www.nature.com/scientificreports
OPEN
received: 31 March 2016 accepted: 24 August 2016 Published: 15 September 2016
Investigating the Responses of Human Epithelial Cells to Predatory Bacteria Ajay K. Monnappa*, Wasimul Bari*, Seong Yeol Choi & Robert J. Mitchell One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrioand-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E. coli. This was true even though the latter strain was added at an 11.1-fold lower concentration (p
OPEN
received: 31 March 2016 accepted: 24 August 2016 Published: 15 September 2016
Investigating the Responses of Human Epithelial Cells to Predatory Bacteria Ajay K. Monnappa*, Wasimul Bari*, Seong Yeol Choi & Robert J. Mitchell One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrioand-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E. coli. This was true even though the latter strain was added at an 11.1-fold lower concentration (p
