Nucleic Acids Research, Vol. 18, No. 22 6689
.=) 1990 Oxford University Press
Isolation of protein s3
a
cDNA encoding human 40S ribosomal
X.T.Zhang, Y.-M.Tan and Y.H.Tan* Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511 Singapore Submitted October 16, 1990
EMBL accession
A cDNA which encodes human 40S ribosomal protein s3 has been isolated from a lambda-Gem2 cDNA library constructed using poly (A+) RNA from human WISH cells. The cDNA insert includes a 5' noncoding sequence of 22 nucleotides, an open reading frame of 729 nucleotides and a 3' noncoding sequence of 82 nucleotides followed by a 3' poly A tail. The hexameter AATAAA, which is the signal sequence for polyadenylation of the 3' end of pre-mRNA (1), is located at position 812-817 in the 3' noncoding region. The open reading frame encodes a putative protein of 243 amino acid residues. The initiation codon occurs in the context AAGATGG, which conforms to the optimal translation start consensus sequence (2). The polypeptide deduced from the cDNA sequence shows 98 % homology with the rat ribosomal protein s3 (accession number
REFERENCES 1. Proudfoot,N.J. and Brownlee,G.G. (1976) Nature 263, 211-214. 2. Kozak,M. (1986) Cell 44, 283-292. 3. Amaldi,F., Beccari,E., Bozzoni,I., Luo,Z. and Pierandrei-Amaldi,P. (1982) Gene 17, 311-316. 4. Smith,T.F. and Waterman,M.S. (1981) J. Mol. Biol. 147, 195-197.
60
TGATGGCATCTTCAAAGCTGAACTGAATGAGTTTCTTACTCGGGAGCTGGCTGAAGATGG d g i f k a e 1 n e f 1 t r e 1 a e d g
120
s
g
v
e
v
r
v
t
p
t
r
to
180
iii
1
a
t
CAGAACACAGAATGTTCTTGGTGAGAAGGGCCGGCGGATTCGGGAACTGACTGCTGTAGTI r t q n v 1 g e k g r r ir e 1 t a v v
240
TCAGAAGAGGTTTGGCTTTCCAGAGGGCAGTGTAGAGCTTTATGCTGAAAAGGTGGCCAC
300
q
k
r
f
g
f
p
e
g
s
v
e
1
y
a
e
k
v
a
t
TAGAGGTCTGTGTGCCATTGCCCAGGCAGAGTGTCTGCGTTACAAACTCCTAGGAGGGCT r g 1 c a i a q a e c 1 r y k 1 1 g g 1
360
TGCTGTGCGGAGGGCCTGCTATGGTGTGCTGCGGTTCATCATGGAGAGTGGGGCCAAAGG
420
a
v
r
r
a
c
y
g
v
1
r
f
i
m
e
s
g
a
k
g
CTGCGAGGTTGTGGTGTCTGGGAAACTCCGAGGACAGAGGGCTAAATCCATGAAGTTTGT c
e
v
v
v
s
g
k
1
r
g
q
r
a
k
s
m
k
f
v
d
g
1
m
i
h
a
g
d
p
v
n
y
y
v
d
t
a
v
r
480 540
CCACGTGTTGCTCAGACAGGGTGTGCTGGGcATCAAGGTGAAGATCATGCTGCCCTGGGA h v 1 1 r q g v 1 g i k v k i m 1 p w d
600
CCCAACTGGTAAGATTGGCCCTAAGAAGCccCTGCCTGACCACGTGAGCATTGTGGAACC p t g k i g p k k p 1 p d h v s i v e p
660
TAAAGATGAGATACTGCCCACCACCCCCATCTCAGAACAGAAGGGTGGGAAGCCAGAGCT k d e i 1 p t t p i e q k g g k p e 1
720
GCCTGCCATGCCCCAGCCAGTCCCCACAGCATAACAGGGTCTCCTTGGCAGCTGCATTCT p a m p q p v p t a
780 833
*
To whom correspondence should be addressed
X55715
P17073). Amino acid residues from position 146 to 216 of the human 40S ribosomal protein s3 exhibit 90% identity with 40S ribosomal protein sl of Xenopus laevis (3), compared using the Smith and Waterman method (4). The size of the mRNA of ribosomal protein s3 is about 900 bases as revealed by Northern blot.
CGTTGCTGTCGGCGGCGGCAAGATGGCAGTGCAAATATCCAAGAAGAGGAAGTTTGTCGC m a v q i s k k r k f v a
y
no.
.=) 1990 Oxford University Press
Isolation of protein s3
a
cDNA encoding human 40S ribosomal
X.T.Zhang, Y.-M.Tan and Y.H.Tan* Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511 Singapore Submitted October 16, 1990
EMBL accession
A cDNA which encodes human 40S ribosomal protein s3 has been isolated from a lambda-Gem2 cDNA library constructed using poly (A+) RNA from human WISH cells. The cDNA insert includes a 5' noncoding sequence of 22 nucleotides, an open reading frame of 729 nucleotides and a 3' noncoding sequence of 82 nucleotides followed by a 3' poly A tail. The hexameter AATAAA, which is the signal sequence for polyadenylation of the 3' end of pre-mRNA (1), is located at position 812-817 in the 3' noncoding region. The open reading frame encodes a putative protein of 243 amino acid residues. The initiation codon occurs in the context AAGATGG, which conforms to the optimal translation start consensus sequence (2). The polypeptide deduced from the cDNA sequence shows 98 % homology with the rat ribosomal protein s3 (accession number
REFERENCES 1. Proudfoot,N.J. and Brownlee,G.G. (1976) Nature 263, 211-214. 2. Kozak,M. (1986) Cell 44, 283-292. 3. Amaldi,F., Beccari,E., Bozzoni,I., Luo,Z. and Pierandrei-Amaldi,P. (1982) Gene 17, 311-316. 4. Smith,T.F. and Waterman,M.S. (1981) J. Mol. Biol. 147, 195-197.
60
TGATGGCATCTTCAAAGCTGAACTGAATGAGTTTCTTACTCGGGAGCTGGCTGAAGATGG d g i f k a e 1 n e f 1 t r e 1 a e d g
120
s
g
v
e
v
r
v
t
p
t
r
to
180
iii
1
a
t
CAGAACACAGAATGTTCTTGGTGAGAAGGGCCGGCGGATTCGGGAACTGACTGCTGTAGTI r t q n v 1 g e k g r r ir e 1 t a v v
240
TCAGAAGAGGTTTGGCTTTCCAGAGGGCAGTGTAGAGCTTTATGCTGAAAAGGTGGCCAC
300
q
k
r
f
g
f
p
e
g
s
v
e
1
y
a
e
k
v
a
t
TAGAGGTCTGTGTGCCATTGCCCAGGCAGAGTGTCTGCGTTACAAACTCCTAGGAGGGCT r g 1 c a i a q a e c 1 r y k 1 1 g g 1
360
TGCTGTGCGGAGGGCCTGCTATGGTGTGCTGCGGTTCATCATGGAGAGTGGGGCCAAAGG
420
a
v
r
r
a
c
y
g
v
1
r
f
i
m
e
s
g
a
k
g
CTGCGAGGTTGTGGTGTCTGGGAAACTCCGAGGACAGAGGGCTAAATCCATGAAGTTTGT c
e
v
v
v
s
g
k
1
r
g
q
r
a
k
s
m
k
f
v
d
g
1
m
i
h
a
g
d
p
v
n
y
y
v
d
t
a
v
r
480 540
CCACGTGTTGCTCAGACAGGGTGTGCTGGGcATCAAGGTGAAGATCATGCTGCCCTGGGA h v 1 1 r q g v 1 g i k v k i m 1 p w d
600
CCCAACTGGTAAGATTGGCCCTAAGAAGCccCTGCCTGACCACGTGAGCATTGTGGAACC p t g k i g p k k p 1 p d h v s i v e p
660
TAAAGATGAGATACTGCCCACCACCCCCATCTCAGAACAGAAGGGTGGGAAGCCAGAGCT k d e i 1 p t t p i e q k g g k p e 1
720
GCCTGCCATGCCCCAGCCAGTCCCCACAGCATAACAGGGTCTCCTTGGCAGCTGCATTCT p a m p q p v p t a
780 833
*
To whom correspondence should be addressed
X55715
P17073). Amino acid residues from position 146 to 216 of the human 40S ribosomal protein s3 exhibit 90% identity with 40S ribosomal protein sl of Xenopus laevis (3), compared using the Smith and Waterman method (4). The size of the mRNA of ribosomal protein s3 is about 900 bases as revealed by Northern blot.
CGTTGCTGTCGGCGGCGGCAAGATGGCAGTGCAAATATCCAAGAAGAGGAAGTTTGTCGC m a v q i s k k r k f v a
y
no.
