Hydrolysis enhances bioavailability of proanthocyanidin-derived metabolites and improves β-cell function in glucose intolerant rats Kaiyuan Yang, Zohre Hashemi, Wei Han, Alena Jin, Han Yang, Jocelyn Ozga, Liang Li, Catherine B. Chan PII: DOI: Reference:
S0955-2863(15)00075-3 doi: 10.1016/j.jnutbio.2015.03.002 JNB 7336
To appear in:
The Journal of Nutritional Biochemistry
Received date: Revised date: Accepted date:
8 September 2014 1 March 2015 10 March 2015
Please cite this article as: Yang Kaiyuan, Hashemi Zohre, Han Wei, Jin Alena, Yang Han, Ozga Jocelyn, Li Liang, Chan Catherine B., Hydrolysis enhances bioavailability of proanthocyanidin-derived metabolites and improves β-cell function in glucose intolerant rats, The Journal of Nutritional Biochemistry (2015), doi: 10.1016/j.jnutbio.2015.03.002
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Hydrolysis enhances bioavailability of proanthocyanidin-derived metabolites and
RI P
T
improves β-cell function in glucose intolerant rats
Kaiyuan Yang1, Zohre Hashemi1, Wei Han2, Alena Jin1, Han Yang1, Jocelyn Ozga1, Liang Li2,
1
NU
SC
Catherine B. Chan1, 3
Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB
MA
Canada
Department of Chemistry, University of Alberta, Edmonton, AB Canada
3
Department of Physiology, University of Alberta, Edmonton, AB Canada
PT
ED
2
Corresponding Author: Catherine B. Chan; 6-126B Li Ka Shing Centre for Health Innovation
CE
Research, University of Alberta, Edmonton, Alberta, Canada T6G 2R3; Tel: 1-780-492-9939; E-
AC
mail: [email protected]
Running Title: Bioavailability and effects of proanthocyanidins on β-cell function
Grants: Alberta Innovates BioSolutions and the Alberta Pulse Growers Commission
1
ACCEPTED MANUSCRIPT Abstract Proanthocyanidins (PAC) are a highly consumed class of flavonoids and their consumption has
T
been linked to beneficial effects in type 2 diabetes. However, limited gastrointestinal absorption
RI P
occurs due to the polymeric structure of PAC. We hypothesized that hydrolysis of the PAC polymer would increase bioavailability, thus leading to enhanced beneficial effects on glucose
SC
homeostasis and pancreatic β-cell function. PAC-rich pea seed coats (PSC) were supplemented
NU
to a high fat diet (HFD) either in native (PAC) or hydrolyzed (HPAC) form fed to rats for 4 weeks. HFD or low fat diet (LFD) groups were controls. PAC-derived compounds were characterized in
MA
both PSC and serum. Glucose- and insulin-tolerance tests were conducted. Pancreatic α- and βcell areas and glucose-stimulated insulin secretion (GSIS) from isolated islets were measured.
ED
Increased PAC-derived metabolites were detected in the serum of HPAC-fed rats compared to PAC-fed rats, suggesting hydrolysis of PSC enhanced PAC bioavailability. This was associated
PT
with ~18% less (P
S0955-2863(15)00075-3 doi: 10.1016/j.jnutbio.2015.03.002 JNB 7336
To appear in:
The Journal of Nutritional Biochemistry
Received date: Revised date: Accepted date:
8 September 2014 1 March 2015 10 March 2015
Please cite this article as: Yang Kaiyuan, Hashemi Zohre, Han Wei, Jin Alena, Yang Han, Ozga Jocelyn, Li Liang, Chan Catherine B., Hydrolysis enhances bioavailability of proanthocyanidin-derived metabolites and improves β-cell function in glucose intolerant rats, The Journal of Nutritional Biochemistry (2015), doi: 10.1016/j.jnutbio.2015.03.002
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Hydrolysis enhances bioavailability of proanthocyanidin-derived metabolites and
RI P
T
improves β-cell function in glucose intolerant rats
Kaiyuan Yang1, Zohre Hashemi1, Wei Han2, Alena Jin1, Han Yang1, Jocelyn Ozga1, Liang Li2,
1
NU
SC
Catherine B. Chan1, 3
Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB
MA
Canada
Department of Chemistry, University of Alberta, Edmonton, AB Canada
3
Department of Physiology, University of Alberta, Edmonton, AB Canada
PT
ED
2
Corresponding Author: Catherine B. Chan; 6-126B Li Ka Shing Centre for Health Innovation
CE
Research, University of Alberta, Edmonton, Alberta, Canada T6G 2R3; Tel: 1-780-492-9939; E-
AC
mail: [email protected]
Running Title: Bioavailability and effects of proanthocyanidins on β-cell function
Grants: Alberta Innovates BioSolutions and the Alberta Pulse Growers Commission
1
ACCEPTED MANUSCRIPT Abstract Proanthocyanidins (PAC) are a highly consumed class of flavonoids and their consumption has
T
been linked to beneficial effects in type 2 diabetes. However, limited gastrointestinal absorption
RI P
occurs due to the polymeric structure of PAC. We hypothesized that hydrolysis of the PAC polymer would increase bioavailability, thus leading to enhanced beneficial effects on glucose
SC
homeostasis and pancreatic β-cell function. PAC-rich pea seed coats (PSC) were supplemented
NU
to a high fat diet (HFD) either in native (PAC) or hydrolyzed (HPAC) form fed to rats for 4 weeks. HFD or low fat diet (LFD) groups were controls. PAC-derived compounds were characterized in
MA
both PSC and serum. Glucose- and insulin-tolerance tests were conducted. Pancreatic α- and βcell areas and glucose-stimulated insulin secretion (GSIS) from isolated islets were measured.
ED
Increased PAC-derived metabolites were detected in the serum of HPAC-fed rats compared to PAC-fed rats, suggesting hydrolysis of PSC enhanced PAC bioavailability. This was associated
PT
with ~18% less (P
