RESEARCH ARTICLE
Leukocyte Mitochondrial DNA Copy Number Is Associated with Chronic Obstructive Pulmonary Disease Shih-Feng Liu1,2,3*, Ho-Chang Kuo2,3,4, Ching-Wan Tseng2, Hung-Tu Huang5, YungChe Chen1,3, Chia-Cheng Tseng1,3, Meng-Chih Lin1,2,3* 1 Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 2 Department of Respiratory Therapy, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 3 Chang Gung University College of Medicine, Kaohsiung, Taiwan, 4 Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 5 Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan * [email protected] (S-FL); [email protected] (M-CL)
a11111
Abstract Background
OPEN ACCESS Citation: Liu S-F, Kuo H-C, Tseng C-W, Huang H-T, Chen Y-C, Tseng C-C, et al. (2015) Leukocyte Mitochondrial DNA Copy Number Is Associated with Chronic Obstructive Pulmonary Disease. PLoS ONE 10(9): e0138716. doi:10.1371/journal.pone.0138716 Editor: Yunchao Su, Georgia Regents University, UNITED STATES Received: April 4, 2015 Accepted: September 2, 2015 Published: September 22, 2015 Copyright: © 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This study was supported by a grant from the Chang Gung Memorial Hospital (CMRPG891051). Competing Interests: The authors have declared that no competing interests exist.
Oxidative stress is known to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Evidence suggests that leukocytes mitochondria DNA (mtDNA) is susceptible to undergo mutations, insertions, or depletion in response to reactive oxidative stress (ROS). We hypothesize that mtDNA copy number is associated with the development of COPD.
Methodology/Principal Findings Relative mtDNA copy number was measured by a quantitative real-time PCR assay using DNA extracted from peripheral leukocytes. MtDNA copy number of peripheral leukocytes in the COPD group (n = 86) is significantly decreased compared with non-smoker group (n = 77) (250.3± 21.5 VS. 464.2± 49.9, P
Leukocyte Mitochondrial DNA Copy Number Is Associated with Chronic Obstructive Pulmonary Disease Shih-Feng Liu1,2,3*, Ho-Chang Kuo2,3,4, Ching-Wan Tseng2, Hung-Tu Huang5, YungChe Chen1,3, Chia-Cheng Tseng1,3, Meng-Chih Lin1,2,3* 1 Division of Pulmonary & Critical Care Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 2 Department of Respiratory Therapy, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 3 Chang Gung University College of Medicine, Kaohsiung, Taiwan, 4 Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, 5 Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan * [email protected] (S-FL); [email protected] (M-CL)
a11111
Abstract Background
OPEN ACCESS Citation: Liu S-F, Kuo H-C, Tseng C-W, Huang H-T, Chen Y-C, Tseng C-C, et al. (2015) Leukocyte Mitochondrial DNA Copy Number Is Associated with Chronic Obstructive Pulmonary Disease. PLoS ONE 10(9): e0138716. doi:10.1371/journal.pone.0138716 Editor: Yunchao Su, Georgia Regents University, UNITED STATES Received: April 4, 2015 Accepted: September 2, 2015 Published: September 22, 2015 Copyright: © 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This study was supported by a grant from the Chang Gung Memorial Hospital (CMRPG891051). Competing Interests: The authors have declared that no competing interests exist.
Oxidative stress is known to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Evidence suggests that leukocytes mitochondria DNA (mtDNA) is susceptible to undergo mutations, insertions, or depletion in response to reactive oxidative stress (ROS). We hypothesize that mtDNA copy number is associated with the development of COPD.
Methodology/Principal Findings Relative mtDNA copy number was measured by a quantitative real-time PCR assay using DNA extracted from peripheral leukocytes. MtDNA copy number of peripheral leukocytes in the COPD group (n = 86) is significantly decreased compared with non-smoker group (n = 77) (250.3± 21.5 VS. 464.2± 49.9, P
