S220
EPIDEMIOLOGY AND SOCIAL SCIENCE PERSPECTIVES
Microbiology and Diagnosis of Infections with Shigella and Enteroinvasive Escherichia coli Peter Echeverria, Omtipa Sethabutr, and Chittima Pitarangsi
From the Department of Bacteriology, Armed Forces Research Institute of Medical Sciences, Bangkok. Thailand
The etiology of dysentery in Thailand and the existing methods of diagnosing infections with
Shigella and enteroinvasive Escherichia coli (EIEC) are reviewed. The four Shigella species (S. dysenteriae, S. ftexneri, S. boydU, and S. sonner) are classically identified by culture of fecal speci-
Bacillary dysentery is a localized, ulcerative infection of the colon characterized by abdominal pain and the frequent passage of stools containing blood and mucus [1, 2]. A number of bacterial species, including Shigella [3], Escherichia coli [4], Campylobacter [5], Salmonella [6], Vibrio [7], Aeromonas [8, 9], and Plesiomonas [9, 10], have been isolated from patients with acute colitis. In studies of diarrheal disease in children in Thailand, Shigella, Salmonella, and enteroinvasive E. coli (EIEC) were isolated more often from patients with mucoid and bloody diarrhea than from those with watery diarrhea [11]. Salmonella, which is firmly established in the food chain in Thailand [12], was associated with diarrhea only in children 3% dehydration ~ 10 WBCs/hpf Occult blood ORS treatment Antibiotic treatment Intravenous fluid treatment Hospitalization
Rotavirus (n = 141)
Echeverria, Sethabutr, and Pitarangsi
S224
Table S. Detection of Shigella and EIEC by examination of stool blots with radiolabeled and biotinylated probes. No. positive in indicated assay* Group, type of infection Children with diarrhea Shigella EIEC Nonef Children without diarrhea Shigella EIEC Nonef
Standard
32p
BiotinII-dUTP
Pvalue t
155 19 1,056
94 13 14
59 10 71
EPIDEMIOLOGY AND SOCIAL SCIENCE PERSPECTIVES
Microbiology and Diagnosis of Infections with Shigella and Enteroinvasive Escherichia coli Peter Echeverria, Omtipa Sethabutr, and Chittima Pitarangsi
From the Department of Bacteriology, Armed Forces Research Institute of Medical Sciences, Bangkok. Thailand
The etiology of dysentery in Thailand and the existing methods of diagnosing infections with
Shigella and enteroinvasive Escherichia coli (EIEC) are reviewed. The four Shigella species (S. dysenteriae, S. ftexneri, S. boydU, and S. sonner) are classically identified by culture of fecal speci-
Bacillary dysentery is a localized, ulcerative infection of the colon characterized by abdominal pain and the frequent passage of stools containing blood and mucus [1, 2]. A number of bacterial species, including Shigella [3], Escherichia coli [4], Campylobacter [5], Salmonella [6], Vibrio [7], Aeromonas [8, 9], and Plesiomonas [9, 10], have been isolated from patients with acute colitis. In studies of diarrheal disease in children in Thailand, Shigella, Salmonella, and enteroinvasive E. coli (EIEC) were isolated more often from patients with mucoid and bloody diarrhea than from those with watery diarrhea [11]. Salmonella, which is firmly established in the food chain in Thailand [12], was associated with diarrhea only in children 3% dehydration ~ 10 WBCs/hpf Occult blood ORS treatment Antibiotic treatment Intravenous fluid treatment Hospitalization
Rotavirus (n = 141)
Echeverria, Sethabutr, and Pitarangsi
S224
Table S. Detection of Shigella and EIEC by examination of stool blots with radiolabeled and biotinylated probes. No. positive in indicated assay* Group, type of infection Children with diarrhea Shigella EIEC Nonef Children without diarrhea Shigella EIEC Nonef
Standard
32p
BiotinII-dUTP
Pvalue t
155 19 1,056
94 13 14
59 10 71
