Cerebellum (2015) 14:12–14 DOI 10.1007/s12311-014-0628-6
REVIEW
LGI1 is Involved in the Development of Mouse Brain Li-Da Su & Ya-Jun Xie & Liang Zhou & Ying Shen & Ying-Hong Hu
Published online: 5 December 2014 # Springer Science+Business Media New York 2014
Abstract Mutations in leucine-rich glioma inactivated 1 (LGI1) are linked to human autosomal dominant lateral temporal lobe epilepsy. It has been shown that LGI1 prevents the inactivation of voltage-gated potassium channels, mediates postnatal maturation of glutamatergic synapses, and regulates excitatory neurotransmission. However, other functions of LGI1 in the central nervous system have not been elucidated. We found that LGI1 is involved in the development of the cerebellum and cortex. The thickness of external granule layer was reduced, and foliation was affected in the cerebellum of LGI1 knockout mice. Double staining with Pax6 and BrdU showed a significant inhibition of proliferation of granule cell precursors of knockout embryos. The differentiation of radial glia cells was also suppressed in knockout mice, as shown by increased radial glial cells and decreased Bergmann glias in the areas of the cerebellum and cortex. Thus, our data demonstrate that LGI1 may be an essential player in the development of the brain.
Keywords LGI1 . Cerebellum . GFAP . Pax6 . BLBP
Li-Da Su and Ya-Jun Xie contributed equally to this work. L.
REVIEW
LGI1 is Involved in the Development of Mouse Brain Li-Da Su & Ya-Jun Xie & Liang Zhou & Ying Shen & Ying-Hong Hu
Published online: 5 December 2014 # Springer Science+Business Media New York 2014
Abstract Mutations in leucine-rich glioma inactivated 1 (LGI1) are linked to human autosomal dominant lateral temporal lobe epilepsy. It has been shown that LGI1 prevents the inactivation of voltage-gated potassium channels, mediates postnatal maturation of glutamatergic synapses, and regulates excitatory neurotransmission. However, other functions of LGI1 in the central nervous system have not been elucidated. We found that LGI1 is involved in the development of the cerebellum and cortex. The thickness of external granule layer was reduced, and foliation was affected in the cerebellum of LGI1 knockout mice. Double staining with Pax6 and BrdU showed a significant inhibition of proliferation of granule cell precursors of knockout embryos. The differentiation of radial glia cells was also suppressed in knockout mice, as shown by increased radial glial cells and decreased Bergmann glias in the areas of the cerebellum and cortex. Thus, our data demonstrate that LGI1 may be an essential player in the development of the brain.
Keywords LGI1 . Cerebellum . GFAP . Pax6 . BLBP
Li-Da Su and Ya-Jun Xie contributed equally to this work. L.
