Int J Clin Exp Pathol 2015;8(9):11340-11347 www.ijcep.com /ISSN:1936-2625/IJCEP0013351
Original Article Inhibition effect of Peg-IFNα-2b and Imatinib alone or combination on imatinib-resistant gastrointestinal stromal tumors cell lines Lei-Yi Zhang, Jiang-Sheng Huang, Zhi-Min Pi, Mei-Ying Yu Department of Minimally Invasive Surgery, The Second Xiangya Hospital of Central South University, Changsha 421001, Hunan, China Received July 24, 2015; Accepted August 26, 2015; Epub September 1, 2015; Published September 15, 2015 Abstract: To investigate the inhibition effect of polyethylene glycol interferon α-2b and imatinib alone or combination on imatinib-resistant GIST cell lines, and to explore the possible mechanism. Imatinib resistant GIST cell lines (GIST-R) were exposured to either Peg-IFNα-2b or imatinib alone or combination. The proliferative inhibition rates and the combination index (CI) values of GIST-R cells were detected by MTT assay. The apoptotic rates of GIST-R cells were detected by flow cytometry. The expression levels of phospho-mammalian target of rapamycin (p-mTOR), and Bcl-2 of GIST-R cells were analyzed by Western blot. GIST-R cells presents remarkable resistance to imatinib, and the resistance index (RI) were (P
Original Article Inhibition effect of Peg-IFNα-2b and Imatinib alone or combination on imatinib-resistant gastrointestinal stromal tumors cell lines Lei-Yi Zhang, Jiang-Sheng Huang, Zhi-Min Pi, Mei-Ying Yu Department of Minimally Invasive Surgery, The Second Xiangya Hospital of Central South University, Changsha 421001, Hunan, China Received July 24, 2015; Accepted August 26, 2015; Epub September 1, 2015; Published September 15, 2015 Abstract: To investigate the inhibition effect of polyethylene glycol interferon α-2b and imatinib alone or combination on imatinib-resistant GIST cell lines, and to explore the possible mechanism. Imatinib resistant GIST cell lines (GIST-R) were exposured to either Peg-IFNα-2b or imatinib alone or combination. The proliferative inhibition rates and the combination index (CI) values of GIST-R cells were detected by MTT assay. The apoptotic rates of GIST-R cells were detected by flow cytometry. The expression levels of phospho-mammalian target of rapamycin (p-mTOR), and Bcl-2 of GIST-R cells were analyzed by Western blot. GIST-R cells presents remarkable resistance to imatinib, and the resistance index (RI) were (P
