LAB/IN VITRO RESEARCH e-ISSN 1643-3750 © Med Sci Monit, 2015; 21: 1856-1863 DOI: 10.12659/MSM.893528
HOTAIR Interacting with MAPK1 Regulates Ovarian Cancer skov3 Cell Proliferation, Migration, and Invasion
Received: 2015.01.09 Accepted: 2015.02.16 Published: 2015.06.28
Authors’ Contribution: Study Design A Data Collection B Statistical Analysis C Data Interpretation D Manuscript Preparation E Literature Search F Funds Collection G
ABCDEF 1,2,3 B 4 F 1,2 DE 1,2 DEFG 1,2,3
Tang Yiwei Huang Hua Guo Hui Meng Mao Long Xiang
1 Laboratory of Early Developmental and Injuries, West China Institutes for Woman and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, P.R. China 2 Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu, Sichuan, P.R. China 3 Department of Pediatrics, Chengdu first people’s Hospital, Chengdu Integrated TCM&Western Medicine Hospital, Chengdu, Sichuan, P.R. China 4 Department of Obstetrics and Gynecology, Chengdu First People’s Hospital, Chengdu Integrated TCM&Western Medicine Hospital, Chengdu, Sichuan, P.R. China
Corresponding Author: Source of support:
Background:
Material/Methods:
Results:
Conclusions:
MeSH Keywords:
MicroRNAs • Ovarian Neoplasms • RNA, Long Noncoding
Full-text PDF:
http://www.medscimonit.com/abstract/index/idArt/893528
Long Xiang, e-mail: [email protected] Departmental sources
The aim of this study was to evaluate the effect of when silencing HOTAIR in ovarian cancer skov3 cells on proliferation, migration, and invasion, and to elucidate the mechanism by which this occurs. We detected the mRNA level of HOTAIR (HOX antisense intergenic RNA) and MAPK1 (mitogen-activated protein kinase 1) in ovarian cancer SKOV3, ES-2, OVCAR3, A2780, and COC1 cell lines. We detected the mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 when transected with miR-1, miR-214-3p, or miR-330-5p. We detected the mRNA and protein level of MAPK1 when silencing HOTAIR. We detected the expression of HOTAIR when silencing MAPK1. Then we detected the proliferation, migration, and invasion in ovarian cancer skov3 after silencing HOTAIR or MAPK1. The expression of HOTAIR and MAPK1 in ovarian SKOV3, ES-2, and OVCAR3 increased compared with A2780 and COC1 cells (P
HOTAIR Interacting with MAPK1 Regulates Ovarian Cancer skov3 Cell Proliferation, Migration, and Invasion
Received: 2015.01.09 Accepted: 2015.02.16 Published: 2015.06.28
Authors’ Contribution: Study Design A Data Collection B Statistical Analysis C Data Interpretation D Manuscript Preparation E Literature Search F Funds Collection G
ABCDEF 1,2,3 B 4 F 1,2 DE 1,2 DEFG 1,2,3
Tang Yiwei Huang Hua Guo Hui Meng Mao Long Xiang
1 Laboratory of Early Developmental and Injuries, West China Institutes for Woman and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, P.R. China 2 Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu, Sichuan, P.R. China 3 Department of Pediatrics, Chengdu first people’s Hospital, Chengdu Integrated TCM&Western Medicine Hospital, Chengdu, Sichuan, P.R. China 4 Department of Obstetrics and Gynecology, Chengdu First People’s Hospital, Chengdu Integrated TCM&Western Medicine Hospital, Chengdu, Sichuan, P.R. China
Corresponding Author: Source of support:
Background:
Material/Methods:
Results:
Conclusions:
MeSH Keywords:
MicroRNAs • Ovarian Neoplasms • RNA, Long Noncoding
Full-text PDF:
http://www.medscimonit.com/abstract/index/idArt/893528
Long Xiang, e-mail: [email protected] Departmental sources
The aim of this study was to evaluate the effect of when silencing HOTAIR in ovarian cancer skov3 cells on proliferation, migration, and invasion, and to elucidate the mechanism by which this occurs. We detected the mRNA level of HOTAIR (HOX antisense intergenic RNA) and MAPK1 (mitogen-activated protein kinase 1) in ovarian cancer SKOV3, ES-2, OVCAR3, A2780, and COC1 cell lines. We detected the mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 when transected with miR-1, miR-214-3p, or miR-330-5p. We detected the mRNA and protein level of MAPK1 when silencing HOTAIR. We detected the expression of HOTAIR when silencing MAPK1. Then we detected the proliferation, migration, and invasion in ovarian cancer skov3 after silencing HOTAIR or MAPK1. The expression of HOTAIR and MAPK1 in ovarian SKOV3, ES-2, and OVCAR3 increased compared with A2780 and COC1 cells (P
