Nucleic Acids Research, Vol. 18, No. 7 1901
.fj 1990 Oxford University Press
Human enteric adenovirus type 41 (Tak) contains a second fiber protein gene Norman J.Pieniazek1, Susan B.Slemendal, Danuta Pieniazek2, Jorge Velarde Jr and Ronald B.Luftig* Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112-1393, 'Parasitic Diseases Branch and 2AIDS Program, Centers for Disease Control, Atlanta, GA 30333, USA EMBL accession no. X17016
Submitted February 23, 1990
position 83.1 % to the AccI site at map position 87.1 %. Bases 1161 to 1402 overlap the sequence published earlier by us (2).
DNA sequencing of the human enteric adenovirus type 41 Tak (1) genome upstream from the fiber protein gene (2) revealed an open reading frame of 387 amino acids coding for a 41.4K protein. The first 42 amino acids of this protein show a high degree of homology both to Ad41 (74%) and Ad2 (61 %) 60K fiber protein tail domains (2, 3). Further, amino acids 43 to 233 form a typical shaft domain of twelve 15-residue repetitive motifs which is in contrast to 22 such repeats found for Ad2 (3), AdS (4), and Ad41 or 6 repeats found for Ad3 (5) and Ad7 (6). The knob domain (amino acids 234 to 387) is about 15% shorter than found for above mentioned viruses. If this gene is expressed, Ad41 would resemble avian adenoviruses which were found to have two fibers of different size protruding from their pentons (7). The sequence presented below is from the EcoRV site at map
REFERENCES 1. van Loon,A.E., Rozijn,T.H., de Jong,J.C. and Sussenbach,J.S. (1985) Virology 140, 197-200. 2. Pieniazek,N.J., Slemenda,S.B., Pieniazek,D., Velarde,J.,Jr. and Luftig,R.B. (1989) Nucl. Acids Res. 17, 9474. 3. Herisse,J., Rigolet,M., Dupont de Dinechin,S. and Galibert,F. (1981) Nucl. Acids Res. 9, 4023-4042. 4. Chroboczek,J. and Jacrot,B. (1987) Virology 161, 549-554. 5. Signas,C., Akusjarvi,G. and Pettersson,U. (1985) J. Virol. 53, 672-678. 6. Hong,J.S., Mullis,K.G. and Engler,J.A. (1988) Virology 167, 545-553. 7. Laver,W.G., Younghusband,H.B. and Wrigley,N.G. (1971) Virology 45,
598-614. 120
EcoRV
41.4K fiber protein: N K R T R I K D D F N P V Y P Y D T F S T P S I P Y V A kCCTTTTTACCCTGACCCACGATCTTCkTCTTGCAGkTGAAkAGAACCAGkATTGAAGACGACTTCAACCCCGTCTACCCCTATGASACCTTCTCkACTCCCAGCkTCCCCTkTGTAGCT AccI splice acceptor
240
P P F V S S D G L Q E K P P G V L A L K Y T D P I T T N A K H E L T L K L G S N CCGCCCTTCGTTTCTTCTGACGGGTTCAGAkACCCCAGGAGTTTTAGCACTCAAGTACACTGACCCCkTTACTACCAkTGCTAAGCkTGAGCTTACTTTAAAACTTGACC
360
I T L E N G L L S A T V P T V S P P L T N S N N S L G L k T S A P I k V S A N S ATAkCTTTAGAAAkTGGGTTACTTTCGGCCACAGTTCCCACTGTTTCTCCTCCCCTTACAAACAGTAACAACTCCCTGGGTTTAGCCACATCCGCTCCCATAGCTGTATCAGCTAACTCT
480
L T L A T AkA P L T V S N N Q L S I N A G R G L V I T N N A L T V N P T G A L G CTCACATTGGCCACCGCCGCACCACTGACAGTAAGCAACkACCAGCTTAGTATTAACGCGGGCAGAGGTTTAGTTATAACTAACkATGCCTTAACAGTTAkTCCTACCGGAGCGCTAGGT
600
N N T G k L Q L N A k G G M R V D G A N L I L B V A Y P F E A I N Q L T L R L TTCAATAACACAGGAGCTTTACAATTAkATGCTGCAGGAGGAATGAGAGTGGACGGTGCCkACTTAATTCTTCATGTAGCATATCCCST GAAGCAkTCkACCAGCTAACACTGCGkTTA
720
N G L E V T S G G X L N V X L G S G L Q F D S N G R I A I S N S N R T R S V P GAAkACGGGTTAGAAGTAACCAGCGGAGGAAAGCTTAACGTTAAGTTGGGQTCAGGCCTCCAATTTGACAGTAACGGACGCATTGCTATTAGTAATAGCAACCGAACTCGAAGTGTACCk
840
S L T T I W S I S P T P N C S I Y E T Q D k N L F L C L T K N G A H V L G T I T TCCCTCACTACCATTTGGTCTATCTCGCCTACGCCTAACTGCTCCkTTTATGAAACCCAAGATGCAAACCTkTTTCTTTGTCTAACTAAAAACGGAGCTCACGTATTAGGTACTATAACA
960
I K G L K G A L R 9 M H D N k L S L K L P F D N Q G N L L N C A L E 8 S T W R Y ATCAkAGGTCTTAAAGGAGCACTGCGGGAAATGCACGkTAACGCTCTATCTTTAAAACTTCCCTTTGACAkTChGGAAkTTTACTTAACTGTGCCTTGGAATCATCCACCTGGCGTTAC
1080
F
E
Q E
T
N
A
V
A
S
N
A
L
T
F
N
P
N
S
T
V
Y
P
R
N
K
T
A
H
P
G
N
M
L
I
Q
I
S
P
N
I
T
CAGGAkACCAACGCAGTGGCCTCTAATGCCTTAACATTTATGCCCAACAGTACAGTGTATCCACGAAACAAAACCGCTCACCCGGGCAACATGCTCkTCCAAATCTCGCCTAACkTCACC F
S
V
V
Y
N
E
I
N
S
G
Y
A
F
T
F
K
W
S
A
E
P
G
K
P
F
Smal B P P
T
A
V
F
C
Y
I
T
E
1200
Q
TTCAGTGTCGTCTACAACGAGATAAACAGTGGGTATGCTTTTACTTTTAAkTGGTCAGCCGAACCGGGAAAACCTTTTCACCCACCTACCGCTGTATTTTGCTACkTAACTGACATAA 1320 polyA signal AccI 60 .6K f iber protein ( protein IV): M K R A R L E D D F N P V Y. . AATCATTGCAGGCACAATCTTCGCATTTXTTTTTTTCCAGkTGAAkGAGCCAGAC TTGAAGkTGAC TTCAACCCCGTC TAC AccI splice acceptor *
To whom correspondence should be addressed
1 40 2
.fj 1990 Oxford University Press
Human enteric adenovirus type 41 (Tak) contains a second fiber protein gene Norman J.Pieniazek1, Susan B.Slemendal, Danuta Pieniazek2, Jorge Velarde Jr and Ronald B.Luftig* Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112-1393, 'Parasitic Diseases Branch and 2AIDS Program, Centers for Disease Control, Atlanta, GA 30333, USA EMBL accession no. X17016
Submitted February 23, 1990
position 83.1 % to the AccI site at map position 87.1 %. Bases 1161 to 1402 overlap the sequence published earlier by us (2).
DNA sequencing of the human enteric adenovirus type 41 Tak (1) genome upstream from the fiber protein gene (2) revealed an open reading frame of 387 amino acids coding for a 41.4K protein. The first 42 amino acids of this protein show a high degree of homology both to Ad41 (74%) and Ad2 (61 %) 60K fiber protein tail domains (2, 3). Further, amino acids 43 to 233 form a typical shaft domain of twelve 15-residue repetitive motifs which is in contrast to 22 such repeats found for Ad2 (3), AdS (4), and Ad41 or 6 repeats found for Ad3 (5) and Ad7 (6). The knob domain (amino acids 234 to 387) is about 15% shorter than found for above mentioned viruses. If this gene is expressed, Ad41 would resemble avian adenoviruses which were found to have two fibers of different size protruding from their pentons (7). The sequence presented below is from the EcoRV site at map
REFERENCES 1. van Loon,A.E., Rozijn,T.H., de Jong,J.C. and Sussenbach,J.S. (1985) Virology 140, 197-200. 2. Pieniazek,N.J., Slemenda,S.B., Pieniazek,D., Velarde,J.,Jr. and Luftig,R.B. (1989) Nucl. Acids Res. 17, 9474. 3. Herisse,J., Rigolet,M., Dupont de Dinechin,S. and Galibert,F. (1981) Nucl. Acids Res. 9, 4023-4042. 4. Chroboczek,J. and Jacrot,B. (1987) Virology 161, 549-554. 5. Signas,C., Akusjarvi,G. and Pettersson,U. (1985) J. Virol. 53, 672-678. 6. Hong,J.S., Mullis,K.G. and Engler,J.A. (1988) Virology 167, 545-553. 7. Laver,W.G., Younghusband,H.B. and Wrigley,N.G. (1971) Virology 45,
598-614. 120
EcoRV
41.4K fiber protein: N K R T R I K D D F N P V Y P Y D T F S T P S I P Y V A kCCTTTTTACCCTGACCCACGATCTTCkTCTTGCAGkTGAAkAGAACCAGkATTGAAGACGACTTCAACCCCGTCTACCCCTATGASACCTTCTCkACTCCCAGCkTCCCCTkTGTAGCT AccI splice acceptor
240
P P F V S S D G L Q E K P P G V L A L K Y T D P I T T N A K H E L T L K L G S N CCGCCCTTCGTTTCTTCTGACGGGTTCAGAkACCCCAGGAGTTTTAGCACTCAAGTACACTGACCCCkTTACTACCAkTGCTAAGCkTGAGCTTACTTTAAAACTTGACC
360
I T L E N G L L S A T V P T V S P P L T N S N N S L G L k T S A P I k V S A N S ATAkCTTTAGAAAkTGGGTTACTTTCGGCCACAGTTCCCACTGTTTCTCCTCCCCTTACAAACAGTAACAACTCCCTGGGTTTAGCCACATCCGCTCCCATAGCTGTATCAGCTAACTCT
480
L T L A T AkA P L T V S N N Q L S I N A G R G L V I T N N A L T V N P T G A L G CTCACATTGGCCACCGCCGCACCACTGACAGTAAGCAACkACCAGCTTAGTATTAACGCGGGCAGAGGTTTAGTTATAACTAACkATGCCTTAACAGTTAkTCCTACCGGAGCGCTAGGT
600
N N T G k L Q L N A k G G M R V D G A N L I L B V A Y P F E A I N Q L T L R L TTCAATAACACAGGAGCTTTACAATTAkATGCTGCAGGAGGAATGAGAGTGGACGGTGCCkACTTAATTCTTCATGTAGCATATCCCST GAAGCAkTCkACCAGCTAACACTGCGkTTA
720
N G L E V T S G G X L N V X L G S G L Q F D S N G R I A I S N S N R T R S V P GAAkACGGGTTAGAAGTAACCAGCGGAGGAAAGCTTAACGTTAAGTTGGGQTCAGGCCTCCAATTTGACAGTAACGGACGCATTGCTATTAGTAATAGCAACCGAACTCGAAGTGTACCk
840
S L T T I W S I S P T P N C S I Y E T Q D k N L F L C L T K N G A H V L G T I T TCCCTCACTACCATTTGGTCTATCTCGCCTACGCCTAACTGCTCCkTTTATGAAACCCAAGATGCAAACCTkTTTCTTTGTCTAACTAAAAACGGAGCTCACGTATTAGGTACTATAACA
960
I K G L K G A L R 9 M H D N k L S L K L P F D N Q G N L L N C A L E 8 S T W R Y ATCAkAGGTCTTAAAGGAGCACTGCGGGAAATGCACGkTAACGCTCTATCTTTAAAACTTCCCTTTGACAkTChGGAAkTTTACTTAACTGTGCCTTGGAATCATCCACCTGGCGTTAC
1080
F
E
Q E
T
N
A
V
A
S
N
A
L
T
F
N
P
N
S
T
V
Y
P
R
N
K
T
A
H
P
G
N
M
L
I
Q
I
S
P
N
I
T
CAGGAkACCAACGCAGTGGCCTCTAATGCCTTAACATTTATGCCCAACAGTACAGTGTATCCACGAAACAAAACCGCTCACCCGGGCAACATGCTCkTCCAAATCTCGCCTAACkTCACC F
S
V
V
Y
N
E
I
N
S
G
Y
A
F
T
F
K
W
S
A
E
P
G
K
P
F
Smal B P P
T
A
V
F
C
Y
I
T
E
1200
Q
TTCAGTGTCGTCTACAACGAGATAAACAGTGGGTATGCTTTTACTTTTAAkTGGTCAGCCGAACCGGGAAAACCTTTTCACCCACCTACCGCTGTATTTTGCTACkTAACTGACATAA 1320 polyA signal AccI 60 .6K f iber protein ( protein IV): M K R A R L E D D F N P V Y. . AATCATTGCAGGCACAATCTTCGCATTTXTTTTTTTCCAGkTGAAkGAGCCAGAC TTGAAGkTGAC TTCAACCCCGTC TAC AccI splice acceptor *
To whom correspondence should be addressed
1 40 2
