Neuroscience Letters. 141 (1992) 243 246 c~ 1992 Elsevier Scientific Publishers Ireland Ltd. All rights reserved 0304-3940/92/$ 05.00
243
NSL 08771
Methionine enkephalin accumulates in plasma but not in brain or cerebrospinal fluid of rats with acute toxic hepatitis M a r k G. Swain", Melvyn R Heyes b, John Vergalla ~ and E. A n t h o n y Jones" "Liver Diseases Section, N1DDK and hSeetion on Anah'tieal Biochemistry, Laborato O, o[' Clh~ieal Seience, NIMH, National ln.vtitules o/Health, Bethesda, MD 20892 (USA) (Received 26 February 1992; Revised version received 21 April 1992: Accepted 22 April 1992) Key wor&v
Methionine enkephalin: Toxic hepatitis: Blood-to-brain transfer
In order to determine whether acute toxic hepatitis in the rat is associated with an accumulation of methionine enkephalin in plasma and increased blood-to-brain transfer of methionine enkephalin, immunoreactive methionine enkephalin levels were determined by radioimmunoassay in plasma, cerebrospinal fluid and whole brain samples from rats with thioacetamide induced acute toxic hepatitis. Thioacetamide treatment was associated with an 8.7-fold increase in plasma immunoreactive methionine enkephalin levels (P
243
NSL 08771
Methionine enkephalin accumulates in plasma but not in brain or cerebrospinal fluid of rats with acute toxic hepatitis M a r k G. Swain", Melvyn R Heyes b, John Vergalla ~ and E. A n t h o n y Jones" "Liver Diseases Section, N1DDK and hSeetion on Anah'tieal Biochemistry, Laborato O, o[' Clh~ieal Seience, NIMH, National ln.vtitules o/Health, Bethesda, MD 20892 (USA) (Received 26 February 1992; Revised version received 21 April 1992: Accepted 22 April 1992) Key wor&v
Methionine enkephalin: Toxic hepatitis: Blood-to-brain transfer
In order to determine whether acute toxic hepatitis in the rat is associated with an accumulation of methionine enkephalin in plasma and increased blood-to-brain transfer of methionine enkephalin, immunoreactive methionine enkephalin levels were determined by radioimmunoassay in plasma, cerebrospinal fluid and whole brain samples from rats with thioacetamide induced acute toxic hepatitis. Thioacetamide treatment was associated with an 8.7-fold increase in plasma immunoreactive methionine enkephalin levels (P
