Journal of Visualized Experiments

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Materials List for:

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells 1

Yan Xing , Kristin A. Hogquist

1

1

Department of Laboratory Medicine & Pathology, Center for Immunology, University of Minnesota

Correspondence to: Yan Xing at [email protected] URL: http://www.jove.com/video/51780 DOI: doi:10.3791/51780

Materials Name

Company

Catalog Number

Comments

Anti-mouse EpCAM

eBioscience

XX-5791-YY*

Clone G8.8

Anti-mouse MHC II

eBioscience

XX-5321-YY*

Clone M5/114.15.2

Anti-mouse Ly51

eBioscience

XX-5891-YY*

Clone 6C3

UEA-1

Vector Laboratory

FL-1061

Flow cytometer

BD Biosciences

LSRFortessa

Flow cell sorter

BD Biosciences

FACSAria

FACS tubes

BD Biosciences

352052

Flow cytometry analysis software

TreeStar - Flowjo

FlowJo v7/9

*XX varies by fluorochrome and YY varies by vial size.

Copyright © 2014 Journal of Visualized Experiments

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Isolation, identification, and purification of murine thymic epithelial cells.

The thymus is a vital organ for T lymphocyte development. Of thymic stromal cells, thymic epithelial cells (TECs) are particularly crucial at multiple...
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