Journal of Visualized Experiments
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Materials List for:
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells 1
Yan Xing , Kristin A. Hogquist
1
1
Department of Laboratory Medicine & Pathology, Center for Immunology, University of Minnesota
Correspondence to: Yan Xing at
[email protected] URL: http://www.jove.com/video/51780 DOI: doi:10.3791/51780
Materials Name
Company
Catalog Number
Comments
Anti-mouse EpCAM
eBioscience
XX-5791-YY*
Clone G8.8
Anti-mouse MHC II
eBioscience
XX-5321-YY*
Clone M5/114.15.2
Anti-mouse Ly51
eBioscience
XX-5891-YY*
Clone 6C3
UEA-1
Vector Laboratory
FL-1061
Flow cytometer
BD Biosciences
LSRFortessa
Flow cell sorter
BD Biosciences
FACSAria
FACS tubes
BD Biosciences
352052
Flow cytometry analysis software
TreeStar - Flowjo
FlowJo v7/9
*XX varies by fluorochrome and YY varies by vial size.
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