AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 8, Number 8, 1992 Mary Ann Liebert, Inc., Publishers
Humoral Immune Responses to Homologous Envelope Peptides in Vaccinées and Lab Workers Infected with HIV SETH H. PINCUS and KATHERINE MESSER
WE resenting peptides
overlapping Geysen peptides repthe entire HXB-2 envelope sequence. The set of consisted of 283 12-mers that started every third amino acid (i.e., there was an overlap of 9 amino acids between adjacent peptides). The following samples were tested: ( 1 ) serial samples obtained from laboratory workers infected with the HTLV-1IIB strain of HIV, (2) vacinees receiving either recombinant gpl60 or a combination of vaccinia-gpl60 and rgpl60, (3) a pool of sera from HIV seropositive individuals, and (4) normal seronegatives. Both infected laboratory workers made antibodies to welldescribed epitopes on the tip of the V3 loop, the putative CD4-binding region, and the gp41 immunodominant region. Surprisingly, high levels of antibodies were also seen to a peptide within the V1 loop of gp 120 (SSSGRMIMEKGE) and to the C-terminal 25 AAs of gp41. Vaccinées produced lower levels of antibodies to gpl60 than the infected lab workers. Those vaccinées who produced ade-
Downloaded by Iowa State Univ from www.liebertpub.com at 01/12/19. For personal use only.
have constructed
NIAID
Rocky Mountain Laboratories. Hamilton,
quate quantities of antibody to measure on Geysen peptides had
a more restricted antipeptide response focused on the V3 loop and an epitope in mid-gp41 (PRGPDRPEGIEEEGGERD). The V3 loop antibodies in the vaccinées had maximal binding 6 AAs to the carboxy-side of the peak binding seen in infected lab workers. Thus there are differences in both the magnitude and the epitope specificity of the anti-gpl60 humoral immune response of vaccinated and immunized humans. We thank David Schwartz and George Lewis for vaccinée sera; William Blattner and Ginga Coleclough for the lab worker sera.
MT 59840.
1347
Address
Rocky
reprint requests
to:
Seth H. Pincus NIAID Mountain Laboratories Hamilton, MT 59840
Humoral Immune Responses to Homologous Envelope Peptides in Vaccinées and Lab Workers Infected with HIV SETH H. PINCUS and KATHERINE MESSER
WE resenting peptides
overlapping Geysen peptides repthe entire HXB-2 envelope sequence. The set of consisted of 283 12-mers that started every third amino acid (i.e., there was an overlap of 9 amino acids between adjacent peptides). The following samples were tested: ( 1 ) serial samples obtained from laboratory workers infected with the HTLV-1IIB strain of HIV, (2) vacinees receiving either recombinant gpl60 or a combination of vaccinia-gpl60 and rgpl60, (3) a pool of sera from HIV seropositive individuals, and (4) normal seronegatives. Both infected laboratory workers made antibodies to welldescribed epitopes on the tip of the V3 loop, the putative CD4-binding region, and the gp41 immunodominant region. Surprisingly, high levels of antibodies were also seen to a peptide within the V1 loop of gp 120 (SSSGRMIMEKGE) and to the C-terminal 25 AAs of gp41. Vaccinées produced lower levels of antibodies to gpl60 than the infected lab workers. Those vaccinées who produced ade-
Downloaded by Iowa State Univ from www.liebertpub.com at 01/12/19. For personal use only.
have constructed
NIAID
Rocky Mountain Laboratories. Hamilton,
quate quantities of antibody to measure on Geysen peptides had
a more restricted antipeptide response focused on the V3 loop and an epitope in mid-gp41 (PRGPDRPEGIEEEGGERD). The V3 loop antibodies in the vaccinées had maximal binding 6 AAs to the carboxy-side of the peak binding seen in infected lab workers. Thus there are differences in both the magnitude and the epitope specificity of the anti-gpl60 humoral immune response of vaccinated and immunized humans. We thank David Schwartz and George Lewis for vaccinée sera; William Blattner and Ginga Coleclough for the lab worker sera.
MT 59840.
1347
Address
Rocky
reprint requests
to:
Seth H. Pincus NIAID Mountain Laboratories Hamilton, MT 59840
