Graefes Arch Clin Exp Ophthalmol DOI 10.1007/s00417-013-2508-z
RETINAL DISORDERS
Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment Sirpa Loukovaara & Kaisa Lehti & Alexandra Robciuc & Timo Pessi & Juha M. Holopainen & Katri Koli & Ilkka Immonen & Jorma Keski-Oja
Received: 4 March 2013 / Revised: 8 August 2013 / Accepted: 21 October 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Purpose To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker. Methods Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker
This study was partially presented at the XXVIIIth Meeting of Club Jules Gonin, in Reykjavik, Iceland, June 23rd 2012. S. Loukovaara (*) : I. Immonen Unit of Vitreoretinal Surgery, Department of Ophthalmology, Helsinki University Central Hospital, Haartmaninkatu 4 C, 00290 Helsinki, Finland e-mail: [email protected] K. Lehti Genome-Scale Biology, Haartman Institute, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland K. Koli : J. Keski-Oja Molecular Cancer Biology, Haartman Institute, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland A. Robciuc : J. M. Holopainen Helsinki Eye Laboratory, Department of Ophthalmology, University of Helsinki, Helsinki, Finland A. Robciuc National Institute of Health and Welfare, Institute for Molecular Medicine, Biomedicum Helsinki, Helsinki, Finland T. Pessi Datawell Oy, Espoo, Finland
(control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography. Results The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all p
RETINAL DISORDERS
Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment Sirpa Loukovaara & Kaisa Lehti & Alexandra Robciuc & Timo Pessi & Juha M. Holopainen & Katri Koli & Ilkka Immonen & Jorma Keski-Oja
Received: 4 March 2013 / Revised: 8 August 2013 / Accepted: 21 October 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Purpose To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker. Methods Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker
This study was partially presented at the XXVIIIth Meeting of Club Jules Gonin, in Reykjavik, Iceland, June 23rd 2012. S. Loukovaara (*) : I. Immonen Unit of Vitreoretinal Surgery, Department of Ophthalmology, Helsinki University Central Hospital, Haartmaninkatu 4 C, 00290 Helsinki, Finland e-mail: [email protected] K. Lehti Genome-Scale Biology, Haartman Institute, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland K. Koli : J. Keski-Oja Molecular Cancer Biology, Haartman Institute, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland A. Robciuc : J. M. Holopainen Helsinki Eye Laboratory, Department of Ophthalmology, University of Helsinki, Helsinki, Finland A. Robciuc National Institute of Health and Welfare, Institute for Molecular Medicine, Biomedicum Helsinki, Helsinki, Finland T. Pessi Datawell Oy, Espoo, Finland
(control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography. Results The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all p
