Article pubs.acs.org/ac
Highly Flexible UV−Vis Radiation Sources and Novel Detection Schemes for Spectrophotometric HPLC Detection Karsten G. Kraiczek,*,†,§ R. Bonjour,‡ Y. Salvadé,‡ and Roland Zengerle§ †
Agilent Technologies, Hewlett-Packard Str. 8, D 76337 Waldbronn, Germany Haute Ecole Arc Ingénierie, University of Applied Sciences, 2610 St.Imier, Switzerland § IMTEK − Department of Microsystems Engineering, University of Freiburg, D-79110 Freiburg, Germany ‡
S Supporting Information *
ABSTRACT: The concept and performance of the first multiwavelength deep UV light-emitting-diode-based high-performance liquid chromatography (HPLC) absorbance detector are presented. In single-wavelength mode and with optical reference, the limit of detection (LOD) is comparable to conventional state-of-theart HPLC absorbance detectors. In multiwavelength modeat present up to eight wavelengths without optical referencethe LOD is about 10 times higher than in single-wavelength mode. Multiplexing and demultiplexing methods are used to separate chromatographic signals in multiwavelength mode and keeps the detector configuration simple and yet flexible. Depending on the operation mode, stray light is either totally negligible or controlled electronically and digitally.
H
for what is required for spectrophotometric HPLC detection (
Highly Flexible UV−Vis Radiation Sources and Novel Detection Schemes for Spectrophotometric HPLC Detection Karsten G. Kraiczek,*,†,§ R. Bonjour,‡ Y. Salvadé,‡ and Roland Zengerle§ †
Agilent Technologies, Hewlett-Packard Str. 8, D 76337 Waldbronn, Germany Haute Ecole Arc Ingénierie, University of Applied Sciences, 2610 St.Imier, Switzerland § IMTEK − Department of Microsystems Engineering, University of Freiburg, D-79110 Freiburg, Germany ‡
S Supporting Information *
ABSTRACT: The concept and performance of the first multiwavelength deep UV light-emitting-diode-based high-performance liquid chromatography (HPLC) absorbance detector are presented. In single-wavelength mode and with optical reference, the limit of detection (LOD) is comparable to conventional state-of-theart HPLC absorbance detectors. In multiwavelength modeat present up to eight wavelengths without optical referencethe LOD is about 10 times higher than in single-wavelength mode. Multiplexing and demultiplexing methods are used to separate chromatographic signals in multiwavelength mode and keeps the detector configuration simple and yet flexible. Depending on the operation mode, stray light is either totally negligible or controlled electronically and digitally.
H
for what is required for spectrophotometric HPLC detection (
