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CORRECTIONS Mechanism of action of purpuromycin P. LANDINI, E. CORTI, B. P. GOLDSTEIN and M. DENARO Volume 284 (1992), pages 47-52 The authors wish to make the following statement: Based on data from the literature [1], we used [3H]methionine to label yeast proteins. This amino acid is, indeed, incorporated to a greater extent than others we have tried. However, we recently observed that 20,cg of anisomycin/ml, a known inhibitor of eukaryotic protein synthesis [2], significantly inhibited leucine uptake, but not methionine uptake, in Candida albicans. This led us to re-evaluate purpuromycin. We found that 10 jtg of purpuromycin/ml inhibited leucine incorporation by almost 90 % in a 15 min exposure. The lack of inhibition of methionine uptake is probably due to incorporation of the labelled methyl
group into other macromolecules. We are currently attempting to determine where the methyl group of methionine is incorporated in the presence and the absence of protein inhibitors. We are at present unable to explain the lack of activity of purpuromycin in the C. albicans cell-free protein synthesis system. 1. Cooper, T. G. (1982) in The Molecular Biology of the Yeast
Saccharomyces: Metabolism and Gene Expression (Strathern, J. N., Jones, F. W. & Broach, J. R., eds.), pp. 399-461, Cold Spring Harbor Laboratory Press, Cold Spring Harbor 2. Grollman, A. P. (1967) J. Biol. Chem. 242, 3226-3233
The C-terminal domain of 72 kDa gelatinase A is not required for catalysis, but is essential for membrane activation and modulates interactions with tissue inhibitors of metalloproteinases G. MURPHY, F. WILLENBROCK, R. V. WARD, M. I. COCKETT, D. EATON and A. J. P. DOCHERTY Volume 283 (1992) page 637, second column, lines 3-4: for the enzyme coded for by gelatinase A cDNA lacking nucleotides 418-631 read the enzyme coded for by gelatinase A cDNA lacking amino acids 418-631 page 637, second column, line 8 from bottom: for C. Gafton read C. Gofton page 637, second column, line 3 from bottom: for The conversion of adenosine-418 into a stop codon read The conversion of alanine418 into a stop codon
CORRECTIONS Mechanism of action of purpuromycin P. LANDINI, E. CORTI, B. P. GOLDSTEIN and M. DENARO Volume 284 (1992), pages 47-52 The authors wish to make the following statement: Based on data from the literature [1], we used [3H]methionine to label yeast proteins. This amino acid is, indeed, incorporated to a greater extent than others we have tried. However, we recently observed that 20,cg of anisomycin/ml, a known inhibitor of eukaryotic protein synthesis [2], significantly inhibited leucine uptake, but not methionine uptake, in Candida albicans. This led us to re-evaluate purpuromycin. We found that 10 jtg of purpuromycin/ml inhibited leucine incorporation by almost 90 % in a 15 min exposure. The lack of inhibition of methionine uptake is probably due to incorporation of the labelled methyl
group into other macromolecules. We are currently attempting to determine where the methyl group of methionine is incorporated in the presence and the absence of protein inhibitors. We are at present unable to explain the lack of activity of purpuromycin in the C. albicans cell-free protein synthesis system. 1. Cooper, T. G. (1982) in The Molecular Biology of the Yeast
Saccharomyces: Metabolism and Gene Expression (Strathern, J. N., Jones, F. W. & Broach, J. R., eds.), pp. 399-461, Cold Spring Harbor Laboratory Press, Cold Spring Harbor 2. Grollman, A. P. (1967) J. Biol. Chem. 242, 3226-3233
The C-terminal domain of 72 kDa gelatinase A is not required for catalysis, but is essential for membrane activation and modulates interactions with tissue inhibitors of metalloproteinases G. MURPHY, F. WILLENBROCK, R. V. WARD, M. I. COCKETT, D. EATON and A. J. P. DOCHERTY Volume 283 (1992) page 637, second column, lines 3-4: for the enzyme coded for by gelatinase A cDNA lacking nucleotides 418-631 read the enzyme coded for by gelatinase A cDNA lacking amino acids 418-631 page 637, second column, line 8 from bottom: for C. Gafton read C. Gofton page 637, second column, line 3 from bottom: for The conversion of adenosine-418 into a stop codon read The conversion of alanine418 into a stop codon
