Veterinary hnmunology and Immunopathology, 27
( 1991 ) 8-11
Elsevier Science Publishers B.V., Amsterdam
1.3 Monoclonal antibodies submitted for inclusion in the panel
Table 1 lists the mAbs submitted by the laboratories that were assembled to form the panel of reagents examined. MAbs are listed by the individual code numbers given them by their suppliers under a sub-heading which is the laborator3, of origin. These codes for the individual mAbs are used throughout this publication. In some cases descriptions of the mAbs have been published and the antigens recognised defined. The original references for individual mAbs are also given in an abbreviated form in Table 1. TABLE 1
Monoclonal antibodies submitted for inclusion in the panel MAb code (other name ) ILRAD IL-AI !' IL-AI2 I IL-AI 5 IL-AI6 IL-A222
Specificity
MAb code (other name )
Specificity
Madison BoT4, CD4 BoT4, CD4
Pullman BoT2, CD2
T-mature, CD6 T-mature, CD6
IL-A55 IL-A57 IL-A65 IL-A67 IL-A87 (J4)
0165-2427/91/$03.50
TH97A a CH128A 6 CHI32A BAQ95A CACT31A BAT 18A BAT42A BAT76A BAQ82A BAQ91A CACTI41A CACT83B CACT87A CACT 138A GCIAI GCSOAI BAQI 11A CACT80C CACT88C CACT 130A
Specificity
Melbourne
C5B6 8-3F4 16-1El0 8-10C5 8-1E3
II A "t'~2 | 1 --rt L,.3
IL-A242 IL-A25 IL-A263 IL-A273 IL-A283 IL-A29 IL-A42 IL-A43 IL-A45 IL-A46 IL-A47 IL-ASI IL-A53 lL-A54
MAb code (other name )
BoTl, CDI BoT2~CD2
CBU-72-87 SBU-25-32 ~ CBU-TI SBU-38-65 (T8) a SBU-20-27 (T6) 9 SBU-19-19 (TI9) !° SBU-l-28 (LCA) ~1 CBU-72-37 SBU-25-91(Tl)9 SBU-44-38 (T4) s SBU-44-97 (T4)S SBU-38-3812 SBU-20-9613 SBU-46-6612 SBU-38-51 t4
Edinburgh VPM30 VPM3GB (clone 6.1 ) VPM31 VPM32 VPM33 VPM5 VPM 18 VPM29 VPM29B (clone 2. l )
© 1991 - - Elsevier Science Publishers B.V.
CD l I a CD44 CD5 CD8 CDI CD45 CD45R CD5 CD4 CD4 CD45R
TABLE 1 (continued) MAb code (other name) IAH Compton CC8 CC 134 CC144 CC15 CC175 CC20 CC21 CC28 CC295 CC30 CC37 CC38 CC39 CC42 CC43 CC49 CC51 CC55 CC56 CC57 CC58 CC60 CC63
Specificity
BoTI, CDI BoT 1, CD 1 Bo5, CD5
BoS, CD5
MAb code (other name) BAT82A BAS9A BAQ44A BAQ 129A BAQI55A CH 127A B7A 1 BAQ4A BAQ53A BAQ89A BAQ90A BAQ 128A BAQI36A BAQ159A CACTB6A CACTB31A CACTB32A CACTB35A CACTB81A CH 16A2 CHI37A GS23A CHI38A
Specificity
MAb code (other name)
Specificity
Storrs BLT- 125 Basel ST-IA 16 T8027 ST.4a ~s ST-829 ST-I 128 135-A2° 19722
CD5 CD4 CD8 CD2 CD2
181
B5-522 B 18 151 S-MHCII TIITS 23 175 FI50 SB-I Namur 8CII 24 NAM 1 ( 17A3 ) NAM2 (11F5) NAM3 (1H8) NAM4 (2G 1)
CD59 CD45 (LCA) MHCII LFA-3, CD58 CDI la, LFA-I MHC II CD5
Kitasato BLMo-425 BLM0-1025 ~Baldwin et al., 1986. 2Ellis et al., 1988. 3Baldwin et al., 1988.4MacHugh et al., 1988.5Howard et al., 1988. 6Davis et al., 1988. 1Mackay et al., 1988b. aMaddox et al., 1985b. 9Mackay et al., 1985. '°Mackay et al., 1986. ~2Maddox et al., 1985a. 22Maddox et al., 1987. 23Mackay et al., 1987. 24Mackay et al., 1988c. ~SRabinovsky and Yang, 1986. 26Beya et al., 1986. mTMiyasaka et al., 1983. maMackay et al., 1988a. 29Ezaki et al., 1987.2°Giegerick et al., 1989. 2~McClure et al., 1989. 22Hein et al., 1988.23Hunig, 1985.24Depelchin et al., 1989. 25Kunita et al., 1988.
REFERENCES Baldwin, C.L., Teale, A.J., Naessens, J.G., Goddeeris, B.M., MacHugh, N.D. and Morrison, W.I., 1986. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and mufine L3T4. J. Immunol., 136: 4385-4391. Baldwin, C.L., MacHugh, N.D., Ellis, J.A., Naessens, J., Newson, J. and Morrison, W.I., 1988. Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens. Immunology, 63: 439-446.
10 Beya, M.F., Miyasaka, M., Dudler, L., Ezaki, T. and Trnka, Z., 1986. Studies on the differentiation of T lymphocytes in sheep. II. Two monoclonal antibodies that recognize all ovine T lymphocytes. Immunology, 57:115-121. Davis, W.C., Ellis, J.A., MacHugh, N.D. and Baldwin, C.L., 1988. Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2. Immunology, 63:165-167. Depelchin, A., Letesson, J.-J., Lostrie, N., Mammerikx, M., Portetelle, D. and Burny, A., 1989. Bovine leukaemia virus (BLV) infected B-cells express a marker similar to the CD5 T-cell marker. Immunol. Letters, 20: 69-76. Ellis, J.A., Baldwin, C.L., MacHugh, N.D., Bensaid, A., Teale, A.J., Goddeeris, B.M. and Morrison, W.I., 1986. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology, 58:351-358. Ellis, J.A., Davis, W.C., MacHugh, N.D., Emery, D.L., Kaushal, A. and Morrison, W.I., 1988. Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies. Vet. Immunol. lmmunopathol., 19: 325-340. Ezaki, T., Miyasaka, M., Beya, M.-F., Dudler, L. and Trnka, Z., 1987. A murine anti-sheep T8 monoclonal antibody, ST-~, ~hat defines the cytoto~,ic T 'yn:phocyte population, int. ~,rch. All. Appl. Immunol., 82:168-177. Giegerich, G.W., Hein, W.R., Miyasaka, M., Tiefenthaler, G. and Hunig, T., 1989. Restricted expression of CD2 among subsets of sheep thymocytes and T lymphocytes. Immunology, 66: 354-361. Hein, W.R., McClure, S., Beya, M.-F., Dudler, L. and Trnka, Z., 1988. A novel glycoprotein expressed on sheep T and B lymphocytes is involved in a T cell activation pathway. J. Immunol., 140: 2869-2875. Howard, C.J., Parsons, K.R., Jones, B.V., Sopp, P. and Pocock, D.H., 1988. Two monoclonal antibodies (CCI 7, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol., 19:127-139. Hunig, T., 1985. The cell surface molecule recognized by the erythrocyte receptor ofT lymphocytes: Identification and partial characterization using a monoclonal antibody. J. Exp. Med., 162: 890-901. Kunita, S., Koyama, H. and Saito, H., 1988. Preparation and characterization of monoc!ona! antibodies against bovine B lymphocyte surface antigens. Vet. Immunol. Immunopathol., 18: 201-212. MacHugh, N.D., Bensaid, A., Davis. W.C., Howard, C.J., Parsons, K.R., Jones, B. and Kaushal, A., 1988. Characterization of a bovine thymic differentiation antigen analogous to CDI in the human. Scand. J. Immunol., 27: 541-547. Mackay, C., 1988. Sheep leukocyte molecules: a review of their distribution, structure and possible function. Vet. Immunol. Immunopathol., 19: 1-20. Mackay, C.R., Maddox, J.F., Gogolin-Ewens, K.J. and Brandon, M.R., 1985. Characterization of two sheep lymphocyte differentiation antigens, SBU-TI and SBU-T6. Immunology, 55: 729-737. Mackay, C.R., Maddox, J.F., Brandon, M.R., 1986. Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol., 16:19-25. Mackay, C.R., Maddox, J.F., Brandon, M.R., 1987. A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol., 110: 46-55. Mackay, C.R., Hein, W.R., Brown, M.H. and Matzinger, P., 1988. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol., 18:1681-1688. MacKay, C.R., Maddox, J.F., Wijffels, G.L., Mackay, I.R. and Walker, I.D., 1988. Characterisation of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44. Immunology, 65: 93-99.
II McClure, S.J., Hein, W.R., Yamaguchi, K., Dudler, L., Beya, M.-F. and Miyasaka, M., 1989. Ontogeny, morphology and tissue distribution of a unique subset of CD4- CD8- sheep T lymphocytes. Immunol. Cell Biol., 67:215-221. Maddox, J.F., Mackay, C.R. and Brandon, M.R., 1985. The sheep analogue of leucocyte common antigen (LCA). Immunology, 55: 347-353. Maddox, J.F., Mackay, C.R. and Brandon, M.R., 1985b. Surface antigens, SBU-T4 and SBUTS, of sheep T lymphocyte subsets defined by monoclonal antibodies. Immunology, 55: 739748. Maddox, J.F., Mackay, C.R. and Brandon, M.R.. 1987. Ontogeny of ovine lymphocytes. I. An immunohistologic~l study on the development of T lymphocytes in the sheep embryo and fetal thymus. Immunology, 62: 97-105. Miyasaka, M., Heron, I., Dudler, L., Cahill, R.N.P., Forni, L., Knaak, T. and Trnka, Z., 1983. Studies on the differentiation of T lymphocytes in sheep. I. Recognition of a sheep T-lymphocyte differentiation antigen by a monoclonal antibody T-80. Immunology, 49: 545-553. Rabinovsky, E.D. and Yang, T.-J., 1986. Production and characterization of a bovine T cellspecific monoclonal antibody identifying a mature differentiation antigen. J. Immunol., 136: 609-615.
( 1991 ) 8-11
Elsevier Science Publishers B.V., Amsterdam
1.3 Monoclonal antibodies submitted for inclusion in the panel
Table 1 lists the mAbs submitted by the laboratories that were assembled to form the panel of reagents examined. MAbs are listed by the individual code numbers given them by their suppliers under a sub-heading which is the laborator3, of origin. These codes for the individual mAbs are used throughout this publication. In some cases descriptions of the mAbs have been published and the antigens recognised defined. The original references for individual mAbs are also given in an abbreviated form in Table 1. TABLE 1
Monoclonal antibodies submitted for inclusion in the panel MAb code (other name ) ILRAD IL-AI !' IL-AI2 I IL-AI 5 IL-AI6 IL-A222
Specificity
MAb code (other name )
Specificity
Madison BoT4, CD4 BoT4, CD4
Pullman BoT2, CD2
T-mature, CD6 T-mature, CD6
IL-A55 IL-A57 IL-A65 IL-A67 IL-A87 (J4)
0165-2427/91/$03.50
TH97A a CH128A 6 CHI32A BAQ95A CACT31A BAT 18A BAT42A BAT76A BAQ82A BAQ91A CACTI41A CACT83B CACT87A CACT 138A GCIAI GCSOAI BAQI 11A CACT80C CACT88C CACT 130A
Specificity
Melbourne
C5B6 8-3F4 16-1El0 8-10C5 8-1E3
II A "t'~2 | 1 --rt L,.3
IL-A242 IL-A25 IL-A263 IL-A273 IL-A283 IL-A29 IL-A42 IL-A43 IL-A45 IL-A46 IL-A47 IL-ASI IL-A53 lL-A54
MAb code (other name )
BoTl, CDI BoT2~CD2
CBU-72-87 SBU-25-32 ~ CBU-TI SBU-38-65 (T8) a SBU-20-27 (T6) 9 SBU-19-19 (TI9) !° SBU-l-28 (LCA) ~1 CBU-72-37 SBU-25-91(Tl)9 SBU-44-38 (T4) s SBU-44-97 (T4)S SBU-38-3812 SBU-20-9613 SBU-46-6612 SBU-38-51 t4
Edinburgh VPM30 VPM3GB (clone 6.1 ) VPM31 VPM32 VPM33 VPM5 VPM 18 VPM29 VPM29B (clone 2. l )
© 1991 - - Elsevier Science Publishers B.V.
CD l I a CD44 CD5 CD8 CDI CD45 CD45R CD5 CD4 CD4 CD45R
TABLE 1 (continued) MAb code (other name) IAH Compton CC8 CC 134 CC144 CC15 CC175 CC20 CC21 CC28 CC295 CC30 CC37 CC38 CC39 CC42 CC43 CC49 CC51 CC55 CC56 CC57 CC58 CC60 CC63
Specificity
BoTI, CDI BoT 1, CD 1 Bo5, CD5
BoS, CD5
MAb code (other name) BAT82A BAS9A BAQ44A BAQ 129A BAQI55A CH 127A B7A 1 BAQ4A BAQ53A BAQ89A BAQ90A BAQ 128A BAQI36A BAQ159A CACTB6A CACTB31A CACTB32A CACTB35A CACTB81A CH 16A2 CHI37A GS23A CHI38A
Specificity
MAb code (other name)
Specificity
Storrs BLT- 125 Basel ST-IA 16 T8027 ST.4a ~s ST-829 ST-I 128 135-A2° 19722
CD5 CD4 CD8 CD2 CD2
181
B5-522 B 18 151 S-MHCII TIITS 23 175 FI50 SB-I Namur 8CII 24 NAM 1 ( 17A3 ) NAM2 (11F5) NAM3 (1H8) NAM4 (2G 1)
CD59 CD45 (LCA) MHCII LFA-3, CD58 CDI la, LFA-I MHC II CD5
Kitasato BLMo-425 BLM0-1025 ~Baldwin et al., 1986. 2Ellis et al., 1988. 3Baldwin et al., 1988.4MacHugh et al., 1988.5Howard et al., 1988. 6Davis et al., 1988. 1Mackay et al., 1988b. aMaddox et al., 1985b. 9Mackay et al., 1985. '°Mackay et al., 1986. ~2Maddox et al., 1985a. 22Maddox et al., 1987. 23Mackay et al., 1987. 24Mackay et al., 1988c. ~SRabinovsky and Yang, 1986. 26Beya et al., 1986. mTMiyasaka et al., 1983. maMackay et al., 1988a. 29Ezaki et al., 1987.2°Giegerick et al., 1989. 2~McClure et al., 1989. 22Hein et al., 1988.23Hunig, 1985.24Depelchin et al., 1989. 25Kunita et al., 1988.
REFERENCES Baldwin, C.L., Teale, A.J., Naessens, J.G., Goddeeris, B.M., MacHugh, N.D. and Morrison, W.I., 1986. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and mufine L3T4. J. Immunol., 136: 4385-4391. Baldwin, C.L., MacHugh, N.D., Ellis, J.A., Naessens, J., Newson, J. and Morrison, W.I., 1988. Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens. Immunology, 63: 439-446.
10 Beya, M.F., Miyasaka, M., Dudler, L., Ezaki, T. and Trnka, Z., 1986. Studies on the differentiation of T lymphocytes in sheep. II. Two monoclonal antibodies that recognize all ovine T lymphocytes. Immunology, 57:115-121. Davis, W.C., Ellis, J.A., MacHugh, N.D. and Baldwin, C.L., 1988. Bovine pan T-cell monoclonal antibodies reactive with a molecule similar to CD2. Immunology, 63:165-167. Depelchin, A., Letesson, J.-J., Lostrie, N., Mammerikx, M., Portetelle, D. and Burny, A., 1989. Bovine leukaemia virus (BLV) infected B-cells express a marker similar to the CD5 T-cell marker. Immunol. Letters, 20: 69-76. Ellis, J.A., Baldwin, C.L., MacHugh, N.D., Bensaid, A., Teale, A.J., Goddeeris, B.M. and Morrison, W.I., 1986. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology, 58:351-358. Ellis, J.A., Davis, W.C., MacHugh, N.D., Emery, D.L., Kaushal, A. and Morrison, W.I., 1988. Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies. Vet. Immunol. lmmunopathol., 19: 325-340. Ezaki, T., Miyasaka, M., Beya, M.-F., Dudler, L. and Trnka, Z., 1987. A murine anti-sheep T8 monoclonal antibody, ST-~, ~hat defines the cytoto~,ic T 'yn:phocyte population, int. ~,rch. All. Appl. Immunol., 82:168-177. Giegerich, G.W., Hein, W.R., Miyasaka, M., Tiefenthaler, G. and Hunig, T., 1989. Restricted expression of CD2 among subsets of sheep thymocytes and T lymphocytes. Immunology, 66: 354-361. Hein, W.R., McClure, S., Beya, M.-F., Dudler, L. and Trnka, Z., 1988. A novel glycoprotein expressed on sheep T and B lymphocytes is involved in a T cell activation pathway. J. Immunol., 140: 2869-2875. Howard, C.J., Parsons, K.R., Jones, B.V., Sopp, P. and Pocock, D.H., 1988. Two monoclonal antibodies (CCI 7, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol., 19:127-139. Hunig, T., 1985. The cell surface molecule recognized by the erythrocyte receptor ofT lymphocytes: Identification and partial characterization using a monoclonal antibody. J. Exp. Med., 162: 890-901. Kunita, S., Koyama, H. and Saito, H., 1988. Preparation and characterization of monoc!ona! antibodies against bovine B lymphocyte surface antigens. Vet. Immunol. Immunopathol., 18: 201-212. MacHugh, N.D., Bensaid, A., Davis. W.C., Howard, C.J., Parsons, K.R., Jones, B. and Kaushal, A., 1988. Characterization of a bovine thymic differentiation antigen analogous to CDI in the human. Scand. J. Immunol., 27: 541-547. Mackay, C., 1988. Sheep leukocyte molecules: a review of their distribution, structure and possible function. Vet. Immunol. Immunopathol., 19: 1-20. Mackay, C.R., Maddox, J.F., Gogolin-Ewens, K.J. and Brandon, M.R., 1985. Characterization of two sheep lymphocyte differentiation antigens, SBU-TI and SBU-T6. Immunology, 55: 729-737. Mackay, C.R., Maddox, J.F., Brandon, M.R., 1986. Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol., 16:19-25. Mackay, C.R., Maddox, J.F., Brandon, M.R., 1987. A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol., 110: 46-55. Mackay, C.R., Hein, W.R., Brown, M.H. and Matzinger, P., 1988. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol., 18:1681-1688. MacKay, C.R., Maddox, J.F., Wijffels, G.L., Mackay, I.R. and Walker, I.D., 1988. Characterisation of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44. Immunology, 65: 93-99.
II McClure, S.J., Hein, W.R., Yamaguchi, K., Dudler, L., Beya, M.-F. and Miyasaka, M., 1989. Ontogeny, morphology and tissue distribution of a unique subset of CD4- CD8- sheep T lymphocytes. Immunol. Cell Biol., 67:215-221. Maddox, J.F., Mackay, C.R. and Brandon, M.R., 1985. The sheep analogue of leucocyte common antigen (LCA). Immunology, 55: 347-353. Maddox, J.F., Mackay, C.R. and Brandon, M.R., 1985b. Surface antigens, SBU-T4 and SBUTS, of sheep T lymphocyte subsets defined by monoclonal antibodies. Immunology, 55: 739748. Maddox, J.F., Mackay, C.R. and Brandon, M.R.. 1987. Ontogeny of ovine lymphocytes. I. An immunohistologic~l study on the development of T lymphocytes in the sheep embryo and fetal thymus. Immunology, 62: 97-105. Miyasaka, M., Heron, I., Dudler, L., Cahill, R.N.P., Forni, L., Knaak, T. and Trnka, Z., 1983. Studies on the differentiation of T lymphocytes in sheep. I. Recognition of a sheep T-lymphocyte differentiation antigen by a monoclonal antibody T-80. Immunology, 49: 545-553. Rabinovsky, E.D. and Yang, T.-J., 1986. Production and characterization of a bovine T cellspecific monoclonal antibody identifying a mature differentiation antigen. J. Immunol., 136: 609-615.
