363
Biochimica et Biophysica Acta, 536 (1978) 363--375 © Elsevier/North-Holland Biomedical Press
BBA 38009
HUMAN P I T U I T A R Y T H Y R O T R O P I N ISOLATION OF ~ AND/3 SUBUNITS BY H Y D R O P H O B I C INTERACTION CHROMATOGRAPHY
GUNILLA JACOBSON, PAUL ROOS and LEIF WIDE
Institute of Biochemistry, Biomedical Center, University of Uppsala, and Department of Clinical Chemistry, University Hospital, Uppsala (Sweden) (Received March 28th, 1978)
Summary Various conditions for the dissociation of highly purified human pituitary thyrotropin into subunits have been investigated. Dissociation on a preparative scale was accomplished by treatment with 1 M propionic acid at 32°C for 16 h. The isolation of one ~ and two /3 subunits was achieved by hydrophobic interaction chromatography on pentyl-Sepharose-4B. Radioimmunological technique was utilized to classify the subunits in accordance with current nomenclature and also to express their activities. The activities of the subunits overlapped insignificantly (40.3%) and their content of intact thyrotropin was negligible (
Biochimica et Biophysica Acta, 536 (1978) 363--375 © Elsevier/North-Holland Biomedical Press
BBA 38009
HUMAN P I T U I T A R Y T H Y R O T R O P I N ISOLATION OF ~ AND/3 SUBUNITS BY H Y D R O P H O B I C INTERACTION CHROMATOGRAPHY
GUNILLA JACOBSON, PAUL ROOS and LEIF WIDE
Institute of Biochemistry, Biomedical Center, University of Uppsala, and Department of Clinical Chemistry, University Hospital, Uppsala (Sweden) (Received March 28th, 1978)
Summary Various conditions for the dissociation of highly purified human pituitary thyrotropin into subunits have been investigated. Dissociation on a preparative scale was accomplished by treatment with 1 M propionic acid at 32°C for 16 h. The isolation of one ~ and two /3 subunits was achieved by hydrophobic interaction chromatography on pentyl-Sepharose-4B. Radioimmunological technique was utilized to classify the subunits in accordance with current nomenclature and also to express their activities. The activities of the subunits overlapped insignificantly (40.3%) and their content of intact thyrotropin was negligible (
