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Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 016007 (http://iopscience.iop.org/1752-7163/9/1/016007) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 142.66.3.42 This content was downloaded on 25/06/2016 at 14:19
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J. Breath Res. 9 (2015) 016007
doi:10.1088/1752-7155/9/1/016007
Paper
received
29 October 2014
Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours
re vised
20 January 2015 accep ted for publication
23 January 2015 published
9 March 2015
F W Albrecht1,3, T Hüppe1,3, T Fink1, F Maurer1, A Wolf1, B Wolf1, T Volk1, J I Baumbach2 and S Kreuer1 1
Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center and Saarland University Faculty of Medicine, Building 57, D-66421 Homburg, Germany 2 Faculty of Applied Chemistry, Reutlingen University, Alteburgstrasse 150, D-72762 Reutlingen, Germany E-Mail: [email protected] Keywords: multi-capillary column (MCC), ion mobility spectrometry (IMS), volatile organic compounds (VOCs), rat model, breath analysis
Abstract Long-term animal studies are needed to accomplish measurements of volatile organic compounds (VOCs) for medical diagnostics. In order to analyze the time course of VOCs, it is necessary to ventilate these animals. Therefore, a total of 10 male Sprague–Dawley rats were anaesthetized and ventilated with synthetic air via tracheotomy for 24 h. An ion mobility spectrometry coupled to multi-capillary columns (MCC–IMS) was used to analyze the expired air. To identify background contaminations produced by the respirator itself, six comparative measurements were conducted with ventilators only. Overall, a number of 37 peaks could be detected within the positive mode. According to the ratio peak intensity rat/ peak intensity ventilator blank, 22 peaks with a ratio >1.5 were defined as expired VOCs, 12 peaks with a ratio between 0.5 and 1.5 as unaffected VOCs, and three peaks with a ratio
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About
Contact us
My IOPscience
Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 016007 (http://iopscience.iop.org/1752-7163/9/1/016007) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 142.66.3.42 This content was downloaded on 25/06/2016 at 14:19
Please note that terms and conditions apply.
J. Breath Res. 9 (2015) 016007
doi:10.1088/1752-7155/9/1/016007
Paper
received
29 October 2014
Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours
re vised
20 January 2015 accep ted for publication
23 January 2015 published
9 March 2015
F W Albrecht1,3, T Hüppe1,3, T Fink1, F Maurer1, A Wolf1, B Wolf1, T Volk1, J I Baumbach2 and S Kreuer1 1
Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center and Saarland University Faculty of Medicine, Building 57, D-66421 Homburg, Germany 2 Faculty of Applied Chemistry, Reutlingen University, Alteburgstrasse 150, D-72762 Reutlingen, Germany E-Mail: [email protected] Keywords: multi-capillary column (MCC), ion mobility spectrometry (IMS), volatile organic compounds (VOCs), rat model, breath analysis
Abstract Long-term animal studies are needed to accomplish measurements of volatile organic compounds (VOCs) for medical diagnostics. In order to analyze the time course of VOCs, it is necessary to ventilate these animals. Therefore, a total of 10 male Sprague–Dawley rats were anaesthetized and ventilated with synthetic air via tracheotomy for 24 h. An ion mobility spectrometry coupled to multi-capillary columns (MCC–IMS) was used to analyze the expired air. To identify background contaminations produced by the respirator itself, six comparative measurements were conducted with ventilators only. Overall, a number of 37 peaks could be detected within the positive mode. According to the ratio peak intensity rat/ peak intensity ventilator blank, 22 peaks with a ratio >1.5 were defined as expired VOCs, 12 peaks with a ratio between 0.5 and 1.5 as unaffected VOCs, and three peaks with a ratio
