ß 2014. Published by The Company of Biologists Ltd | Journal of Cell Science (2014) 127, 485–495 doi:10.1242/jcs.140194

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In vivo cell biology in zebrafish – providing insights into vertebrate development and disease

ABSTRACT Over the past decades, studies using zebrafish have significantly advanced our understanding of the cellular basis for development and human diseases. Zebrafish have rapidly developing transparent embryos that allow comprehensive imaging of embryogenesis combined with powerful genetic approaches. However, forward genetic screens in zebrafish have generated unanticipated findings that are mirrored by human genetic studies: disruption of genes implicated in basic cellular processes, such as protein secretion or cytoskeletal dynamics, causes discrete developmental or disease phenotypes. This is surprising because many processes that were assumed to be fundamental to the function and survival of all cell types appear instead to be regulated by cell-specific mechanisms. Such discoveries are facilitated by experiments in whole animals, where zebrafish provides an ideal model for visualization and manipulation of organelles and cellular processes in a live vertebrate. Here, we review well-characterized mutants and newly developed tools that underscore this notion. We focus on the secretory pathway and microtubule-based trafficking as illustrative examples of how studying cell biology in vivo using zebrafish has broadened our understanding of the role fundamental cellular processes play in embryogenesis and disease. KEY WORDS: Zebrafish, Protein secretion, Vesicular transport, Microtubule transport

Introduction

Classical cell biologists studied cells in organisms abundantly available in their environments, such as algae, plants and marine animals. As the field evolved, cultured cells became de rigueur, and thus in vitro studies have generated much of the modern cell biology lexicon. The genetic advantages and expanding tools for studying subcellular structures in the small transparent zebrafish embryo offer the opportunity for cell biologists to return to our roots and to address fundamental cell biological questions in the context of a whole organism. Forward genetic screens to identify genes underlying developmental events are a mainstay of zebrafish research. These screens have generated an extensive repertoire of mutants 1 Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1020, New York, NY 10029, USA. 2Department of Medicine/Division of Liver Diseases, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1020, New York, NY 10029, USA. 3Division of Genetic Medicine, Department of Medicine, and Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. 4Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany. 5Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1020, New York, NY 10029, USA.

*Author for correspondence ([email protected])

where a gene implicated in a basic cell biological process has been disrupted. Surprisingly, many of these mutants have specific phenotypes that involve only a few tissues or cell types. This is reminiscent of human genetic disorders such as mitochondriopathies (Schapira, 2006), ciliopathies (Hildebrandt et al., 2011) and diseases of protein trafficking (De Matteis and Luini, 2011) where a defect in a protein involved in a fundamental organelle function results in a discrete clinical syndrome. Similarly, researchers were surprised by the finding that many genes thought to be essential for the function or survival of cells in culture, and that were assumed to be ubiquitously expressed, were instead revealed to have spatio-temporally restricted expression patterns during zebrafish development. These findings led to the hypothesis that fundamental cellular processes are regulated by cell-type-specific mechanisms. The use of zebrafish and other animals – both invertebrate and vertebrate – (see Box 1) provides valuable insights into how basic cellular processes are regulated during development and how disrupting these processes can impact on embryogenesis. This is elegantly illustrated by a recent investigation into the formation of the notochord, which forms the embryonic axial skeleton (Ellis et al., 2013). Notochord cells appear hollow owing to a large cytoplasmic vacuole found to be a lysosomal-derived organelle generated by the endosomal trafficking machinery. Rab32a is a GTPase which, in cultured cells, has been found to be involved in mitochondrial dynamics (Alto et al., 2002; Bui et al., 2010), trafficking to autophagosomes (Hirota and Tanaka, 2009) and other lysosome-related functions (Bultema et al., 2012; Wasmeier et al., 2006), and, in zebrafish, Rab32a has been found to be essential for vacuole formation and hence for notochord development. This exemplifies how pairing traditional cell biological approaches with advances in microscopy, genetics and pharmacology in zebrafish leads to unprecedented understanding of how basic cellular processes drive specific developmental events. Here, we review the advantages of using zebrafish to study the intersection between cell biology, vertebrate development and pathology, and highlight some well-established imaging and genetic tools. We focus on the protein secretory pathway and microtubule-directed vesicular traffic to exemplify how merging cell biology, genetics and embryology has provided unique insight into how cells work in the context of tissues and organs in a living organism. The zebrafish toolbox – microscopes, genetics and drugs Zebrafish overview

Zebrafish produce large numbers of externally fertilized eggs, which rapidly develop into transparent embryos and progress to free-swimming feeding larvae within 5 days post fertilization (dpf). Gastrulation is complete within hours of fertilization and by 1 dpf, embryonic axes are established and neural development is 485

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Ana M. Vacaru1,2, Gokhan Unlu3, Marie Spitzner4, Marina Mione4, Ela W. Knapik3 and Kirsten C. Sadler1,2,5,*

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Zebrafish are the most widely used non-mammalian vertebrate organism, with thousands of laboratories devoted to zebrafish research worldwide. What distinguishes zebrafish from the fly (Drosophila melanogaster) and worm (Caenorhabditis elegans) – commonly used invertebrate organisms – is that, as a vertebrate, it shares properties with most organs found in mammals. Although zebrafish development is slower than that of invertebrates, it is considerably faster (5 days) than mice (19 days). As zebrafish development is external, like invertebrates, live embryos can be observed and manipulated; however, these same features make this system less than ideal for studies focused on in utero development or the transition from pre- to post-natal development. Finally, the high fecundity provides sufficient sample sizes to enable large-scale screening, and drug screens are facilitated by the ability to add compounds directly to their culture water. The zebrafish genome is diploid and has been fully sequenced, with annotation being underway on Ensembl, and the Sanger Center and other genome browsers, providing advantages over the tetraploid Xenopus genome. Genome duplication occurred during teleost development so that ,30% of zebrafish genes are duplicated (Howe et al., 2013; Woods et al., 2000; Woods et al., 2005). The advantage of this is that orthologs might have diverged in function and allow for emergence of specific phenotypes when one is targeted; however, there is the potential for duplicated genes that act redundantly, and hence phenotypes might not emerge unless both genes are targeted (Roest Crollius and Weissenbach, 2005). The transparency and accessibility of zebrafish embryos is a significant advantage for cell biology, allowing visualization of fluorescent proteins in vivo, as in worms. A low-power microscope is sufficient for observing organotypic defects or living embryos at a single-cell resolution. Thus, zebrafish provide an excellent system for live imaging in a whole vertebrate and are comparable to Xenopus in the ease of morpholino knockdown. However, the rapidity of the system and the repertoire of tools for manipulating gene function and expression have not yet achieved the same level that is available for flies and worms. Thus, there is value in each animal model and the strengths of each can be leveraged in comparative approaches to fully understand gene function.

underway. An exquisite map of cell division and movement during these early developmental events has been achieved by tracking individually labeled nuclei using advanced microscopy (Keller et al., 2008; Schmid et al., 2013). By the end of 2 dpf, organogenesis is underway throughout the embryo and, by 5 dpf, most organs carry out specialized functions. Although zebrafish have traditionally been used to study embryogenesis, areas such as behavior, pathology, infectious diseases and drug screening are actively investigated. Here, we focus on zebrafish tools used to study cell biology in vivo, with the aim to understand development and disease. Labeling subcellular structures in zebrafish

High-resolution studies of embryonic development and disease models require analysis of the behaviors of individual cells, subcellular structures and proteins in the context of an intact tissue or organism. Fluorescent proteins have revolutionized cell biology. However, the relative small size and dynamic nature of subcellular components present challenges for imaging. This is exacerbated by the cellular complexity of whole animals. Thus, in 486

contrast to the elegant studies describing organelle dynamics in cultured cells or yeast, relatively little is known about these processes in vertebrates. Developing a zebrafish toolkit that labels, tracks and measures intracellular structures comparable to that available in mammals is a work in progress. A recent and concerted effort by the zebrafish community and several companies has improved the library of antibodies recognizing zebrafish proteins, which are cataloged in the zebrafish model organism database (http://zfin. org). However, specific antibodies for many structures remain unavailable, and antibody staining does not allow live imaging, a strength of the zebrafish system. Developing transgenics (Kawakami, 2005; Kwan et al., 2007) and vital dyes provides a good alternative. Well-characterized fluorescent markers relevant to topics discussed here are listed in Table 1 and illustrated in Fig. 1F. Fig. 1 shows two examples of such transgenics: Tg(bact:GalT-GFP) fish express a portion of Galactose-1phosphate uridyl transferase (GALT) fused to GFP under the semi-ubiquitous b-actin promoter (Gerhart et al., 2012) to enable imaging of the trans-Golgi in the developing embryo using both a low-resolution fluorescence stereomicroscope (Fig. 1A–C) and confocal imaging (Fig. 1D). The second, Tg(ef1a:dclk-GFP) line (Tran et al., 2012), labels microtubules with GFP and, when used in combination with the vesicle marker clathrin fused to dsRed (M.S. and M.M., unpublished data; Fig. 1E), it provides a realtime view of microtubule-based vesicular transport. Fluorescent vital dyes also allow for imaging subcellular structures in vivo. BODIPY, Alizarin Red, quantum dots and dyes, such as MitoTracker or LysoTracker, are versatile counterstains to visualize dynamic cellular processes in live fish (Cooper et al., 2005; He et al., 2009; Ko¨ster and Fraser, 2006; Rieger et al., 2005; Zhang et al., 2008). In combination, transgenics and dyes provide a microscopic view of intracellular processes and a macroscopic view of how disrupting basic cellular processes interrupt development or cause disease. Imaging

Many zebrafish imaging studies are carried out using microscopes that are available to most researchers, whereas an in-depth analysis of dynamic movements or morphometric analyses of subcellular structures requires the advanced microscopy techniques limited to researchers who have specialized training and access to facilities with these capabilities. Low-resolution epifluorescent and stereomicroscopes can be used to detect gross changes in localization or intensity of many markers, and standard confocal microscopy can image cells that are close to the surface of live embryos but imaging deeper cells requires histological sections. Unprecedented views of how cells move individually and in concert in a developing embryo have been provided using light-sheet microscopy to track individual nuclei labeled with fluorescently-tagged histones (Huisken and Stainier, 2009; Keller et al., 2008). Selective plane illumination microscopy has provided a three-dimensional view of endodermal cell movement in older live embryos (Schmid et al., 2013). Multiphoton imaging has been used to investigate how neuronal circuits assemble during retinal development by tracking dynamic changes in cell structure and connectivity (Williams et al., 2013), and super-resolution microscopy has been used to image a single receptor molecule uncovering the role for receptor clustering in the antiviral immune response (Gabor et al., 2013). Many more spectacular zebrafish images are being generated as imaging technologies continue to improve.

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Box 1. Comparative analysis of common model organisms

Journal of Cell Science (2014) 127, 485–495 doi:10.1242/jcs.140194

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Table 1. List of markers and tools of intracellular and extracellular structures used in vivo in zebrafish Cellular structure or process

Tool type

Tool name

Application

References

Nucleus

Vital dye

Acridine orange

Visualize the nuclei of dying cells

Chromosomes

Transgenic Transgenic Transgenic Transgenic Transgenic

H2B–mRFP H2B–EGFP NLS–EGFP NLS–mCherry H2afz–GFP

Visualize nuclei in vivo Visualize nuclei in vivo Visualize the nuclei in vivo Visualize the nuclei in vivo Visualize chromosomes during mitosis and track single nuclei during early development

Paquet et al., 2009; Peri and Nu¨sslein-Volhard, 2008 Distel et al., 2011 Keller et al., 2008 Kim et al., 2011 Lam et al., 2010 Laguerre et al., 2009

Cytoplasm

Vital dye

Secretion

Transgenic

BODIPY TR methyl ester dye Dbp–EGFP

Endoplasmic reticulum stress

Transgenic

huORFZ

Golgi apparatus Endosomes

Transgenic Vital dye Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic Transgenic

Transgenic Transgenic

GalT–GFP F4-64 Rab3–YFP Rab5c–GFP Rab5c-Q81L–mCherry Rab5c-S36N–mCherry Rab7–GFP Rab7-Q67L–mCherry Rab7-T22N–mCherry Rab11a–GFP Rab11a-Q70L–mCherry Rab11a-S25N-mCherry Rab32a–GFP Rab32a-T27N–GFP Rab38b-T23N-GFP Annexin–Cy5 GFP–CaaX (mem–GFP) Mem–CFP Dclk–GFP

Transgenic

Plasma membrane

Microtubules and motor proteins

Lysosomes

Transgenic Vital dye Transgenic

Cooper et al., 2005 Monitoring glycoprotein secretion from hepatocytes huORFchop–GFP [expresses GFP fused to the upstream open reading frame (uORF) from the 5’UTR of the human CHOP ]* Trans-Golgi marker Endosomal marker Secretory endosomal marker Early endosomal marker Constitutively active form of Rab5 Dominant-negative form of Rab5 Late endosomal marker Constitutively active form of Rab7 Dominant-negative form of Rab7 Recycling endosomal marker Constitutively active form of Rab11 Dominant-negative form of Rab11 Endosomal marker Dominant-negative form of Rab32 Dominant-negative form of Rab38 Visualize early membrane of apoptotic cells Plasma membrane marker

Xie et al., 2010 Lee et al., 2011

Gerhart et al., 2012 Fischer-Parton et al., 2000 Campbell et al., 2007 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Clark et al., 2011 Ellis et al., 2013 Ellis et al., 2013 Ellis et al., 2013 Peri and Nu¨sslein-Volhard, 2008 Ellis et al., 2013

Plasma membrane marker Microtubule marker

Distel et al., 2011 Tran et al., 2012

Tau–GFP

Marker for plus-end of microtubules in axons

Transgenic Transgenic

a-tubulin–GFP Kif17–GFP

Microtubule marker Marker for plus-end directed vesicle trafficking

Transgenic

EB1–GFP

Transgenic

EB3–GFP

Transgenic

Lamp2–GFP

Labels microtubules plus-end to visualize growth direction Labels microtubules plus-end to visualize growth direction Lysosomal marker

Yanicostas et al., 2009; Yoshida et al., 2002 Asakawa and Kawakami, 2010 Bader et al., 2012; Insinna et al., 2009 Tran et al., 2012 Tran et al., 2012 Ellis et al., 2013

Genetics

The zebrafish genome has been sequenced and annotated, and most zebrafish genes are highly conserved in mammals, with a zebrafish ortholog identified for ,70% of human genes (Howe et al., 2013). Transgenesis and forward genetic screens are important widely used methods in zebrafish, and recent advances in reverse genetic approaches are having a major impact on the field. Box 2 details methods of generating transgenics and targeted mutagenesis using TALENs and the Crispr/Cas systems. Forward genetic screens allow the unbiased discovery of genes that contribute to a specific phenotype and have generated thousands of mutants in the zebrafish genome. One drawback, however, is that generating and maintaining stable lines can be laborious and another is that the genome duplication that occurred during teleost evolution

(Postlethwait et al., 1998) can generate genes that act redundantly, necessitating that both are targeted before revealing a phenotype. In addition, maternal stores of mRNA and proteins can delay the emergence of mutant phenotypes until after these are exhausted, and thus mutant phenotypes often reflect the impact of gene loss on later developmental stages. Morpholinos provide a complementary approach, whereby oligonucleotides that are injected into the fertilized egg block target mRNA translation or splicing. Although the use of morpholinos is plagued by fears of off-target effects and transient effects, translation-blocking morpholinos deplete proteins derived from both maternal and zygotic mRNA, so that the effects of target gene loss on early embryonic events can be studied. Additionally, by titrating the amount of morpholino 487

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*This regulates GFP translation to reflect endogenous Chop levels and monitor the ER stress response. GFP is found in neural tissues during ER and thermal stress.

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A

2 dpf

B

D

Tg(bact:GalT-GFP) DAPI

Hepatocytes 10 µm Tg(bact:GalT-GFP) 4 dpf Tg(ef1a:dclk-GFP) E clathrin-DS-Red

Fig. 1. Fluorescent protein markers of subcellular structures in zebrafish. (A–C) Live 2 dpf Tg(bact:GalTGFP) embryo highlighting the trans-Golgi complex. The boxed region in B is magnified in the C. (D) Confocal projection through a cryosection of the liver from 5 dpf Tg(bact:GalT-GFP) larva. Nuclei are stained with DAPI (gray). (E) Muscle in a live 1 dpf Tg(ef1a:dclk-GFP) embryo with GFP-tagged microtubule-associated protein (DCK) and vesicles marked with clathrin-DS-Red (magenta). (F) Zebrafish fluorescent-protein-tagged organelle markers that can be expressed with transgenics. PM, plasma membrane; LE, late endosomes; EE, early endosomes; RE, recycling endosomes.

200 µm

C

200 µm

Muscle cells 1 dpf

10 µm

GFP–CaaX mem–CFP

F

PM

COPII

Rab7–GFP

LE

ERGIC

Rab5–GFP

EE E E

GalT–EGFP Nucleus

Golgi

NLS–cherry h2afz–mRFP NLS–EGFP

Rab11–GFP

RE Lamp2–GFP

Lysosome

COPI ER

Microtubules

injected, the degree of knockdown can be finely tuned. This has proved particularly useful for our work to model one type of congenital disorder of glycosylation (CDG), which, in humans is caused by a hypomorphic mutation in one of the genes required for N-linked protein glycosylation (Freeze, 2007). Homozygous null mutations of these genes are lethal in mammals (Thiel and Ko¨rner, 2011) and injecting high morpholino concentrations causes severe embryonic phenotypes and high mortality in zebrafish (Chu et al., 2013; Cline et al., 2012). However, fine-tuning of the knockdown was facilitated by injecting lower morpholino concentrations so that residual enzyme activity in the zebrafish morphants matched that measured in samples from patients, improving survival and revealing novel phenotypes (Chu et al., 2013; Cline et al., 2012). Although the breadth and speed of zebrafish genetic approaches do not match those available in invertebrates, they are accomplished at a fraction of the costs of, and with sample sizes exceeding, typical rodent experiments (see Box 1). 488

Drugs

Zebrafish have a rich history in toxicology research, as compounds can be simply added to their water. For example, zebrafish are proving useful for alcohol research (Jang et al., 2012; Monk et al., 2013; North et al., 2010; Passeri et al., 2009; Tsedensodnom et al., 2013; Yin et al., 2012). By using transgenic zebrafish expressing a GFP-tagged secreted glycoprotein in hepatocytes (Howarth et al., 2013; Tsedensodnom et al., 2013; Xie et al., 2010), and zebrafish that express fluorescent protein markers of the hepatocyte secretory organelles and of other cells in the liver (Yin et al., 2012), the mechanisms by which alcohol and other drugs cause organ-specific and organelle-mediated toxicity can be uniquely addressed. Moreover, large-scale drug screens exploit the ease of treating zebrafish with drugs (Peterson and Macrae, 2012) and have identified compounds modulating processes ranging from metabolism (Nath et al., 2013) to sleep (Rihel and Schier, 2012).

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GFP–α–tubulin dclk–GFP Tau–GFP Kif17–GFP EB1–GFP EB2–GFP

Box 2. Genetic approaches – a summary Transgenics are a common means to overexpress genes in zebrafish and, now, both transient knockdown by morpholinos and gene targeting are routine methods to induce gene loss. Random targeting of a transgene and promoter in the genome is facilitated using the tol2 transposon (Suster et al., 2009). Cellspecific promoters are available for many cell types to direct transgene expression and bacterial artificial chromosome recombineering can recapitulate endogenous gene expression patterns and levels; the heat shock promoter (hsp70) is also a widely used inducible system (Halloran et al., 2000). Multiple lines of fish expressing the same transgene in different tissues can be quickly generated by crossing fish in which the transgene is under the UAS promoter, to lines that express the Gal4 transcriptional activator under a tissue-specific promoter (Distel et al., 2009; Halpern et al., 2008). Chemical and gene-breaking transposons (http://zfishbook.org/) have generated a large library of non-targeted mutants. Homologous recombination to generate targeted gene mutations has eluded the zebrafish community for years. However, use of TALENs (transcription activator-like effector nucleases) (Bedell et al., 2012; Huang et al., 2011; Sander et al., 2011) and the type II prokaryotic CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system (Hwang et al., 2013; Jao et al., 2013; Xiao et al., 2013) has recently been successful in specific gene targeting to generate germ-line mutants. These systems use chimeric nucleases composed of programmable sequence-specific DNA-binding modules linked to nonspecific DNA nucleases to induce double-stranded DNA breaks that are inefficiently repaired by error-prone non-homologous endjoining or homology-directed repair (Gaj et al., 2013). TALEN pairs that have been engineered by a zebrafish consortium are commercially available from http://www.addgene.com for many genes. Resources for TALEN design include https://groups.google. com/group/talengineering, http://www.addgene.org/talengineering/ TALENzebrafish/ and http://www.TALengineering.org The CRISPR/Cas system introduces single nucleotide substitutions, insertions and deletions ranging from 1 to 20 bp. The relative ease of synthesis makes the CRISPR/Cas systems an attractive approach for genome editing (see Hwang et al., 2013; http:// www.addgene.org; http://www.crispr-cas.org). It is anticipated that within the near future, nearly all protein-coding genes in zebrafish will have been disrupted by either forward genetic screens or one of these reverse genetic approaches.

The protein secretory pathway

The secretory pathway generates, trafficks and processes proteins destined for the extracellular space or the plasma membrane. It comprises the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment (ERGIC), the Golgi complex and the vesicles that carry cargo between them (Fig. 2). Protein synthesis and glycosylation occur in the ER and Golgi, respectively. The coat protein II (COPII) complex facilitates cargo selection, vesicle formation and anterograde trafficking from the ER to the Golgi, whereas retrograde transport occurs in COPI vesicles (Fig. 1F; Fig. 2). Diverse protein complexes function at each step of this pathway to recruit Rab GTPases and SNARE proteins, which direct and tether vesicles to target organelles and facilitate membrane fusion. Work in unicellular organisms and cultured cells created an assumption that protein secretion is uniformly regulated across cell types. Studies in human, zebrafish and other vertebrates, however, revealed that this pathway is regulated in a spatio-temporal and

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paralog-specific manner (Melville and Knapik, 2011; Unlu et al., 2013). Although all cells secrete proteins, some – including Bcells, chondrocytes, hepatocytes and the endocrine and exocrine pancreatic cells – are considered ‘professional’ secretory cells and these cells were assumed to be particularly sensitive to secretory pathway disruption. Zebrafish mutants in secretory pathway genes both supported and refuted this hypothesis: some mutants show phenotypes in most highly secretory cells, whereas others have phenotypes that are restricted to only a subset of cells. The emerging view is that the secretory machinery is integral to morphogenesis and organ function in a cell-specific fashion. The availability of zebrafish genetic mutants and fluorescent in vivo reporters provides novel insight into organismal functions of the secretory pathway. The effects of secretory pathway disruption relevant to development and disease are discussed below. Developmental consequences of secretory pathway disruption

The COPII complex comprises the Sar1 GTPase, Sec23–Sec24 dimers of the inner coat, and Sec13–Sec31 heterotetramers of the outer coat (Fig. 2; Kaiser and Schekman, 1990; Novick et al., 1980). Zebrafish mutants of sec23a (crusher) and sec24d (bulldog) develop craniofacial dysmorphology, kinked pectoral fins and short body length (Lang et al., 2006; Sarmah et al., 2010). These are attributed to a failure of extracellular matrix (ECM) secretion during chondrocyte differentiation. Sec23A- and Sec24D-deficient animals fail to export collagen and other Nglycosylated proteins from the chondrocyte ER, arresting differentiation and ultimately causing cell death (Lang et al., 2006; Sarmah et al., 2010; Unlu et al., 2013), whereas collagen secretion and skeletal development are intact upon depletion of the close paralog Sec24C (Sarmah et al., 2010). Sec23B mutations in humans and zebrafish disrupt erythropoesis (Bianchi et al., 2009; Schwarz et al., 2009), a different phenotype from the chondrocyte defects observed in crusher and bulldog mutants. These COPII phenotypes are distinct from the dwarf mutants sneezy, happy and dopey, which disrupt genes that encode the a, b and b9 subunits of the COPI complex, respectively (Fig. 2), which are characterized by defects in notochord and melanosome formation (Coutinho et al., 2004). These data suggest that although COPI and COPII are required for efficient secretion and membrane recycling in all cells, loss of specific members of each complex have profound and disparate effects on a subset of cells. Mutations in individual COPII components cause an array of phenotypes in highly secretory cell types in organs such as cartilage, notochord, eye and gut (Niu et al., 2012; Schmidt et al., 2013; Townley et al., 2008; Townley et al., 2012), and in erythrocytes (Bianchi et al., 2009; Schwarz et al., 2009; Unlu et al., 2013), whereas cells that depend on vacuole formation are most sensitive to COPI depletion. So how do transcriptional regulatory mechanisms direct the secretory pathway to assure a timely availability of cargo-specific coats? A large-scale screen in zebrafish identified the feelgood mutant, which carries a missense variant in the creb3L2 gene (Driever et al., 1996; Knapik, 2000; Neuhauss et al., 1996) – the first known transcription factor that regulates availability of the COPII components sec24d and sec23a, but not sec24c (Melville et al., 2011). Similarities between feelgood, crusher and bulldog mutant phenotypes suggest that a ‘secretory module’ consisting of Creb3L2–Sec23A–Sec24D specializes in procollagen secretion. Given that zebrafish depleted of Sec24C do not manifest skeletal dysmorphology and the gene is not a target of Creb3L2, it is 489

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SEC13-SEC31 (?) Sec13 (sec13sq198), sec31a (mo)

SEC23B (CDAII) Sec23B (mo)

SEC24D (?) Sec24D (bulldog)

SEC23A (CLSD) Sec23A (crusher)

SEC24B (NTD) Sec24B (?)

TRAPPC11 (LGMD) Trappc11 (foie gras) TRAPPC2 (SEDT) Trappc2 (?) TRAPPC9 (ID) Trappc9 (?)

SAR1B (CMRD) Sar1B (mo)

SE9C24C (?) Sec24C (mo)

TRAPP complex

COPII vesicle

Exocytic vesicle

COPI

Fig. 2. Secretory pathway components studied in zebrafish and implicated in human diseases. Secretory pathway proteins implicated in human disease are shown in black with corresponding zebrafish tools to study these in red. NTD, neural tube defects; CLSD, cranio-lenticular-sutural dysplasia; CDAII: congenital dyserythropoietic anemia II; CMRD, chylomicron retention disease; LGMD, limb girdle muscular dystrophy; SEDL, X-linked spondyloepiphyseal dysplasia tarda; ID, intellectual disability.

Golgi

Nucleus s

ER COPI complex

Plasma

βCOP (?) βCOP (happy)

β⬘COP (?) β⬘COP (dopey)

αCOP (?) COP (sneezy)

likely that other cargo-specific secretory modules regulate sec24c and other genes in this pathway. Future studies in zebrafish and other animal models will be needed to crack the code of physiologically relevant, cargo-specific secretory networks. Diseases caused by secretory pathway disruption in zebrafish and humans

Several human syndromes are associated with secretory pathway defects (De Matteis and Luini, 2011), some of which are recapitulated in mutations in orthologous zebrafish genes (Fig. 2). The concurrent identification of crusher/sec23a mutants in zebrafish and patients with SEC23A/cranio-lenticulo-sutural dysplasia (CLSD) variants (Boyadjiev et al., 2006; Lang et al., 2006) provides an excellent example of convergence between human genetics and zebrafish developmental biology to uncover physiological ramifications caused by disrupting basic cell biological processes. Both crusher mutants and CLSD patients present with craniofacial dysmorphology and axial skeleton defects attributed to backlog of ECM proteins in the ER (Boyadjiev et al., 2006; Lang et al., 2006). The closely related SEC23B gene is mutated in congenital dyserythropoietic anemia type II patients who have multinucleated erythroblasts in bone marrow, a phenotype recapitulated in zebrafish sec23b morphants (Bianchi et al., 2009; Schwarz et al., 2009). It is unclear why mutations in SEC23A and SEC23B paralogs, which differ only by an 18 amino acid stretch, cause such different phenotypes. One possibility is that spatio-temporal differences in expression confer cell-specific functions of some COPII complex genes. However, as sar1a and sar1b are ubiquitously expressed early in development and become enriched in distinct tissues later on (E.W.K., unpublished observations), it is unlikely that their gene expression pattern is solely responsible for the divergent phenotypes observed in these mutants. The craniofacial phenotypes of COPII mutants suggest that chondrocytes are highly sensitive to ECM secretory defects. This predicts that other manipulations that block the secretory pathway 490

would also cause craniofacial dysmorphology. This, however, is not supported by data from zebrafish or humans when factors functioning at other steps in the secretory pathway are depleted. The transport protein particle (TRAPP) complex tethers ERderived vesicles to the cis-Golgi membrane (Fig. 2 and Sacher et al., 2008). Fibroblasts from patients with TRAPPC11 or TRAPPC2 mutations (Bo¨gershausen et al., 2013; Scrivens et al., 2009), and cultured cells depleted of TRAPPC11 (Scrivens et al., 2011; Wendler et al., 2010) display Golgi fragmentation and secretory protein retention. However, when these proteins are depleted in whole organisms their cell-specific roles are uncovered: patients with TRAPPC2 mutation develop spondyloepiphyseal dysplasia tarda (SEDT) distinguished by skeletal defects, short stature and microcephaly (Gedeon et al., 1999; Huson et al., 1993). This is recapitulated in trappc2 zebrafish morphants, which have a short trunk and microcephaly (A. M. Vacaru and K. C. Sadler, unpublished). The short stature and/or trunk phenotype might reflect a defect in chondrocyte formation or in ECM deposition, as in COPII mutants, but patients and zebrafish with TRAPPC11 mutation present very differently. TRAPPC11 mutation in humans causes myopathy, intellectual impairment and hyperkinetic movements (Bo¨gershausen et al., 2013). Interstingly, zebrafish foie gras (foigr) mutants carrying a mutagenic viral insertion in the trappc11 gene display a different phenotype from other models of TRAPP complex disruption: they develop fatty liver, hepatomegaly, smaller gut and jaw, and fin defects (Cinaroglu et al., 2011; Sadler et al., 2005). The foigr/trappc11 fatty liver phenotype is partially attributed to activation of the unfolded protein response (Cinaroglu et al., 2011); however, neither mammalian cultured cells that are depleted of TRAPPC11 nor trappc2 morphants induce this response (A.M.V. and K.C.S., unpublished observations), highlighting the utility of in vivo cell biology studies in vertebrates. The unique phenotypes that differentiate TRAPP complex disruption from COPII or COPI mutations indicate that a global

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block in protein secretion is not the only mechanism that underlies their associated phenotypes. Moreover, although depleting individual TRAPP or COP complex factors has similar effects in isolated cells, the physiological consequences could not be predicted without use of whole animals. These findings point to cell- and developmental-specific roles for each gene involved in protein secretion and underscore the need for comparative whole animal models to decipher the cellular and physiological functions of this pathway. Microtubule highways in vesicular transport

Microtubules serve as tracks for organelles and vesicle movement (Figs 1, 3), an especially crucial task for the long axons of nerve cells. Microtubules are formed by a-tubulin–b-tubulin heterodimers that generate a polarized structure – the primary determinant of motor protein directional movement. The fast growing plus-end (b-tubulin capped) and a degrading minus-end (a-tubulin capped) have centrosomes that serve as microtubule-organizing centers from which microtubules undergo assembly and disassembly (Wade, 2007). Zebrafish tools to study microtubules

Several transgenic zebrafish lines have been developed to image microtubules in vivo (Table 1; Figs 1, 3). A GAL4–UAS system has been used to spatially and temporally express GFP-tagged a-tubulin (Asakawa and Kawakami, 2010). GFP-tagged doublecortin-like kinase (York et al., 2012), a ubiquitously expressed microtubuleassociated protein, uncovered the dynamics and polarity of microtubules in early-stage embryos (Tran et al., 2012). Markers of the growing plus-end of microtubules, including GFP-tagged Tau (Yanicostas et al., 2009; Yoshida et al., 2002) and the plus-end microtubule components Eb1 and Eb3 (Tran et al., 2012), have been combined with other lines that express markers of specific vesicle

populations (Fig. 1C) for live imaging of vesicular transport along microtubules in whole animals. Cargos are transported along microtubules by members of two motor protein superfamilies, the kinesins (Kifs) and the dyneins (Fig. 3). Although Kifs are cargo-specific, only cytoplasmic dynein1 (Dync1) is involved in directed microtubule transport of cargos (Roberts et al., 2013). Dync1 is a multi-protein complex that achieves specificity and versatility through distinct subunits and interaction with regulatory proteins, including dynactin (Karki and Holzbaur, 1999; King and Schroer, 2000; King, 2000). Like vesicle trafficking in the secretory pathway, cargo–motor interactions are regulated by Rab GTPases which serve to regulate, connect and attach cargo to microtubule motors. Rab3A connects vesicles to Kif1Bb and Kif1A in neuronal cells (Niwa et al., 2008), whereas Rab11A regulates endosomal trafficking events through interaction with Kif3B (Schonteich et al., 2008) and Rab7 controls late endosomal transport (Jordens et al., 2001). The available markers for Rabs and their dominant-negative versions in zebrafish are included in Table 1. GFP-tagged Rabs in zebrafish colocalize to vesicles with lipophilic dye FM4-64 (Fischer-Parton et al., 2000), and experiments using this system have shown that different Rabs associate with different types of vesicles (Clark et al., 2011). This analysis was aided by an automated tracking program (https://pantherfile.uwm.edu/cohena/ www/rabtools.html), which enabled the in-depth analysis of recycling endosomes and their intracellular dynamics, an unprecedented feat in whole organisms. Collectively, work on microtubule-based transport in zebrafish has led to several important conclusions: (1) microtubule-based cargo transport shapes embryonic development from fertilization onwards; (2) different motor proteins control transport in different cell compartments – for instance, Kif17 in the photoreceptor outer

Cargo

RAB11 Rab11 (mCherry–Rab11DN, mCherry–Rab11CA)

Minus-end

Cargo

KIF18 (CMT2A) Kif1b (kif1bst43)

RAB7 (CMT2B) Rab7 (mCherry–Rab7DN, mCherry–Rab7CA)

Cargo

Cargo

Cargo

Plus-end

Key

Dynein (NDD) Dync1h1 (cnb, mikreoko, ale oko)

Microtubule

Dynein

Rab3

Kif1B

Dynactin

Rab7

Kif3 Kif5

DENN/MAD RIF11

Rab11A Rab27

Fig. 3. Microtubules, motor proteins and motor- and microtubule-binding proteins studied in zebrafish and involved in human diseases. Microtubule associated proteins implicated in human disease are shown in black with corresponding zebrafish tools to study these are in red. HSP, hereditary spastic paraplegia; NDD, neurodegenerative disease.

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KIF5 (HSP) Kif5 (?)

segment and Kif3 in the cytoplasm; and (3) zebrafish Rab homologs recapitulate the behavior of their counterparts in mammalian cells. Thus, the expanding set of tools in zebrafish is enabling organism-wide functional analysis of proteins involved in directed vesicular transport. Off-track – microtubule-based diseases in humans and zebrafish

Although most cells rely on microtubules for transporting their cargo, disruption of this process profoundly affects the central and peripheral nervous system due to the long-distance that cargos travel from cell bodies to axonal tips. Alzheimer, Huntington disease (HD) and Charcot–Marie–Tooth disease (CMT) (De Vos et al., 2008) are some neurodegenerative diseases (NDDs) caused by defects in vesicular transport. Fig. 3 illustrates the orthologous genes underlying NDDs in humans and mutant phenotypes in zebrafish. CMT type 2A is an axonal sensorimotor neuropathy characterized by severe peripheral muscle weakness and atrophy induced by loss of function mutations in the kinesin-3 family member, Kif1B (Zhao et al., 2001). In zebrafish, the point mutation kif1bst43 affects the microtubule interaction site and has been used to study kif1b functions in the developing central nervous system. The use of chimeric embryos, generated by transplanting kif1b-deficient neuronal cells into wild-type hosts (and vice versa), has revealed that there is a reduced growth potential in mutant neuronal axons (Lyons et al., 2009). Thus, kif1b is required in a cell autonomous manner for axonal development in the central and peripheral nervous system. Shprintzen–Goldberg syndrome is characterized by central and enteric nervous system defects and caused by homozygous mutations of the Kif1-binding protein Kbp (Brooks et al., 2005). The role of kbp in axonal outgrowth and maintenance, microtubule organization and localization of axonal mitochondria and vesicles was elucidated using kbpst23 zebrafish line that expresses a mutated version of kbp (Lyons et al., 2008). These studies uncovered a reduced number of axons in the enteric nervous system of kbpst23 mutants, providing a new animal model for this syndrome. Several mutations in cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) are linked to hereditary motor neuropathies including CMT and spinal muscular atrophy with lower extremity predominance (Harms et al., 2012; Weedon et al., 2011). Mutations in axonemal dynein or cytoplasmic dynein 2 have been linked to a large number of ciliopathies (Leigh et al., 2009; Huber and Cormier-Daire, 2012). Zebrafish morphants for axonemal dyneins are used to study left–right asymmetry (Kawakami et al., 2005); (Essner et al., 2005), and morphants of cytoplasmic dynein 2 subunits revealed ciliary abnormalities in zebrafish kidney, eye and nose (Krock et al., 2009). Although the mechanism of pathogenesis caused by dync1h1 mutations remains unclear, experiments in mice have suggested that there are defects in axonal transport of mitochondria (Eschbach et al., 2013). This is also reflected in the zebrafish dync1h1 (cannonball) mutant, which displays abnormal organelle positioning and accumulation of Golgi-associated vesicles in the inner segment of the retina (Insinna et al., 2010) and Schwann cell deficient myelination (Langworthy and Appel, 2012), a symptom common to NDDs. Additionally, dynactin mutations have been found in patients with amyotrophic lateral sclerosis and Perry syndrome (Stockmann et al., 2013). Death of sensory neurons and axonal degeneration is also reported in zebrafish with morpholino knockdown of dctn1a, dctn1b and dctn2a (Insinna et al., 2010) and the mutants dctn1a (mikre oko) 492

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(Tsujikawa et al., 2007) and dctn2 (ale oko) (Jing and Malicki, 2009). This suggests that depletion of Dync1 or Dync2 has a dose-dependent effect on photoreceptor cell organization and on vesicle transport. Mutations in Rab GTPases can also lead to NDDs. It has been shown that Rab11-dependent vesicle formation and transport is influenced by the huntingtin (Htt) protein, which causes HD (Li et al., 2009a; Li et al., 2009b). Zebrafish expressing a GFP-tagged Htt mutant protein (Williams et al., 2008) are a valuable tool to identify the Rabs and other factors that are required for Htt trafficking. The transgenics described above with dominantnegative and constitutively active versions of fluorescently tagged Rab5, Rab7 and Rab11 proteins (Clark et al., 2011; Ellis et al., 2013) will be valuable systems for dissecting specific contributions of Dync1, dynactin and Rab GTPases in NDDs. All cells are not created equal – conclusions and future directions

Identifying novel genes that regulate embryogenesis is a common aim of zebrafish forward genetic screens, which continue to supply libraries of mutants with disease-related phenotypes. Historically, researchers have focused on those mutants caused by disrupting genes encoding transcription factors or signaling molecules that are thought to serve key roles in directing specific developmental processes. However, similar phenotypes result from mutations in genes that serve basic cellular functions. These have been largely overlooked. The vacuolar sorting protein 18 (vps18a) mutant serves to illustrate this point: VPS18 is required for endosomal and lysosomal trafficking in yeast (Poupon et al., 2003) but in zebrafish, vps18a mutation causes specific defects in the hepatobiliary system, notochord and melanocytes (Ellis et al., 2013; Maldonado et al., 2006; Sadler et al., 2005). This recurring theme – whereby a gene is assumed to serve a universal and essential cellular function based on studies in yeast or isolated cells but instead is revealed to play cell-specific roles during development or in a pathology – is an important and largely underexplored topic in cell biology, and can be addressed by using zebrafish. Other lines of evidence that challenge the widely held assumption that genes that carry out basic cellular functions must be ubiquitously expressed and function equivalently in all cells include an in situ screen to document the expression patterns of the zebrafish transcriptome during development, with many such genes found to be expressed in a spatio-temporally specific fashion. For instance, the zebrafish orthologs of members of the heterotrimeric Sec61 translocon complex – sec61al1, sec61al2, sec61b and sec61 – which threads newly synthesized proteins from the ribosome to ER lumen, all have distinct in situ expression patterns [Thisse, B. and Thisse, C. (2004) Fast Release Clones: A High Throughput Expression Analysis. ZFIN Direct Data Submission (http://zfin.org/)]. Interestingly, mutation in sec61al1 causes defects in the jaw (Nissen et al., 2006), liver and gut (K.C.S., unpublished observations) and brain ventricle laterality (Doll et al., 2011), corresponding to the organs where sec61al1 is highly expressed, indicating tissue-specific roles for this translocon component. Screens typically yield more mutants than can be examined in detail. Although much has been learned from mutants that have obvious ties to well-studied transcriptional or signaling networks, we propose that focusing instead on those mutants resulting from defects in genes that have been previously classified as fundamental to cellular homeostasis are equally valuable because such mutants can clarify how cells are integrated into the function and formation of the organism.

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In summary, in vivo cell biology in zebrafish is uncovering tissue- and organ-specific functions for genes that previously have been assumed to serve the same function across cell types. The multicolor imaging tools and multiple genetic approaches allow zebrafish researchers to address both developmental and disease-related questions pertinent to cell biology. Because of these advances, we are coming full circle back to the topics that were of interest to the cell biology founders who pioneered studies of cells in situ. Acknowledgements We are grateful to Jaime Chu and Michel Bagnat for critical reading of the manuscript.

Competing interests The authors declare no competing interests.

Funding The work of our laboratories is supported by the Association for International Cancer Research (to M.M.); the National Institutes of Health (to K.C.S. and E.W.K.); the Zebrafish Initiative of the Vanderbilt University Academic Venture Capital Fund (to E.W.K.); and the Vanderbilt International Scholar Program (to G.U.). Deposited in PMC for release after 12 months.

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