Proc. Nati. Acad. Sci. USA Vol. 89, pp. 286-289, January 1992 Chemistry
High-resolution tandem mass spectrometry of large biomolecules (electrospray ionization/polypeptide sequencing)
JOSEPH A. Loo*, JOHN P. QUINN, STEVEN I. Ryu, KENT D. HENRY, MICHAEL W. SENKO, AND FRED W. MCLAFFERTYt Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301
Contributed by Fred W. McLafferty, September 30, 1991
Unit-resolution mass spectra have been obABSTRACT tained for peptides as large as 17 kDa, providing information on impurities and adduct ions, as well as accurate molecular weight values. Electrospray ionization produces many multiply-charged species of the same mass; isotopic peak resolution provides direct charge state assignment from the unit mass spacing of the isotopes. This is of special value when the spectrum also has many masses, such as from precursor ion dissociation or impurities. Mass measuring errors not only are concomitantly lower (
High-resolution tandem mass spectrometry of large biomolecules (electrospray ionization/polypeptide sequencing)
JOSEPH A. Loo*, JOHN P. QUINN, STEVEN I. Ryu, KENT D. HENRY, MICHAEL W. SENKO, AND FRED W. MCLAFFERTYt Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301
Contributed by Fred W. McLafferty, September 30, 1991
Unit-resolution mass spectra have been obABSTRACT tained for peptides as large as 17 kDa, providing information on impurities and adduct ions, as well as accurate molecular weight values. Electrospray ionization produces many multiply-charged species of the same mass; isotopic peak resolution provides direct charge state assignment from the unit mass spacing of the isotopes. This is of special value when the spectrum also has many masses, such as from precursor ion dissociation or impurities. Mass measuring errors not only are concomitantly lower (
