Journal oflmmunological Methods, 27 (1979) 273--282
273
© Elsevier/North-Holland Biomedical Press
IS THE d AN ALLELIC GENE OF D IN THE Rh BLOOD GROUP SYSTEM? A QUANTITATIVE STUDY USING 99mTECHNETIUM-PYROPHOSPHATE-LABELLED ANTIBODY (TcLA) METHOD *
V.A. TOMA **, M. I T U R R A L D E , J.D. ANDERSON and F.P. R E T I E F
Departments of Haematology, Nuclear Medicine and Oncotherapy, Faculty of Medicine, University of the Orange Free State, 9300 Bloemfontein, Republic of South Africa (Received 22 November 1978, accepted 3 December 1978)
This article reports on studies of Rh antigens, such as D, D u and d, using uranyllabelled antibody (ULA) and TcLA (99mTc-pyrophosphate-labelled antibody) methods for the first time for this purpose. TcLA method proved to be simple in labelling and very sensitive (20--100 times more so than the indirect Coombs test) in the detection of Rh antigen-antibody binding. Results of this quantitative study demonstrate convincingly that the d is not an allelic gene of D but rather the weakest of the series D
273
© Elsevier/North-Holland Biomedical Press
IS THE d AN ALLELIC GENE OF D IN THE Rh BLOOD GROUP SYSTEM? A QUANTITATIVE STUDY USING 99mTECHNETIUM-PYROPHOSPHATE-LABELLED ANTIBODY (TcLA) METHOD *
V.A. TOMA **, M. I T U R R A L D E , J.D. ANDERSON and F.P. R E T I E F
Departments of Haematology, Nuclear Medicine and Oncotherapy, Faculty of Medicine, University of the Orange Free State, 9300 Bloemfontein, Republic of South Africa (Received 22 November 1978, accepted 3 December 1978)
This article reports on studies of Rh antigens, such as D, D u and d, using uranyllabelled antibody (ULA) and TcLA (99mTc-pyrophosphate-labelled antibody) methods for the first time for this purpose. TcLA method proved to be simple in labelling and very sensitive (20--100 times more so than the indirect Coombs test) in the detection of Rh antigen-antibody binding. Results of this quantitative study demonstrate convincingly that the d is not an allelic gene of D but rather the weakest of the series D
