Gene. 9X (1991)

149-150

149

Elsevier

GENE

03935

Corrigendum Gene, 90 (1990) 93-9X Elsevier

GENE

03565

Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Sacchammyces cerevisiae (Recombinant

DNA;

yeast; cell-division

cycle; bud emergence;

helix-turn-helix;

DNA-binding

proteins)

Douglas 1. Johnson n-b, Jeanne M. O’Brien” and Charles W. Jacobsb ” Department of Microbio1og.v and Molecular Genetics, University of Vermont, Burlington, VT05405 University of Michigan, Ann Arbor, MI 48109 (U.S.A.) Received by J. Marmur: Accepted: 15 November

27 October 1990

(U.S.A.)

and h Department of Biology, The

1990

We have discovered errors in our published sequence of the Succhuromvce.s cerevisiae CDC43 gene (Johnson et al., 1990). The correct nucleotide (nt) and amino acid (aa) sequences are shown in Fig. 1 below. The CDC43 gene is identical in nt and aa sequence to the S. cerevisiue CAL1 gene (Ohya et al., 1991), which is very similar in deduced aa sequence to the S. cerevisiue DPRl gene product (Goodman et al., 1988), a protein involved in the C-terminal modification of RAS proteins in S. cerevisiae. The synthetic lethality of a c&42 cdc43 double mutant (Adams et al., 1990) can now be rationalized if the CDC43 gene product is involved in the post-translational modification of the CDC42 gene product. Interestingly, the terminal morphologies of a CDC43 temperature-sensitive mutant (Adams et al., 1990) and a Ca2 + -dependent CAL1 mutant (Ohya et al., 1984) grown under restrictive conditions are not identical; a CDC43 mutant arrests as large, unbudded cells and a CAL1 mutant arrests as cells with small buds. Clarification of this discrepancy must await molecular analysis of the nature of the conditional mutations.

ACKNOWLEDGEMENTS

We thank M. Goebl (Indiana University School of Medicine). F. Tamanoi (University of Chicago) and Y. Ohya and Y. Anraku (University of Tokyo) for unpublished data which led to our discovery of the sequencing errors.

REFERENCES Adams,

A.E.M.,

Johnson.

D.I.,

Longnecker,

R.M.,

Pringle, J.R.: CDC42 and CDC43, two additional budding

and the establishment

of cell polarity

Molecular Burlington,

Department

of Microbiology

and

Genetics, University of Vermont, A-191 Given Building, VT 05405 (U.S.A.) Tel. (802)656-8203; Fax (802)656-85X4.

0378-l I IY:Y1:$03.50 0 1991 Elscvicr Scrence Publishers B.V (Biomedical Division)

B.F. and in

in the yeast Saccharo-

maces cerevisiae. J. Cell. Biol. 111 (1990) 131-143. Goodman, L.E., Perou, CM., Fujiyama, A. and Tamanoi, F.: Structure and expression ofyeast DPRI, a gene essential for the processing and intracellular Johnson,

localization

of ras proteins.

D.I., O’Brien, J.M. and Jacobs,

analysis

of CDC43, a gene involved

Yeast 4 (1988) 271-281.

C.W.: Isolation in the control

and sequence

of cell polarity

in

Saccharomyces cerevisiae. Gene 90 (I 990) Y3-98. Ohya, Y., Ohsumi,

Y. and Anraku,

Y.: Genetic

study ofthe role ofcalcium

ions in the cell division cycle of Saccharomyces cerevisiue:a calciumdependent

mutant

and

its

trifluoperazine-dependent

revertants. Mol. Gen. Genet. 193 (1984) 3X9-394. Ohya, Y., Goebl, M., Goodman, L.E., Petersen-Bjorn,

Correspondence to: Dr. D.I. Johnson,

Sloat,

genes involved

Tamanoi,

F. and Anraku,

tional homolog

pseudo-

S., Friesen,

Y.: Yeast CAL1 is a structural

J.D.,

and func-

to the DPRI (RAM) gene involved in RAS-processing.

J. Biol. Chem. (1991) in press.

150 GA TCTTCTTTTT TACCTGTTTT ATTCACTTTT TTTTTTTTTT CTGAGTTGTT GCGCCTTGGA AGAATGGAAA AGCAATAGTT TCAGTGACTA TAGTATACAA TCAAAACACC CGCCAGTTCA AAACTATTAC TTGGTCACTA ACCCCCAAGT CATCTCTAGC AATTTAAATT TCAATTTTAu4 GAAAACCTTT ATTTATCCAA CGTGAACMG TACTTTCAAG CACTTCTGCC CACCGCTATA TCGTGGAAA.4

-211 -141 -71 -1

ATG TGT C,AAGCT ACC AAT GGC CCG AGT AGA CTT GTG ACT AAA AAG CAT AGG AA.4TTT TTC GM Met Cys Gin Ala Thr Asn Gly Pro Ser Arg Val Val Thr Lys Lys His Arg LjfsPhe Phe Glu

63

AGA CAT CTA CAG TTG CTT CCC TCT TCA CAT GAG GGA CAT GAC GTG MC AGA ATG GCG AT.4ATA Arg His Leu Gin Leu Leu Pro Ser Ser His Gin Gly His Asp Val Asn Arg Met Ala Ile ILe

1?6

TTC TAC TCA ATC TCA GGA CTC TCT ATA TTT GAT GTT AAC GTT TCT GCG AAG TAG GGC GAT CAT Phe Tyr Ser Ile Ser Gly Leu Ser Ile Phe Asp Val As,,Val SPY Ala Lys Tyr Gly Asp His

189

CTT GGC TGG ATG CGC AAA CAT TAT ATC AAA ACA GTG CTG GAT GAT ACA GAA AAT ACT GTG ATA Leu Gly Trp Met Arg Lys His Tyr Ile Lys Thr Val Leu Asp Asp Thr Glu Asn Thr Val Ile

li?

TCT GGA TTT GTT GGA AGC TTA GTC ATG AAT ATC CCT CAC GCA ACA ACG ATT MT CTA CCA A.4T Iii Ser Gly Phe Val GLy Ser Leu Val Met Asn ILe Pro His Ala Thr Thr Ile Asn Leu Pro AS~ ACT CTC TTT GCA TTG TTG TCC ATG ATT ATG CTG AGA GAT TAC GAG TAT TTT GAG ACT ATA CTA Thr Leu Phe Ala Leu Leu Ser Met Ile ?letLeu Arg Asp Tyr Glu Tyr Phe Glu Thr Ile Leu

378

GAC AAA AGA AGC CTG GCG AGA TTT GTT TCT AAG TGC CAA CGA CCT GAC CGT GGC TCG TTT GTA Asp Lys Arg Ser Leu Ala Arg Phe Val Ser Lys Cys Gin Arg Pro Asp Arg Gly Ser Phe "al

!,!+L

TCT TGT TTA GAC TAT AAG ACA AAT TCT GGA TCT TCG GTT CAT TCA GAC GAT TTA AGG TTT TGC Ser Cys Leu Asp Tyr Lys Thr Asn Cys Gly Ser Ser Val Asp Ser Asp Asp Leu Arg Phe Cys

5Oi

TAC ATC GCA GTT GCC ATT CTG TAC ATA TGC GGA TGC CGA TCC AA4 GAA GAC TTT GAT GAA TAC Tyr Ile Ala Val Ala Ile Leu Tyr Ite Cys Gly Cys Arg Ser Lvs GLu Asp Phe Asp Glu Tyr

',h?

ATT GAT ACT GAG AAG TTG CTT GGC TAT ATA ATG TCG CM CAA TGC TAG AAC CGA GCT TTC CGT Ile Asp Thr Glu Lys Leu Leu Gly Tyr Ile Met Ser Gin Gin Cvs Tyr Asn Gly Ala Phe Gly

630

GCC CAC AAT G.AACCA CAC TCA GGC TAC ACA TCT TGT CCG CTG TCT ACC TTA GCT TTA CTC TCT Ala His Asn Glu Pro His Ser Glv Tyr Thr Ser Cys Ala Leu Ser Thr Leu Ala Leu Leu Ser

hl:

AGT TTG GAA AAG CTA TCA GAG MG TTT .@A GAA GAC ACC ATA ACC TGG CTA TTA CAT AGG CA.4 : I'> Ser Leu Glu Lys Leu Ser Asp Lys Phc Lys Glu Asp Thr Ile Thr Trp Leu Leu His 4rg 61~1 GTA TCA AGC CAT GGA TGT ATG AAA TTT GM AGC GM TTG MT GCC AGC TAT GAT CM TCT GAT Val Ser Sex His Gly Cys Net Lys Phe Glu Ser Glu Leu Asn ALa Ser Tyr Asp Gin Ser Asp

ii'

CAT GGC GGT TTC CAG GGA AGG GAG AAC A_4GTTC GCT CAT ACG TGT TAC GCA TTT TGG TGC TT.4 ?O.' Asp Gly Gly Phe Gin Gly Arg Giu Asn Lys Phe Ala Asp Thr Cys Tyr Ala Phe Trp C;s Leu MT As,,

,,t,

TCA CTA CAC TTA CTA ACA AAG GAT TGG AAA ATC CTA TGC CAA ACT GM CT.4GTC ACA AAT Ser Leu His Leu Leu Thr Ljs Asp lrp Lys Het Leu Cys Gin Thr Glu Leu S'alThr Asn

TAT TTG CTT GAT CGA ACG Cti .kU 412 TTA ACT GCA GGC ITT AGT AAA AAT GAC G?A GW GAT 11"3 T;.rLeu Leu Asp Arg Thr Gin Lvs Thr Leu Thr Glv Gl:iPhe Ser Lys Asn Asp Glu Glu Asp GCT CAT TTA TAT CAC AGC TCT CTA :;GGXC GCT GCG TTA GCA TTA ATT GAG GGG .AAATTT AAT 1'1'1 Ala Asp Leu Tyr His Ser Cys Leu Gly Ser Ala Ala Leu Ala Leu Ile Glu Gly Lys Phe hsn GCA GAA TTA TCC ATA CCT CAA GA,?ATA TTT .UT CAT TTT AGT .AAAAGG TGC TCT TTT TGA Gly Glu Leu Cys Ile Pro Gin Glu Ile Phe Asn Asp Phe Ser Lvs Arg Cvs Cys Ph?

Fig. 1. Nucleotide

sequence

of the CDC43 gene and predicted

of 376 aa, which is 163 aa longer than our published codon. The GenBank adding

accession

number

version

aa sequence (Johnson

is M31114. The corrected

an ‘A’ at nt 724 and 191 additional

of the CDC43 product.

The CDC43 gene is predicted

et al., 1990). The nt sequence

nt sequence

nt at the end of the published

was generated

sequence.

11!1

is numbered

by removing

The remainder

relative

to encode a polypeptidc

to the A of the putative

star

a ‘c’ at nt 637 and a ‘T’ at nt 691, and b:

of the published

sequence

is unchanged.