Appl Microbiol Biotechnol DOI 10.1007/s00253-013-5423-7
APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY
Genomic structural variations contribute to trait improvement during whole-genome shuffling of yeast Dao-Qiong Zheng & Jie Chen & Ke Zhang & Ke-Hui Gao & Ou Li & Pin-Mei Wang & Xiao-Yang Zhang & Feng-Guang Du & Pei-Yong Sun & Ai-Min Qu & Shuang Wu & Xue-Chang Wu
Received: 31 July 2013 / Revised: 17 November 2013 / Accepted: 18 November 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Whole-genome shuffling (WGS) is a powerful technology of improving the complex traits of many microorganisms. However, the molecular mechanisms underlying the altered phenotypes in isolates were less clarified. Isolates with significantly enhanced stress tolerance and ethanol titer under very-high-gravity conditions were obtained after WGS of the bioethanol Saccharomyces cerevisiae strain ZTW1. Karyotype analysis and RT-qPCR showed that chromosomal rearrangement occurred frequently in genome shuffling. Thus, the phenotypic effects of genomic structural variations were determined in this study. RNA-Seq and physiological analyses revealed the diverse transcription pattern and physiological status of the isolate S3-110 and ZTW1. Our observations suggest that the improved stress tolerance of S3-110 can be largely attributed to the copy number variations in large DNA regions, which would adjust the ploidy of yeast cells and expression levels of certain genes involved in stress response. Overall, this work not only constructed shuffled S. cerevisiae strains that have potential industrial applications but also provided novel insights into the molecular mechanisms of WGS and enhanced our knowledge on this useful breeding strategy.
Dao-Qiong Zheng and Jie Chen contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00253-013-5423-7) contains supplementary material, which is available to authorized users. D.
APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY
Genomic structural variations contribute to trait improvement during whole-genome shuffling of yeast Dao-Qiong Zheng & Jie Chen & Ke Zhang & Ke-Hui Gao & Ou Li & Pin-Mei Wang & Xiao-Yang Zhang & Feng-Guang Du & Pei-Yong Sun & Ai-Min Qu & Shuang Wu & Xue-Chang Wu
Received: 31 July 2013 / Revised: 17 November 2013 / Accepted: 18 November 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Whole-genome shuffling (WGS) is a powerful technology of improving the complex traits of many microorganisms. However, the molecular mechanisms underlying the altered phenotypes in isolates were less clarified. Isolates with significantly enhanced stress tolerance and ethanol titer under very-high-gravity conditions were obtained after WGS of the bioethanol Saccharomyces cerevisiae strain ZTW1. Karyotype analysis and RT-qPCR showed that chromosomal rearrangement occurred frequently in genome shuffling. Thus, the phenotypic effects of genomic structural variations were determined in this study. RNA-Seq and physiological analyses revealed the diverse transcription pattern and physiological status of the isolate S3-110 and ZTW1. Our observations suggest that the improved stress tolerance of S3-110 can be largely attributed to the copy number variations in large DNA regions, which would adjust the ploidy of yeast cells and expression levels of certain genes involved in stress response. Overall, this work not only constructed shuffled S. cerevisiae strains that have potential industrial applications but also provided novel insights into the molecular mechanisms of WGS and enhanced our knowledge on this useful breeding strategy.
Dao-Qiong Zheng and Jie Chen contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00253-013-5423-7) contains supplementary material, which is available to authorized users. D.
