Proc. Nati. Acad. Sci. USA Vol. 89, pp. 6452-6456, July 1992 Medical Sciences

Long-chain fatty acids activate calcium channels in ventricular myocytes (free fatty acids/arachidonic acid/olekic add)

JAMES MIN-CHE HUANG, Hu XIAN, AND MARVIN BACANER* Department of Physiology, University of Minnesota, Minneapolis, MN 55455

Communicated by James Serrin, March 30, 1992 (received for review September 15, 1991)

down (>70%o decrease in ICa) during the 10- to 15-min control period were discarded. Whole-cell voltage-clamp experiments with myocytes were done at 320C with 2- to 3-MU glass pipettes (Narishige PB 7 puller). Dagan 3900A patch-clamp circuit, axon DMA interface, IBM cloned AT 386 computer, and P-CLAMP software were used for command pulses, data acquisition, and analysis. All lipophilic agents were dissolved in 95% ethanol to make stock solutions and then diluted to

Long-chain fatty acids activate calcium channels in ventricular myocytes.

Nonesterified fatty acids accumulate at sites of tissue injury and necrosis. In cardiac tissue the concentrations of oleic acid, arachidonic acid, leu...
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