Journal of Veterinary Diagnostic Investigation http://vdi.sagepub.com/
Improved diagnostic performance of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 −glutathione S-transferase fusion protein as antigen Chungwon Chung, Carey Wilson, Chandima-Bandara Bandaranayaka-Mudiyanselage, Eunah Kang, D. Scott Adams, Lowell S. Kappmeyer, Donald P. Knowles, Terry F. McElwain, James F. Evermann, Massaro W. Ueti, Glen A. Scoles, Stephen S. Lee and Travis C. McGuire J VET Diagn Invest published online 6 December 2013 DOI: 10.1177/1040638713511813 The online version of this article can be found at: http://vdi.sagepub.com/content/early/2013/12/06/1040638713511813
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Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
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Downloaded from vdi.sagepub.com at Scientific library of Moscow State University on December 26, 2013
511813 research-article2013
VDIXXX10.1177/1040638713511813Chung et al.Improved performance of Anaplasma antibody cELISA
Article
Improved diagnostic performance of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5–glutathione S-transferase fusion protein as antigen
Journal of Veterinary Diagnostic Investigation XX(X) 1–11 © 2013 The Author(s) Reprints and permissions: sagepub.com/journalsPermissions.nav DOI: 10.1177/1040638713511813 jvdi.sagepub.com
Chungwon Chung,1 Carey Wilson, Chandima-Bandara Bandaranayaka-Mudiyanselage, Eunah Kang, D. Scott Adams, Lowell S. Kappmeyer, Donald P. Knowles, Terry F. McElwain, James F. Evermann, Massaro W. Ueti, Glen A. Scoles, Stephen S. Lee, Travis C. McGuire
Abstract. The current study tested the hypothesis that removal of maltose binding protein (MBP) from recombinant antigen used for plate coating would improve the specificity of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay (cELISA). The number of 358 sera with significant MBP antibody binding (≥30%I) in Anaplasmanegative herds was 139 (38.8%) when tested using the recombinant major surface protein 5 (rMSP5)-MBP cELISA without MBP adsorption. All but 8 of the MBP binders were rendered negative (
Improved diagnostic performance of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 −glutathione S-transferase fusion protein as antigen Chungwon Chung, Carey Wilson, Chandima-Bandara Bandaranayaka-Mudiyanselage, Eunah Kang, D. Scott Adams, Lowell S. Kappmeyer, Donald P. Knowles, Terry F. McElwain, James F. Evermann, Massaro W. Ueti, Glen A. Scoles, Stephen S. Lee and Travis C. McGuire J VET Diagn Invest published online 6 December 2013 DOI: 10.1177/1040638713511813 The online version of this article can be found at: http://vdi.sagepub.com/content/early/2013/12/06/1040638713511813
Published by: http://www.sagepublications.com
On behalf of:
Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.
Additional services and information for Journal of Veterinary Diagnostic Investigation can be found at: Email Alerts: http://vdi.sagepub.com/cgi/alerts Subscriptions: http://vdi.sagepub.com/subscriptions Reprints: http://www.sagepub.com/journalsReprints.nav Permissions: http://www.sagepub.com/journalsPermissions.nav
>> OnlineFirst Version of Record - Dec 6, 2013 What is This?
Downloaded from vdi.sagepub.com at Scientific library of Moscow State University on December 26, 2013
511813 research-article2013
VDIXXX10.1177/1040638713511813Chung et al.Improved performance of Anaplasma antibody cELISA
Article
Improved diagnostic performance of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5–glutathione S-transferase fusion protein as antigen
Journal of Veterinary Diagnostic Investigation XX(X) 1–11 © 2013 The Author(s) Reprints and permissions: sagepub.com/journalsPermissions.nav DOI: 10.1177/1040638713511813 jvdi.sagepub.com
Chungwon Chung,1 Carey Wilson, Chandima-Bandara Bandaranayaka-Mudiyanselage, Eunah Kang, D. Scott Adams, Lowell S. Kappmeyer, Donald P. Knowles, Terry F. McElwain, James F. Evermann, Massaro W. Ueti, Glen A. Scoles, Stephen S. Lee, Travis C. McGuire
Abstract. The current study tested the hypothesis that removal of maltose binding protein (MBP) from recombinant antigen used for plate coating would improve the specificity of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay (cELISA). The number of 358 sera with significant MBP antibody binding (≥30%I) in Anaplasmanegative herds was 139 (38.8%) when tested using the recombinant major surface protein 5 (rMSP5)-MBP cELISA without MBP adsorption. All but 8 of the MBP binders were rendered negative (
