC CAG GCA GAG LEU GLN ALA GLU 7nn
GTC CAG ACT GGA AGC CAT CTG CCA CTG CTG CTG TTT GTG GAC CGC TGT GTG CCC ACA :CT
VAL 201 TCG SER 221 GAT ASP 241 CAG GLN 261 TGT CYS 281 TTC PHE 301 TGC CYS 321 CAG GLN 341 GTA VAL 361 TCT SER 381 GcT ALA 401 TCA SER 421
GLN
THR
GLY
SER
HIS
LEU
PRO
LEU
LEU
LEU
CCG PRO
GAC ASP
CAG GLN
ACC THR
GCC ALA
TCT SER
CCC PRO
TAT TYR
CAT HIS
GTC ATT VAL ILE
GGT CTA GLY LEU
TCT SER
GAG AGC GLU SER
TTT TCT PHE SER
GCA ALA
TTT CAA PHE GLN
TTC PHE
GTG GAT GTA VAL ASP VAL
TTC PHE
CAT HIS
TTT GCC PHE ALA
ACG THR
VAL
ASP
ARG
CYS
VAL
ALA
GTG GAC VAL ASP
TTC PHE
CAT HIS
GGT GLY
TGC CTT CYS LEU
GTG CCT AGA VAL PRO ARG
CCC PRO
CGG CCG ARG PRO
GAG ACT GLU THR
AAT BSN
AGC
TCC
AAT ASN
ACG THR
ATC ILE
TAT TYR
ACC
CCA PRO
GAT GAG CTC ASP GLU LEU
AAC ASN
AAA LYS
AGA ARG
CAT CTC AAA HIS LEU LYS
GTC ACC VAL THR
CCA PRO
GCC AAC ALA BSN
AAC &SN
AGG TCT ARG SEB
TCC SER
AGT SER
TGG TCG CCA GTA TRP SER PRO VAL
AGC SER
AGT SER
GGC GLY
GAC TGT GGT ASP CYS GLY
AGC SER
TCA SER
TGG CCC TRP PRO
AAG LYS
TCG GCA SER ALA
TCT SER
AGA ARG
CGC CGC AGG CAC ARG ARG ARG HIS
GGA CCC GLY PRO
CTG ATC LEU ILE
TTC PHE
CTG LEU
GGA AAG GCA GLY LYS ALA
AGC SER
GCT CAA ALA GLN
ACC THR
TTG GCT LEU ALA
CTT GGT TTA LEU GLY LEU
GGC CTA GCC GCA GTG GCA GLY LEU ALA ALA VAL ALA
TTC PHE
GCT ATT ALA ILE
GTC CTC VAL LEU
TGT CAC CYS HIS
GTT VAL
TCT SER
AAC LYS
CAG
PHE
AGC SER
GGT GTC ACC AGG AGT GLY VAL THR ARG SER
GAG GGC GAT GLU GLY ASP
GCT ALA
GAG GTC GLU VAL
CGT TCA CGG TAC ARG SER ARG TYR
CAG GLN
GCC ALA
CAT HIS
GTG AGA GAC VAL ARG ASP
GAA GLU
GCT ALA
GAC ASP
CAG GLN
ACC THR
GCT GTG GAG GGC ALA VAL GLU GLY
CCT PRO
TCC SER
CAT HIS
THR
ATC ILE
PRO 220 GTG VAL 240 CTT LEU 260 ACC THR
280 TGC TCC CYS SER 300 TGC GGC TGC CYS GLY Cys 320 GGA GTG AGC GLY VAL SER 340 GAT GTC ACG ASP VAL THR 360 TGG GCC TCT TRP ALA SER 380 CTG ACC CTG LEU THR LE" 400 GTA TCC CTT VAL SER LEIJ 420 GCC ALA
CAATAAAAAGTCCAGTTCTG-3' GLN
FIG. 3. Primary structure of hZP3 mRNA (top) locations of potential N-linked glycosylation respectively, and the codons for the following (AGC), and Asp-214 (GAC).]
and polypeptide (bottom). The predicted signal sequence (residues l-22) is italicized and sites (Asn-X-Ser/Thr) are underlined. [Here, hZP3 amino acids 251 and 303 are Gln and Arg, five amino acids have been corrected: Thr-54 (ACT), Glu-138 (GAG), Phe-159 (TTC), Ser-160
