Institut für
Physiologie, Technische Universität München, Freising-Weihenstephan, Germany
805
HETEROLOGOUS RADIOIMMUNOASSAY FOR BOVINE FOLLICLE-STIMULATING HORMONE AND ITS APPLICATION DURING THE OESTROUS CYCLE IN CATTLE
By D. Schams and E.
Schallenberger
ABSTRACT A specific heterologous radioimmunoassay for bovine follicle-stimulating hormone (bFSH) has been developed using guinea pig antiserum to ovine FSH with radio-iodinated ovine FSH. Slight cross-reactions with bovine TSH and LH could be eliminated by adding bovine TSH and LH to each tube during the assay. The labelling method with lactoperoxidase turned out to be more useful than the chloramine-T method. Inhibition curves obtained with male and female bovine plasma were parallel to those obtained with the bovine standard preparation. Exogenous bFSH could be quantitatively recovered from 200 \g=m\lof plasma or serum with a mean of 101.7 \m=+-\7.7 %. The intra-assay coefficient of variation (CV) was xx 6 %. The inter-assay CV was 13.5 %. Sensitivity was 70 ng/ml NIH-FSH-B1. Mean plasma concentrations of FSH (NIH-FSH-B1) during the oestrous cycle were 303 \m=+-\101 ng/ml. When plasma samples were collected in 6 h intervals from 17 oestrous cycles from 13 regularly cycling heifers and cows, a significant FSH peak occurred just before and during oestrus and coincided with the preovulatory LH peak. Further FSH elevations could be measured on day 4, 8 and 12 after ovulation and 5 and 3 days before ovulation and showed a rhythmic pattern of four day intervals approximately.
The bovine species has to be considered as a species with a low FSH content in the pituitary (Rakha 8c Robertson 1965). Furthermore it is very difficult to obtain bovine FSH in a highly purified preparation and with a reasonable biological activity (Reichert 8c Jiang 1965). For this reason specific radio-
c
1.2
CO
£
5 >
1.0
0.8
(—
g
0.6
LU
°
Physiologie, Technische Universität München, Freising-Weihenstephan, Germany
805
HETEROLOGOUS RADIOIMMUNOASSAY FOR BOVINE FOLLICLE-STIMULATING HORMONE AND ITS APPLICATION DURING THE OESTROUS CYCLE IN CATTLE
By D. Schams and E.
Schallenberger
ABSTRACT A specific heterologous radioimmunoassay for bovine follicle-stimulating hormone (bFSH) has been developed using guinea pig antiserum to ovine FSH with radio-iodinated ovine FSH. Slight cross-reactions with bovine TSH and LH could be eliminated by adding bovine TSH and LH to each tube during the assay. The labelling method with lactoperoxidase turned out to be more useful than the chloramine-T method. Inhibition curves obtained with male and female bovine plasma were parallel to those obtained with the bovine standard preparation. Exogenous bFSH could be quantitatively recovered from 200 \g=m\lof plasma or serum with a mean of 101.7 \m=+-\7.7 %. The intra-assay coefficient of variation (CV) was xx 6 %. The inter-assay CV was 13.5 %. Sensitivity was 70 ng/ml NIH-FSH-B1. Mean plasma concentrations of FSH (NIH-FSH-B1) during the oestrous cycle were 303 \m=+-\101 ng/ml. When plasma samples were collected in 6 h intervals from 17 oestrous cycles from 13 regularly cycling heifers and cows, a significant FSH peak occurred just before and during oestrus and coincided with the preovulatory LH peak. Further FSH elevations could be measured on day 4, 8 and 12 after ovulation and 5 and 3 days before ovulation and showed a rhythmic pattern of four day intervals approximately.
The bovine species has to be considered as a species with a low FSH content in the pituitary (Rakha 8c Robertson 1965). Furthermore it is very difficult to obtain bovine FSH in a highly purified preparation and with a reasonable biological activity (Reichert 8c Jiang 1965). For this reason specific radio-
c
1.2
CO
£
5 >
1.0
0.8
(—
g
0.6
LU
°
