Anal Bioanal Chem DOI 10.1007/s00216-015-8554-0
RESEARCH PAPER
Liquid chromatography tandem mass spectrometry quantitation of intracellular concentrations of ganciclovir and its phosphorylated forms Pierre-André Billat & François-Ludovic Sauvage & Nicolas Picard & Naїma Tafzi & Sophie Alain & Marie Essig & Pierre Marquet & Franck Saint-Marcoux
Received: 6 November 2014 / Revised: 6 January 2015 / Accepted: 10 February 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract Ganciclovir (GCV) is prescribed for cytomegalovirus infection which is a major issue in immunodepressed patients. It is however characterized by hematological toxicity. A better understanding of GCV concentration-effects relationships implies the measurement of intracellular forms. The objective of this study was to develop a method to measure GCV and its derivatives in cells. A four-stage procedure was developed with the following strategy: (1) to separate into different Parts of this work BDiscussed Poster Abstracts (2014). Fundamental & Clinical Pharmacology vol 28. doi: 10.1111/fcp.12065^ were presented at the joint 18th Annual Meeting of French Society of Pharmacology and Therapeutics and 81th Annual Meeting of Society of Physiology [1]. P.
RESEARCH PAPER
Liquid chromatography tandem mass spectrometry quantitation of intracellular concentrations of ganciclovir and its phosphorylated forms Pierre-André Billat & François-Ludovic Sauvage & Nicolas Picard & Naїma Tafzi & Sophie Alain & Marie Essig & Pierre Marquet & Franck Saint-Marcoux
Received: 6 November 2014 / Revised: 6 January 2015 / Accepted: 10 February 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract Ganciclovir (GCV) is prescribed for cytomegalovirus infection which is a major issue in immunodepressed patients. It is however characterized by hematological toxicity. A better understanding of GCV concentration-effects relationships implies the measurement of intracellular forms. The objective of this study was to develop a method to measure GCV and its derivatives in cells. A four-stage procedure was developed with the following strategy: (1) to separate into different Parts of this work BDiscussed Poster Abstracts (2014). Fundamental & Clinical Pharmacology vol 28. doi: 10.1111/fcp.12065^ were presented at the joint 18th Annual Meeting of French Society of Pharmacology and Therapeutics and 81th Annual Meeting of Society of Physiology [1]. P.
