J. (:oMt’.
P.lTrr.
1979.
\.OL.
IXFECTION
lOi
89.
OF TRACHEAL ORGAK OVINE ADESOVIRUS
CULTURES TYPE 2
\YITH
B)
R.
S.
F.
CAMPBELL,*
:lnimn/
A. RAE,
J.
Diseaser Research Association.
Moredun
31.
SHARP Imhte.
and
SMITH
It:.
ICdinbur~qh, Scotland
INTRODUCTION
t:ultivation of viruses in tracheal explants has become a standard research method and various aspects of the growth and pathogenesis of a range of human and animal viruses have been described (Campbell, Thompson, Leighton and Penny, 1969; Reed, 1969). T racheal explants are capable of maintaining the growth of some viruses for up to 6 weeks (Craighead, 1970) and sustained infection of explants may provide a model system for some persistent or latent infections of animals and man which are poorly understood in terms of their pathogenesis and epidemiology. The present work was undertaken to study the growth patterns of o\inr adenovirus type 2 (OA2), at the cellular and sub-cellular levels and some 01 the responses of infected tracheal explants over a period of 6 weeks. MATERIALS
AKD
METHODS
Organ cultures. The procedure was based on that of Tyrrell and Blamire (196!; with trachea obtained from foetal lambs. Tissues were removed aseptically, rinsed in phosphate-buffered saline (PBS) and allowed to soak for 1 h in PBS containing penicillin ( 1000 units per ml), streptomycin (500 pg per ml), kanamycin (200 pg per ml:) and mycostatin (100 pg per ml). The adventitia was then removed, and the rinss cut into squares of 3 to 4 mm which were explanted on their adventitial surface In groups of 4 in 60-mm plastic Petri dishes; each dish had been prepared with scalpclincised square grids (5 mm), to which the explants adhered, and contained 3 ml of’ medium. The standerd solution was Eagle’s minimal essential medium supplemcntccl \vith 0.2 per cent bovine plasma albumin, 0.088 per cent sodium bicarbonate and standard concentrations of antibiotics. Incubation was carried out in a 5 per ct’nt
P.lTrr.
1979.
\.OL.
IXFECTION
lOi
89.
OF TRACHEAL ORGAK OVINE ADESOVIRUS
CULTURES TYPE 2
\YITH
B)
R.
S.
F.
CAMPBELL,*
:lnimn/
A. RAE,
J.
Diseaser Research Association.
Moredun
31.
SHARP Imhte.
and
SMITH
It:.
ICdinbur~qh, Scotland
INTRODUCTION
t:ultivation of viruses in tracheal explants has become a standard research method and various aspects of the growth and pathogenesis of a range of human and animal viruses have been described (Campbell, Thompson, Leighton and Penny, 1969; Reed, 1969). T racheal explants are capable of maintaining the growth of some viruses for up to 6 weeks (Craighead, 1970) and sustained infection of explants may provide a model system for some persistent or latent infections of animals and man which are poorly understood in terms of their pathogenesis and epidemiology. The present work was undertaken to study the growth patterns of o\inr adenovirus type 2 (OA2), at the cellular and sub-cellular levels and some 01 the responses of infected tracheal explants over a period of 6 weeks. MATERIALS
AKD
METHODS
Organ cultures. The procedure was based on that of Tyrrell and Blamire (196!; with trachea obtained from foetal lambs. Tissues were removed aseptically, rinsed in phosphate-buffered saline (PBS) and allowed to soak for 1 h in PBS containing penicillin ( 1000 units per ml), streptomycin (500 pg per ml), kanamycin (200 pg per ml:) and mycostatin (100 pg per ml). The adventitia was then removed, and the rinss cut into squares of 3 to 4 mm which were explanted on their adventitial surface In groups of 4 in 60-mm plastic Petri dishes; each dish had been prepared with scalpclincised square grids (5 mm), to which the explants adhered, and contained 3 ml of’ medium. The standerd solution was Eagle’s minimal essential medium supplemcntccl \vith 0.2 per cent bovine plasma albumin, 0.088 per cent sodium bicarbonate and standard concentrations of antibiotics. Incubation was carried out in a 5 per ct’nt
